##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AviII       BamHI       BbeI        BbrPI       BcgI        
BclI        BfrI        BglII       BsaAI       BsaBI       
BsaHI       BseAI       BsiEI       BsiWI       BspDI       
BspEI       BsrDI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     CfoI        ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     FbaI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
MamI        MfeI        MluI        MroI        MunI        
NarI        NdeI        NotI        NruI        NspV        
PacI        PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RsrII       SacI        SalI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SspI        
SwaI        XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         2823  GTAGAC               GT^[AC][GT]AC
AccII        1340  CGCG                 CG^CG
AcsI         1480  GAATTC               [GA]^AATT[CT]
AflIII        666  ACATGT               A^C[GA][CT]GT
AgeI         2658  ACCGGT               A^CCGGT
ApoI         1480  GAATTC               [GA]^AATT[CT]
Asp700I       959  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1541  GAGCAC               G[AT]GC[AT]^C
BalI          903  TGGCCA               TGG^CCA
BciVI        2954  GTATCC               GTATCC(6/5)
BsiHKAI      1541  GAGCAC               G[AT]GC[AT]^C
BsmBI         559  GAGACG               CGTCTC(1/5)
Bsp1407I     1300  TGTACA               T^GTACA
BspHI        2029  TCATGA               T^CATGA
BspLU11I      666  ACATGT               A^CATGT
BsrBI         764  GAGCGG               CCGCTC(-3/-3)
BsrGI        1300  TGTACA               T^GTACA
BssSI        2703  CACGAG               CACGAG(-5/-1)
BstAPI       1072  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1340  CGCG                 CG^CG
DraIII       1158  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         359  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1480  GAATTC               G^AATTC
EcoRV        2380  GATATC               GAT^ATC
EcoT22I      2478  ATGCAT               ATGCA^T
FseI          577  GGCCGGCC             GGCCGG^CC
HgaI          259  GACGC                GACGC(5/10)
KspI         1339  CCGCGG               CCGC^GG
MaeII         537  ACGT                 A^CGT
MluNI         903  TGGCCA               TGG^CCA
MscI          903  TGGCCA               TGG^CCA
MvnI         1340  CGCG                 CG^CG
NaeI          578  GCCGGC               GCC^GGC
NgoMIV        578  GCCGGC               G^CCGGC
NheI         1897  GCTAGC               G^CTAGC
NsiI         2478  ATGCAT               ATGCA^T
PaeR7I       2691  CTCGAG               C^TCGAG
PinAI        2658  ACCGGT               A^CCGGT
PshAI         310  GACTGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         2029  TCATGA               T^CATGA
SacII        1339  CCGCGG               CCGC^GG
SphI         2480  GCATGC               GCATG^C
SspBI        1300  TGTACA               T^GTACA
TaqI         2692  TCGA                 T^CGA
TthHB8I      2692  TCGA                 T^CGA
XhoI         2691  CTCGAG               C^TCGAG
XmnI          959  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AocI           60  CCTCAGG              CC^T[ACGT]AGG
             3093  CCTCAGG             
AvaI          785  CCCGAG               C^[CT]CG[GA]G
             2691  CTCGAG              
AvrII        2749  CCTAGG               C^CTAGG
             2992  CCTAGG              
BbsI           18  GAAGAC               GAAGAC(2/6)
              885  GTCTTC              
BlnI         2749  CCTAGG               C^CTAGG
             2992  CCTAGG              
BlpI         1367  GCTGAGC              GC^T[ACGT]AGC
             1538  GCTGAGC             
Bpu1102I     1367  GCTGAGC              GC^T[ACGT]AGC
             1538  GCTGAGC             
BpuAI          18  GAAGAC               GAAGAC(2/6)
              885  GTCTTC              
BsaWI        1530  ACCGGA               [AT]^CCGG[AT]
             2658  ACCGGT              
BsgI         2415  CTGCAC               GTGCAG(16/14)
              636  GTGCAG              
BsmI         1006  GAATGC               GAATGC(1/-1)
              294  GCATTC              
BsoBI         785  CCCGAG               C^[CT]CG[GA]G
             2691  CTCGAG              
BsrFI         578  GCCGGC               [GA]^CCGG[CT]
             2658  ACCGGT              
Bsu36I         60  CCTCAGG              CC^T[ACGT]AGG
             3093  CCTCAGG             
CelII        1367  GCTGAGC              GC^T[ACGT]AGC
             1538  GCTGAGC             
Cfr10I        578  GCCGGC               [GA]^CCGG[CT]
             2658  ACCGGT              
DrdI          371  GACACAGTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1424  GACCCTGGGGTC        
EaeI          903  TGGCCA               [CT]^GGCC[GA]
             1570  TGGCCG              
Eco81I         60  CCTCAGG              CC^T[ACGT]AGG
             3093  CCTCAGG             
MslI          403  CACCGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              532  CACCGACGTG          
SapI          959  GAAGAGC              GCTCTTC(1/4)
              262  GCTCTTC             
SbfI          190  CCTGCAGG             CCTGCA^GG
              391  CCTGCAGG            
Sse8387I      190  CCTGCAGG             CCTGCA^GG
              391  CCTGCAGG            


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
ApaI        AspI        AvaII       BanI        BanII       
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       DsaI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflFI       
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     Van91I      XcmI        XhoII