##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp718I AspEI AspI AvrII
BbeI BcgI BfrI BlnI BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV FseI
HincII HindII HpaI KasI KpnI
MfeI MluI MunI NarI NdeI
NheI NotI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI SexAI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 2062 GTCTAC GT^[AC][GT]AC
AccIII 2633 TCCGGA T^CCGGA
AcsI 1069 AAATTC [GA]^AATT[CT]
AcyI 1521 GACGCC G[GA]^CG[CT]C
ApoI 1069 AAATTC [GA]^AATT[CT]
AseI 3006 ATTAAT AT^TAAT
AsnI 3006 ATTAAT AT^TAAT
AviII 36 TGCGCA TGC^GCA
BalI 2757 TGGCCA TGG^CCA
BamHI 219 GGATCC G^GATCC
BbrPI 895 CACGTG CAC^GTG
BbvCI 841 GCTGAGG CCTCAGC(-5/-2)
BciVI 469 GTATCC GTATCC(6/5)
BclI 1655 TGATCA T^GATCA
BglII 1094 AGATCT A^GATCT
BlpI 2073 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2073 GCTCAGC GC^T[ACGT]AGC
BsaBI 2557 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1521 GACGCC G[GA]^CG[CT]C
BseAI 2633 TCCGGA T^CCGGA
BsmBI 1423 GAGACG CGTCTC(1/5)
BsmI 2126 GCATTC GAATGC(1/-1)
BspEI 2633 TCCGGA T^CCGGA
BssSI 1555 CTCGTG CACGAG(-5/-1)
BstAPI 693 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2073 GCTCAGC GC^T[ACGT]AGC
DraIII 2703 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 618 ATGCAT ATGCA^T
FbaI 1655 TGATCA T^GATCA
FspI 36 TGCGCA TGC^GCA
Hin1I 1521 GACGCC G[GA]^CG[CT]C
KspI 1482 CCGCGG CCGC^GG
MamI 2557 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2757 TGGCCA TGG^CCA
MroI 2633 TCCGGA T^CCGGA
MscI 2757 TGGCCA TGG^CCA
NcoI 1361 CCATGG C^CATGG
NruI 2409 TCGCGA TCG^CGA
NsiI 618 ATGCAT ATGCA^T
PflMI 2240 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 895 CACGTG CAC^GTG
PmlI 895 CACGTG CAC^GTG
PshBI 3006 ATTAAT AT^TAAT
SacI 763 GAGCTC GAGCT^C
SacII 1482 CCGCGG CCGC^GG
ScaI 2435 AGTACT AGT^ACT
SfiI 1440 GGCCAATGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1595 GCATGC GCATG^C
SspI 685 AATATT AAT^ATT
Van91I 2240 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2743 TCTAGA T^CTAGA
XcmI 2990 CCATTCATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 181 GGGCCC GGGCC^C
2731 GGGCCC
Asp700I 537 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2463 GAAGTTCTTC
AspHI 448 GAGCAC G[AT]GC[AT]^C
763 GAGCTC
BfaI 313 CTAG C^TAG
2744 CTAG
BsaI 833 GGTCTC GGTCTC(1/5)
2717 GGTCTC
BsaWI 962 TCCGGT [AT]^CCGG[AT]
2633 TCCGGA
BsiHKAI 448 GAGCAC G[AT]GC[AT]^C
763 GAGCTC
BssHII 252 GCGCGC G^CGCGC
291 GCGCGC
BstXI 1361 CCATGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2450 CCAGCGCCCTGG
EcoNI 1279 CCTATGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2836 CCTGGCCCAGG
HaeII 1478 AGCGCC [GA]GCGC^[CT]
2452 AGCGCC
MaeI 313 CTAG C^TAG
2744 CTAG
MseI 791 TTAA T^TAA
3007 TTAA
NaeI 160 GCCGGC GCC^GGC
986 GCCGGC
NgoMIV 160 GCCGGC G^CCGGC
986 GCCGGC
NspI 1595 GCATGC [GA]CATG^[CT]
1694 GCATGT
StuI 2493 AGGCCT AGG^CCT
2795 AGGCCT
Tru9I 791 TTAA T^TAA
3007 TTAA
XmnI 537 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2463 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AvaI AvaII BanI
BanII BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI