##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI Aor51HI ApaI
AscI Asp718I AviII BalI BamHI
BbeI BbrPI BbvCI BciVI BclI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BspEI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I Cfr10I CpoI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
FbaI FseI FspI Hin1I KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccII 341 CGCG CG^CG
AflII 3117 CTTAAG C^TTAAG
AhdI 356 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1378 CCTCAGG CC^T[ACGT]AGG
Asp700I 1307 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 356 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2304 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1231 CCCGAG C^[CT]CG[GA]G
AvrII 877 CCTAGG C^CTAGG
BbsI 2310 GTCTTC GAAGAC(2/6)
BcgI 1401 GCATCGAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3117 CTTAAG C^TTAAG
BglII 2233 AGATCT A^GATCT
BlnI 877 CCTAGG C^CTAGG
BpuAI 2310 GTCTTC GAAGAC(2/6)
BsaAI 1239 CACGTA [CT]AC^GT[GA]
BsaI 2330 GGTCTC GGTCTC(1/5)
BsoBI 1231 CCCGAG C^[CT]CG[GA]G
Bsp1407I 349 TGTACA T^GTACA
BspDI 871 ATCGAT AT^CGAT
BspHI 1167 TCATGA T^CATGA
BsrDI 524 CATTGC GCAATG(2/0)
BsrGI 349 TGTACA T^GTACA
BstAPI 1392 GCAGTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 680 TTCGAA TT^CGAA
BstUI 341 CGCG CG^CG
BstXI 1488 CCAAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2233 AGATCT [GA]^GATC[CT]
Bsu36I 1378 CCTCAGG CC^T[ACGT]AGG
ClaI 871 ATCGAT AT^CGAT
Eam1105I 356 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1378 CCTCAGG CC^T[ACGT]AGG
EcoNI 1954 CCTCTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 847 GATATC GAT^ATC
FauI 92 GCGGG CCCGC(4/6)
HaeII 6 AGCGCC [GA]GCGC^[CT]
HgaI 1548 GCGTC GACGC(5/10)
HincII 1557 GTTAAC GT[CT]^[GA]AC
HindII 1557 GTTAAC GT[CT]^[GA]AC
HindIII 3055 AAGCTT A^AGCTT
HpaI 1557 GTTAAC GTT^AAC
MflI 2233 AGATCT [GA]^GATC[CT]
MvnI 341 CGCG CG^CG
NheI 460 GCTAGC G^CTAGC
NspI 1834 GCATGT [GA]CATG^[CT]
NspV 680 TTCGAA TT^CGAA
PflFI 2304 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1167 TCATGA T^CATGA
SfuI 680 TTCGAA TT^CGAA
SspBI 349 TGTACA T^GTACA
Tth111I 2304 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 2233 AGATCT [GA]^GATC[CT]
XmnI 1307 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 229 GTGCAC G^TGCAC
955 GTGCAC
ApaLI 229 GTGCAC G^TGCAC
955 GTGCAC
AseI 721 ATTAAT AT^TAAT
2959 ATTAAT
AsnI 721 ATTAAT AT^TAAT
2959 ATTAAT
BanII 398 GGGCTC G[GA]GC[CT]^C
2255 GAGCCC
BlpI 1387 GCTCAGC GC^T[ACGT]AGC
1723 GCTCAGC
Bpu1102I 1387 GCTCAGC GC^T[ACGT]AGC
1723 GCTCAGC
BsaBI 1485 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1873 GATAGCTATC
BsgI 445 GTGCAG GTGCAG(16/14)
1115 GTGCAG
BsmFI 920 GGGAC GGGAC(10/14)
2371 GTCCC
BsmI 683 GAATGC GAATGC(1/-1)
3074 GCATTC
CelII 1387 GCTCAGC GC^T[ACGT]AGC
1723 GCTCAGC
CfoI 7 GCGC GCG^C
342 GCGC
DraI 3304 TTTAAA TTT^AAA
3323 TTTAAA
DraII 140 GGGTCCT [GA]G^G[ACGT]CC[CT]
920 GGGACCC
DsaI 1225 CCATGG C^C[GA][CT]GG
2034 CCATGG
EarI 544 GAAGAG CTCTTC(1/4)
738 GAAGAG
EcoO109I 140 GGGTCCT [GA]G^G[ACGT]CC[CT]
920 GGGACCC
EcoRI 833 GAATTC G^AATTC
1784 GAATTC
EcoT22I 1526 ATGCAT ATGCA^T
3072 ATGCAT
HhaI 7 GCGC GCG^C
342 GCGC
HinP1I 7 GCGC G^CGC
342 GCGC
Ksp632I 544 GAAGAG CTCTTC(1/4)
738 GAAGAG
MamI 1485 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1873 GATAGCTATC
NcoI 1225 CCATGG C^CATGG
2034 CCATGG
NsiI 1526 ATGCAT ATGCA^T
3072 ATGCAT
PpuMI 140 GGGTCCT [GA]G^G[AT]CC[CT]
920 GGGACCC
PshBI 721 ATTAAT AT^TAAT
2959 ATTAAT
SmlI 74 CTCAAG C^T[CT][GA]AG
3117 CTTAAG
XbaI 1683 TCTAGA T^CTAGA
3206 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraIII EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I