##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII BalI BbrPI BbvCI
BcgI BclI BfrI BlpI Bpu1102I
BsaAI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BsrBI BsrFI
BsrGI BssHII BstBI BstEII BstPI
Bsu36I CelII Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
FbaI FseI FspI HindIII HpaI
KpnI MluI MluNI MscI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 1827 GACGTC GACGT^C
AccIII 669 TCCGGA T^CCGGA
AflIII 2831 ACATGT A^C[GA][CT]GT
ApaI 176 GGGCCC GGGCC^C
Asp700I 1800 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 487 GAGCTC G[AT]GC[AT]^C
AvaI 23 CCCGAG C^[CT]CG[GA]G
AvrII 54 CCTAGG C^CTAGG
BanI 201 GGCGCC G^G[CT][GA]CC
BbeI 201 GGCGCC GGCGC^C
BciVI 150 GTATCC GTATCC(6/5)
BglI 263 GCCATGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 54 CCTAGG C^CTAGG
BsaBI 593 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 669 TCCGGA [AT]^CCGG[AT]
BseAI 669 TCCGGA T^CCGGA
BsiHKAI 487 GAGCTC G[AT]GC[AT]^C
BsmFI 2062 GGGAC GGGAC(10/14)
BsoBI 23 CCCGAG C^[CT]CG[GA]G
BspEI 669 TCCGGA T^CCGGA
BspLU11I 2831 ACATGT A^CATGT
BspMI 1235 GCAGGT ACCTGC(4/8)
BssSI 1821 CACGAG CACGAG(-5/-1)
Bst1107I 3117 GTATAC GTA^TAC
BstAPI 2080 GCAAGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3117 GTATAC GTA^TAC
DraII 2024 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1544 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2281 TGGCCG [CT]^GGCC[GA]
EcoO109I 2024 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1802 ATGCAT ATGCA^T
HaeII 201 GGCGCC [GA]GCGC^[CT]
HincII 1869 GTCGAC GT[CT]^[GA]AC
HindII 1869 GTCGAC GT[CT]^[GA]AC
KasI 201 GGCGCC G^GCGCC
KspI 191 CCGCGG CCGC^GG
MamI 593 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1191 CAATTG C^AATTG
MroI 669 TCCGGA T^CCGGA
MunI 1191 CAATTG C^AATTG
NarI 201 GGCGCC GG^CGCC
NcoI 264 CCATGG C^CATGG
NdeI 1230 CATATG CA^TATG
NsiI 1802 ATGCAT ATGCA^T
PpuMI 2024 GGGTCCT [GA]G^G[AT]CC[CT]
SacI 487 GAGCTC GAGCT^C
SacII 191 CCGCGG CCGC^GG
SalI 1869 GTCGAC G^TCGAC
SfoI 201 GGCGCC GGC^GCC
SmlI 2154 CTCAAG C^T[CT][GA]AG
SphI 2598 GCATGC GCATG^C
XbaI 589 TCTAGA T^CTAGA
XcmI 2066 CCACCACCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1800 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BamHI 679 GGATCC G^GATCC
1960 GGATCC
BpmI 628 CTCCAG CTGGAG(16/14)
70 CTGGAG
BstXI 811 CCATGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2069 CCACCACCTTGG
DsaI 191 CCGCGG C^C[GA][CT]GG
264 CCATGG
FauI 205 CCCGC CCCGC(4/6)
101 GCGGG
HphI 1647 TCACC GGTGA(8/7)
1936 TCACC
MslI 1358 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2225 CATGATTATG
NspI 2598 GCATGC [GA]CATG^[CT]
2831 ACATGT
PflMI 1650 CCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1972 CCAGGAAGTGG
PvuII 2537 CAGCTG CAG^CTG
2647 CAGCTG
Van91I 1650 CCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1972 CCAGGAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI AvaII
BanII BbsI BbvI BcnI BfaI
BglII BmyI BpuAI BsaHI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EarI EcoNI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII