##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AviII BamHI
BbeI BcgI BclI BglI BsaBI
BseAI Bsp1407I BspEI BsrGI BssHII
Bst1107I BstBI BstZ17I CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SwaI Van91I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 513 CGCG CG^CG
AcyI 575 GACGCC G[GA]^CG[CT]C
AgeI 379 ACCGGT A^CCGGT
AocI 2279 CCTGAGG CC^T[ACGT]AGG
ApaI 1062 GGGCCC GGGCC^C
AspI 1719 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 912 CCTAGG C^CTAGG
BbrPI 1947 CACGTG CAC^GTG
BciVI 431 GTATCC GTATCC(6/5)
BlnI 912 CCTAGG C^CTAGG
BsaHI 575 GACGCC G[GA]^CG[CT]C
BsiWI 796 CGTACG C^GTACG
BsmBI 2784 GAGACG CGTCTC(1/5)
BspDI 1502 ATCGAT AT^CGAT
BspHI 397 TCATGA T^CATGA
BspLU11I 930 ACATGT A^CATGT
BsrBI 44 GAGCGG CCGCTC(-3/-3)
BsrDI 778 GCAATG GCAATG(2/0)
BsrFI 379 ACCGGT [GA]^CCGG[CT]
BstAPI 1985 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 705 GGTGACC G^GT[ACGT]ACC
BstPI 705 GGTGACC G^GT[ACGT]ACC
BstUI 513 CGCG CG^CG
Bsu36I 2279 CCTGAGG CC^T[ACGT]AGG
Cfr10I 379 ACCGGT [GA]^CCGG[CT]
ClaI 1502 ATCGAT AT^CGAT
DraI 588 TTTAAA TTT^AAA
Eco81I 2279 CCTGAGG CC^T[ACGT]AGG
EcoO65I 705 GGTGACC G^GT[ACGT]ACC
Hin1I 575 GACGCC G[GA]^CG[CT]C
MvnI 513 CGCG CG^CG
PflFI 1719 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 379 ACCGGT A^CCGGT
PmaCI 1947 CACGTG CAC^GTG
PmlI 1947 CACGTG CAC^GTG
PvuII 1732 CAGCTG CAG^CTG
RcaI 397 TCATGA T^CATGA
SalI 2811 GTCGAC G^TCGAC
SapI 3032 GAAGAGC GCTCTTC(1/4)
SexAI 151 ACCAGGT A^CC[AT]GGT
Tth111I 1719 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 1313 GTCTAC GT^[AC][GT]AC
2811 GTCGAC
AflII 744 CTTAAG C^TTAAG
3262 CTTAAG
AflIII 682 ACACGT A^C[GA][CT]GT
930 ACATGT
Asp700I 401 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3277 GAAGAGGTTC
BalI 232 TGGCCA TGG^CCA
3049 TGGCCA
BanI 884 GGTGCC G^G[CT][GA]CC
2149 GGCACC
BfrI 744 CTTAAG C^TTAAG
3262 CTTAAG
BglII 1603 AGATCT A^GATCT
3254 AGATCT
BlpI 41 GCTGAGC GC^T[ACGT]AGC
1902 GCTCAGC
Bpu1102I 41 GCTGAGC GC^T[ACGT]AGC
1902 GCTCAGC
BsmI 2342 GAATGC GAATGC(1/-1)
2598 GAATGC
BspMI 2023 ACCTGC ACCTGC(4/8)
2200 ACCTGC
CelII 41 GCTGAGC GC^T[ACGT]AGC
1902 GCTCAGC
DrdI 2786 GACGCTCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3234 GACTGGCGAGTC
EcoRI 918 GAATTC G^AATTC
2134 GAATTC
EcoRV 1740 GATATC GAT^ATC
2265 GATATC
EcoT22I 2600 ATGCAT ATGCA^T
3299 ATGCAT
HincII 1633 GTCAAC GT[CT]^[GA]AC
2811 GTCGAC
HindII 1633 GTCAAC GT[CT]^[GA]AC
2811 GTCGAC
MluNI 232 TGGCCA TGG^CCA
3049 TGGCCA
MscI 232 TGGCCA TGG^CCA
3049 TGGCCA
MspA1I 1732 CAGCTG C[AC]G^C[GT]G
2216 CAGCGG
NsiI 2600 ATGCAT ATGCA^T
3299 ATGCAT
NspI 53 ACATGC [GA]CATG^[CT]
930 ACATGT
SacI 1383 GAGCTC GAGCT^C
2121 GAGCTC
SbfI 1427 CCTGCAGG CCTGCA^GG
2201 CCTGCAGG
ScaI 291 AGTACT AGT^ACT
1753 AGTACT
Sse8387I 1427 CCTGCAGG CCTGCA^GG
2201 CCTGCAGG
StuI 1153 AGGCCT AGG^CCT
2427 AGGCCT
XbaI 1619 TCTAGA T^CTAGA
3368 TCTAGA
XmnI 401 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3277 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII