##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AclI AflIII AscI AseI AsnI
BsaBI BsiWI BspDI BspLU11I BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoO65I EcoRV EcoT22I HpaI MamI
MfeI MluI MunI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshBI Psp1406I RsrII SfiI
SfuI SnaBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 2920 GACGTC GACGT^C
Acc65I 2856 GGTACC G^GTACC
AccIII 7641 TCCGGA T^CCGGA
Aor51HI 7923 AGCGCT AGC^GCT
Asp718I 2856 GGTACC G^GTACC
AviII 8522 TGCGCA TGC^GCA
BamHI 3035 GGATCC G^GATCC
BglII 1755 AGATCT A^GATCT
BseAI 7641 TCCGGA T^CCGGA
BsiEI 6357 CGATCG CG[GA][CT]^CG
Bsp1407I 7557 TGTACA T^GTACA
BspEI 7641 TCCGGA T^CCGGA
BspHI 1916 TCATGA T^CATGA
BsrGI 7557 TGTACA T^GTACA
BssHII 6169 GCGCGC G^CGCGC
DrdI 181 GACCGGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 7923 AGCGCT AGC^GCT
FspI 8522 TGCGCA TGC^GCA
HindIII 298 AAGCTT A^AGCTT
KpnI 2856 GGTACC GGTAC^C
KspI 4637 CCGCGG CCGC^GG
MroI 7641 TCCGGA T^CCGGA
NdeI 7236 CATATG CA^TATG
PshAI 3582 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 6357 CGATCG CGAT^CG
RcaI 1916 TCATGA T^CATGA
SacII 4637 CCGCGG CCGC^GG
SalI 5663 GTCGAC G^TCGAC
SgrAI 23 CGCCGGCG C[GA]^CCGG[CT]G
SphI 4667 GCATGC GCATG^C
SspBI 7557 TGTACA T^GTACA
XbaI 8006 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 2443 GTCTAC GT^[AC][GT]AC
5663 GTCGAC
AflII 5413 CTTAAG C^TTAAG
5710 CTTAAG
AgeI 182 ACCGGT A^CCGGT
8111 ACCGGT
AhdI 3202 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5146 GACGACAAGTC
AspEI 3202 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5146 GACGACAAGTC
AvrII 648 CCTAGG C^CTAGG
8208 CCTAGG
BbrPI 6938 CACGTG CAC^GTG
7946 CACGTG
BcgI 2309 CGACCCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4317 CGAGCCCAATGC
BclI 785 TGATCA T^GATCA
6855 TGATCA
BfrI 5413 CTTAAG C^TTAAG
5710 CTTAAG
BlnI 648 CCTAGG C^CTAGG
8208 CCTAGG
BsmBI 1187 CGTCTC CGTCTC(1/5)
6077 GAGACG
BsrBI 270 CCGCTC CCGCTC(-3/-3)
2797 GAGCGG
DraI 8431 TTTAAA TTT^AAA
8899 TTTAAA
Eam1105I 3202 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5146 GACGACAAGTC
FbaI 785 TGATCA T^GATCA
6855 TGATCA
FseI 8 GGCCGGCC GGCCGG^CC
5032 GGCCGGCC
PinAI 182 ACCGGT A^CCGGT
8111 ACCGGT
PmaCI 6938 CACGTG CAC^GTG
7946 CACGTG
PmlI 6938 CACGTG CAC^GTG
7946 CACGTG
SbfI 2664 CCTGCAGG CCTGCA^GG
4494 CCTGCAGG
ScaI 2507 AGTACT AGT^ACT
8474 AGTACT
SpeI 1305 ACTAGT A^CTAGT
8454 ACTAGT
Sse8387I 2664 CCTGCAGG CCTGCA^GG
4494 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BciVI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI