##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI Asp718I AviII BbeI
BcgI BseAI BsiWI BspDI BspEI
BspHI BsrBI BssHII BssSI BstBI
BstEII BstPI ClaI CpoI Eco47III
EcoO65I FseI FspI HpaI KasI
KpnI MluI MroI NarI NspV
PacI PaeR7I PinAI PmeI Psp1406I
PvuI RcaI RsrII SbfI SexAI
SfiI SfoI SfuI SnaBI SpeI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 7459 GTGCAC G^TGCAC
ApaLI 7459 GTGCAC G^TGCAC
BalI 6033 TGGCCA TGG^CCA
BamHI 6723 GGATCC G^GATCC
BbrPI 208 CACGTG CAC^GTG
BglI 3052 GCCATTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 6449 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 5386 GGTCTC GGTCTC(1/5)
BsaWI 4939 ACCGGA [AT]^CCGG[AT]
BstXI 7143 CCAGCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 912 GACAGATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 7813 GATATC GAT^ATC
HaeII 116 AGCGCC [GA]GCGC^[CT]
MamI 6449 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1310 CAATTG C^AATTG
MluNI 6033 TGGCCA TGG^CCA
MscI 6033 TGGCCA TGG^CCA
MunI 1310 CAATTG C^AATTG
NaeI 189 GCCGGC GCC^GGC
NgoMIV 189 GCCGGC G^CCGGC
NheI 1804 GCTAGC G^CTAGC
NotI 96 GCGGCCGC GC^GGCCGC
NruI 222 TCGCGA TCG^CGA
PmaCI 208 CACGTG CAC^GTG
PmlI 208 CACGTG CAC^GTG
SalI 19 GTCGAC G^TCGAC
ScaI 7259 AGTACT AGT^ACT
SgrAI 214 CGCCGGTG C[GA]^CCGG[CT]G
SspI 5407 AATATT AAT^ATT
StuI 4279 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflII 2126 CTTAAG C^TTAAG
6044 CTTAAG
AhdI 4522 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4985 GACAATGTGTC
ApaI 228 GGGCCC GGGCC^C
3290 GGGCCC
AseI 1036 ATTAAT AT^TAAT
5114 ATTAAT
AsnI 1036 ATTAAT AT^TAAT
5114 ATTAAT
AspEI 4522 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4985 GACAATGTGTC
AvrII 6391 CCTAGG C^CTAGG
7061 CCTAGG
BciVI 3560 GTATCC GTATCC(6/5)
4250 GTATCC
BclI 5416 TGATCA T^GATCA
7771 TGATCA
BfrI 2126 CTTAAG C^TTAAG
6044 CTTAAG
BlnI 6391 CCTAGG C^CTAGG
7061 CCTAGG
BlpI 2318 GCTAAGC GC^T[ACGT]AGC
3172 GCTTAGC
Bpu1102I 2318 GCTAAGC GC^T[ACGT]AGC
3172 GCTTAGC
BsaAI 208 CACGTG [CT]AC^GT[GA]
6335 CACGTA
BsiEI 97 CGGCCG CG[GA][CT]^CG
250 CGGCCG
BsmBI 4394 GAGACG CGTCTC(1/5)
6632 GAGACG
BsmI 3224 GCATTC GAATGC(1/-1)
6952 GCATTC
Bst1107I 372 GTATAC GTA^TAC
776 GTATAC
BstAPI 8615 GCATACGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
8624 GCACATTATGC
BstZ17I 372 GTATAC GTA^TAC
776 GTATAC
CelII 2318 GCTAAGC GC^T[ACGT]AGC
3172 GCTTAGC
EagI 97 CGGCCG C^GGCCG
250 CGGCCG
Eam1105I 4522 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4985 GACAATGTGTC
EclXI 97 CGGCCG C^GGCCG
250 CGGCCG
Eco52I 97 CGGCCG C^GGCCG
250 CGGCCG
FbaI 5416 TGATCA T^GATCA
7771 TGATCA
NcoI 815 CCATGG C^CATGG
1022 CCATGG
NdeI 2928 CATATG CA^TATG
3344 CATATG
PshAI 3126 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5573 GACTTCAGTC
PshBI 1036 ATTAAT AT^TAAT
5114 ATTAAT
PstI 3507 CTGCAG CTGCA^G
5855 CTGCAG
SacI 3464 GAGCTC GAGCT^C
3940 GAGCTC
SphI 2025 GCATGC GCATG^C
8596 GCATGC
XbaI 4558 TCTAGA T^CTAGA
6457 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI Asp700I AspHI AspI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglII
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflFI
PflMI PleI PpuMI PvuII RsaI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI