##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccIII AclI AflIII AgeI
AhdI Alw44I ApaI ApaLI AscI
AseI AsnI Asp718I AspEI BbeI
BbrPI BcgI BciVI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspEI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstZ17I CpoI DraI
DraIII EagI Eam1105I EclXI Eco52I
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI Van91I
XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 290 GACGTC GACGT^C
AccI 272 GTCTAC GT^[AC][GT]AC
AflII 787 CTTAAG C^TTAAG
AocI 2289 CCTCAGG CC^T[ACGT]AGG
AspI 2556 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2433 CCTAGG C^CTAGG
BalI 1293 TGGCCA TGG^CCA
BamHI 2437 GGATCC G^GATCC
BclI 723 TGATCA T^GATCA
BfrI 787 CTTAAG C^TTAAG
BglI 2325 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 999 AGATCT A^GATCT
BlnI 2433 CCTAGG C^CTAGG
BsmBI 2766 CGTCTC CGTCTC(1/5)
BspDI 16 ATCGAT AT^CGAT
BspHI 726 TCATGA T^CATGA
BsrFI 96 ACCGGC [GA]^CCGG[CT]
BssSI 1991 CACGAG CACGAG(-5/-1)
BstAPI 346 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2289 CCTCAGG CC^T[ACGT]AGG
Cfr10I 96 ACCGGC [GA]^CCGG[CT]
ClaI 16 ATCGAT AT^CGAT
DrdI 574 GACACAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2289 CCTCAGG CC^T[ACGT]AGG
EcoRI 985 GAATTC G^AATTC
FbaI 723 TGATCA T^GATCA
MluNI 1293 TGGCCA TGG^CCA
MscI 1293 TGGCCA TGG^CCA
PflFI 2556 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 726 TCATGA T^CATGA
SmaI 2039 CCCGGG CCC^GGG
SspI 27 AATATT AAT^ATT
StuI 2832 AGGCCT AGG^CCT
Tth111I 2556 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2688 TCTAGA T^CTAGA
XmaCI 2039 CCCGGG C^CCGGG
XmaI 2039 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 413 GAATTT [GA]^AATT[CT]
985 GAATTC
AcyI 290 GACGTC G[GA]^CG[CT]C
1821 GGCGTC
Aor51HI 36 AGCGCT AGC^GCT
1527 AGCGCT
ApoI 413 GAATTT [GA]^AATT[CT]
985 GAATTC
Asp700I 202 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2611 GAACAGTTTC
AspHI 1391 GAGCAC G[AT]GC[AT]^C
1901 GTGCTC
AviII 177 TGCGCA TGC^GCA
1227 TGCGCA
BbsI 2464 GAAGAC GAAGAC(2/6)
1892 GTCTTC
BbvCI 1810 CCTCAGC CCTCAGC(-5/-2)
2343 CCTCAGC
BpuAI 2464 GAAGAC GAAGAC(2/6)
1892 GTCTTC
BsaHI 290 GACGTC G[GA]^CG[CT]C
1821 GGCGTC
BsaI 782 GAGACC GGTCTC(1/5)
2270 GAGACC
BseRI 1744 CTCCTC GAGGAG(10/8)
1724 GAGGAG
BsiHKAI 1391 GAGCAC G[AT]GC[AT]^C
1901 GTGCTC
BssHII 1618 GCGCGC G^CGCGC
1847 GCGCGC
BstEII 2175 GGTCACC G^GT[ACGT]ACC
2364 GGTGACC
BstPI 2175 GGTCACC G^GT[ACGT]ACC
2364 GGTGACC
BstXI 1164 CCAAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1836 CCAACAAGATGG
Eco47III 36 AGCGCT AGC^GCT
1527 AGCGCT
EcoNI 1330 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1474 CCTCCCAGAGG
EcoO65I 2175 GGTCACC G^GT[ACGT]ACC
2364 GGTGACC
FspI 177 TGCGCA TGC^GCA
1227 TGCGCA
Hin1I 290 GACGTC G[GA]^CG[CT]C
1821 GGCGTC
MslI 868 CATAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1991 CACGAGAATG
NcoI 1119 CCATGG C^CATGG
2127 CCATGG
XcmI 588 CCACCTCAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1806 CCACCCTCAGCATGG
XmnI 202 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2611 GAACAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII