##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AclI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI Asp700I AspEI
AspI AviII AvrII BbvCI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaI BsiWI BspDI BspHI
BspLU11I BspMI BssHII Bst1107I BstZ17I
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoT22I FbaI FseI FspI HpaI
KspI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
PacI PflFI PinAI PmeI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacII SapI SbfI ScaI SexAI
SfiI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 723 GACGTC GACGT^C
Acc65I 837 GGTACC G^GTACC
AccI 1239 GTCGAC GT^[AC][GT]AC
AccII 2031 CGCG CG^CG
AflIII 1675 ACGTGT A^C[GA][CT]GT
AseI 2881 ATTAAT AT^TAAT
AsnI 2881 ATTAAT AT^TAAT
Asp718I 837 GGTACC G^GTACC
BalI 1931 TGGCCA TGG^CCA
BbeI 74 GGCGCC GGCGC^C
BbrPI 1674 CACGTG CAC^GTG
BciVI 698 GGATAC GTATCC(6/5)
BglII 369 AGATCT A^GATCT
BsaAI 1674 CACGTG [CT]AC^GT[GA]
BsiEI 1237 CGGTCG CG[GA][CT]^CG
Bsp1407I 2781 TGTACA T^GTACA
BsrBI 1814 CCGCTC CCGCTC(-3/-3)
BsrGI 2781 TGTACA T^GTACA
BssSI 398 CTCGTG CACGAG(-5/-1)
BstAPI 1145 GCAGAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 956 TTCGAA TT^CGAA
BstEII 1371 GGTCACC G^GT[ACGT]ACC
BstPI 1371 GGTCACC G^GT[ACGT]ACC
BstUI 2031 CGCG CG^CG
BstXI 684 CCATCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 2823 CCATGG C^C[GA][CT]GG
EcoO65I 1371 GGTCACC G^GT[ACGT]ACC
EcoRI 2295 GAATTC G^AATTC
EcoRV 2828 GATATC GAT^ATC
HindIII 1923 AAGCTT A^AGCTT
KasI 74 GGCGCC G^GCGCC
KpnI 837 GGTACC GGTAC^C
MluNI 1931 TGGCCA TGG^CCA
MscI 1931 TGGCCA TGG^CCA
MvnI 2031 CGCG CG^CG
NarI 74 GGCGCC GG^CGCC
NcoI 2823 CCATGG C^CATGG
NspV 956 TTCGAA TT^CGAA
PflMI 1338 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1674 CACGTG CAC^GTG
PmlI 1674 CACGTG CAC^GTG
PshBI 2881 ATTAAT AT^TAAT
PvuII 553 CAGCTG CAG^CTG
SacI 20 GAGCTC GAGCT^C
SalI 1239 GTCGAC G^TCGAC
SfoI 74 GGCGCC GGC^GCC
SfuI 956 TTCGAA TT^CGAA
SgrAI 64 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1203 CCCGGG CCC^GGG
SphI 2132 GCATGC GCATG^C
SspBI 2781 TGTACA T^GTACA
Van91I 1338 CCAATCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1203 CCCGGG C^CCGGG
XmaI 1203 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 55 TCCGGA T^CCGGA
153 TCCGGA
AcyI 74 GGCGCC G[GA]^CG[CT]C
723 GACGTC
ApaI 1047 GGGCCC GGGCC^C
1994 GGGCCC
AspHI 20 GAGCTC G[AT]GC[AT]^C
280 GAGCAC
BamHI 156 GGATCC G^GATCC
1296 GGATCC
BbsI 507 GAAGAC GAAGAC(2/6)
755 GTCTTC
BcgI 1241 CGACCAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
6 GCATCTTTCTCG
BpuAI 507 GAAGAC GAAGAC(2/6)
755 GTCTTC
BsaHI 74 GGCGCC G[GA]^CG[CT]C
723 GACGTC
BsaWI 55 TCCGGA [AT]^CCGG[AT]
153 TCCGGA
BseAI 55 TCCGGA T^CCGGA
153 TCCGGA
BsiHKAI 20 GAGCTC G[AT]GC[AT]^C
280 GAGCAC
BspEI 55 TCCGGA T^CCGGA
153 TCCGGA
BsrDI 2501 CATTGC GCAATG(2/0)
2532 GCAATG
EaeI 748 CGGCCA [CT]^GGCC[GA]
1931 TGGCCA
EcoNI 1640 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2267 CCTTCTGAAGG
HgaI 301 GCGTC GACGC(5/10)
1630 GCGTC
Hin1I 74 GGCGCC G[GA]^CG[CT]C
723 GACGTC
HincII 494 GTTGAC GT[CT]^[GA]AC
1239 GTCGAC
HindII 494 GTTGAC GT[CT]^[GA]AC
1239 GTCGAC
MroI 55 TCCGGA T^CCGGA
153 TCCGGA
MspA1I 125 CAGCGG C[AC]G^C[GT]G
553 CAGCTG
NaeI 65 GCCGGC GCC^GGC
2128 GCCGGC
NgoMIV 65 GCCGGC G^CCGGC
2128 GCCGGC
PaeR7I 14 CTCGAG C^TCGAG
1291 CTCGAG
PstI 1407 CTGCAG CTGCA^G
1438 CTGCAG
StuI 652 AGGCCT AGG^CCT
2116 AGGCCT
XbaI 323 TCTAGA T^CTAGA
1089 TCTAGA
XcmI 390 CCATAGAACTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1084 CCAGTTCTAGAGTGG
XhoI 14 CTCGAG C^TCGAG
1291 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII