##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AccI AccIII AgeI AscI AviII
BbeI BseAI BsiEI BsiWI BspDI
BspEI BspHI BssHII BssSI Bst1107I
BstZ17I ClaI CpoI DrdI EagI
EclXI Eco52I FseI FspI KasI
KspI MfeI MluI MroI MunI
NarI NotI NruI PacI PinAI
PmeI PvuI RcaI RsrII SacII
SalI SbfI SfoI SgrAI SnaBI
SpeI Sse8387I
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 6510 GACGTC GACGT^C
Acc65I 7892 GGTACC G^GTACC
AclI 2858 AACGTT AA^CGTT
AflII 8368 CTTAAG C^TTAAG
Alw44I 7641 GTGCAC G^TGCAC
ApaLI 7641 GTGCAC G^TGCAC
AseI 6705 ATTAAT AT^TAAT
AsnI 6705 ATTAAT AT^TAAT
Asp718I 7892 GGTACC G^GTACC
AvrII 3675 CCTAGG C^CTAGG
BcgI 942 CGATACTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2097 TGATCA T^GATCA
BfrI 8368 CTTAAG C^TTAAG
BlnI 3675 CCTAGG C^CTAGG
BsaWI 1910 TCCGGT [AT]^CCGG[AT]
Bsp1407I 1118 TGTACA T^GTACA
BspLU11I 1542 ACATGT A^CATGT
BsrBI 6313 CCGCTC CCGCTC(-3/-3)
BsrGI 1118 TGTACA T^GTACA
BstBI 3399 TTCGAA TT^CGAA
BstEII 1065 GGTGACC G^GT[ACGT]ACC
BstPI 1065 GGTGACC G^GT[ACGT]ACC
EcoO65I 1065 GGTGACC G^GT[ACGT]ACC
EcoRV 3963 GATATC GAT^ATC
FbaI 2097 TGATCA T^GATCA
KpnI 7892 GGTACC GGTAC^C
NaeI 566 GCCGGC GCC^GGC
NgoMIV 566 GCCGGC G^CCGGC
NheI 4893 GCTAGC G^CTAGC
NspV 3399 TTCGAA TT^CGAA
PaeR7I 3347 CTCGAG C^TCGAG
PshBI 6705 ATTAAT AT^TAAT
Psp1406I 2858 AACGTT AA^CGTT
ScaI 6685 AGTACT AGT^ACT
SfiI 4518 GGCCAGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3399 TTCGAA TT^CGAA
SspBI 1118 TGTACA T^GTACA
SwaI 1881 ATTTAAAT ATTT^AAAT
XbaI 9002 TCTAGA T^CTAGA
XhoI 3347 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 1997 GACGCC G[GA]^CG[CT]C
6510 GACGTC
AhdI 49 GACCGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1821 GACTTTCAGTC
Aor51HI 2942 AGCGCT AGC^GCT
4027 AGCGCT
AspEI 49 GACCGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1821 GACTTTCAGTC
AspI 6919 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
9076 GACTCTGTC
BbrPI 917 CACGTG CAC^GTG
8955 CACGTG
BciVI 4232 GGATAC GTATCC(6/5)
5821 GGATAC
BglII 4884 AGATCT A^GATCT
5519 AGATCT
BlpI 1240 GCTCAGC GC^T[ACGT]AGC
4123 GCTGAGC
Bpu1102I 1240 GCTCAGC GC^T[ACGT]AGC
4123 GCTGAGC
BsaBI 2249 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4186 GATGAAAATC
BsaHI 1997 GACGCC G[GA]^CG[CT]C
6510 GACGTC
BsmBI 7834 CGTCTC CGTCTC(1/5)
6430 GAGACG
BsmI 8567 GCATTC GAATGC(1/-1)
8585 GCATTC
BsrFI 566 GCCGGC [GA]^CCGG[CT]
8671 GCCGGT
CelII 1240 GCTCAGC GC^T[ACGT]AGC
4123 GCTGAGC
Cfr10I 566 GCCGGC [GA]^CCGG[CT]
8671 GCCGGT
DraIII 1780 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
7992 CACAGGGTG
Eam1105I 49 GACCGATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1821 GACTTTCAGTC
Eco47III 2942 AGCGCT AGC^GCT
4027 AGCGCT
HgaI 1997 GACGC GACGC(5/10)
3620 GACGC
Hin1I 1997 GACGCC G[GA]^CG[CT]C
6510 GACGTC
HindIII 1988 AAGCTT A^AGCTT
3215 AAGCTT
HpaI 2995 GTTAAC GTT^AAC
4661 GTTAAC
MamI 2249 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4186 GATGAAAATC
PflFI 6919 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
9076 GACTCTGTC
PmaCI 917 CACGTG CAC^GTG
8955 CACGTG
PmlI 917 CACGTG CAC^GTG
8955 CACGTG
PvuII 4497 CAGCTG CAG^CTG
5416 CAGCTG
Tth111I 6919 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
9076 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PshAI PstI RsaI SacI SapI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmaI SmlI SphI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI