##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII BamHI BbeI BbrPI BcgI
BclI BfrI BglII BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BspDI
BspEI BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CfoI ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FbaI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MluI MroI MunI
NarI NdeI NotI NruI NspV
PacI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SspI
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 2823 GTAGAC GT^[AC][GT]AC
AccII 1340 CGCG CG^CG
AcsI 1480 GAATTC [GA]^AATT[CT]
AflIII 666 ACATGT A^C[GA][CT]GT
AgeI 2658 ACCGGT A^CCGGT
ApoI 1480 GAATTC [GA]^AATT[CT]
Asp700I 959 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1541 GAGCAC G[AT]GC[AT]^C
BalI 903 TGGCCA TGG^CCA
BciVI 2954 GTATCC GTATCC(6/5)
BsiHKAI 1541 GAGCAC G[AT]GC[AT]^C
BsmBI 559 GAGACG CGTCTC(1/5)
Bsp1407I 1300 TGTACA T^GTACA
BspHI 2029 TCATGA T^CATGA
BspLU11I 666 ACATGT A^CATGT
BsrBI 764 GAGCGG CCGCTC(-3/-3)
BsrGI 1300 TGTACA T^GTACA
BssSI 2703 CACGAG CACGAG(-5/-1)
BstAPI 1072 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1340 CGCG CG^CG
DraIII 1158 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 359 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1480 GAATTC G^AATTC
EcoRV 2380 GATATC GAT^ATC
EcoT22I 2478 ATGCAT ATGCA^T
FseI 577 GGCCGGCC GGCCGG^CC
HgaI 259 GACGC GACGC(5/10)
KspI 1339 CCGCGG CCGC^GG
MaeII 537 ACGT A^CGT
MluNI 903 TGGCCA TGG^CCA
MscI 903 TGGCCA TGG^CCA
MvnI 1340 CGCG CG^CG
NaeI 578 GCCGGC GCC^GGC
NgoMIV 578 GCCGGC G^CCGGC
NheI 1897 GCTAGC G^CTAGC
NsiI 2478 ATGCAT ATGCA^T
PaeR7I 2691 CTCGAG C^TCGAG
PinAI 2658 ACCGGT A^CCGGT
PshAI 310 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2029 TCATGA T^CATGA
SacII 1339 CCGCGG CCGC^GG
SphI 2480 GCATGC GCATG^C
SspBI 1300 TGTACA T^GTACA
TaqI 2692 TCGA T^CGA
TthHB8I 2692 TCGA T^CGA
XhoI 2691 CTCGAG C^TCGAG
XmnI 959 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 60 CCTCAGG CC^T[ACGT]AGG
3093 CCTCAGG
AvaI 785 CCCGAG C^[CT]CG[GA]G
2691 CTCGAG
AvrII 2749 CCTAGG C^CTAGG
2992 CCTAGG
BbsI 18 GAAGAC GAAGAC(2/6)
885 GTCTTC
BlnI 2749 CCTAGG C^CTAGG
2992 CCTAGG
BlpI 1367 GCTGAGC GC^T[ACGT]AGC
1538 GCTGAGC
Bpu1102I 1367 GCTGAGC GC^T[ACGT]AGC
1538 GCTGAGC
BpuAI 18 GAAGAC GAAGAC(2/6)
885 GTCTTC
BsaWI 1530 ACCGGA [AT]^CCGG[AT]
2658 ACCGGT
BsgI 2415 CTGCAC GTGCAG(16/14)
636 GTGCAG
BsmI 1006 GAATGC GAATGC(1/-1)
294 GCATTC
BsoBI 785 CCCGAG C^[CT]CG[GA]G
2691 CTCGAG
BsrFI 578 GCCGGC [GA]^CCGG[CT]
2658 ACCGGT
Bsu36I 60 CCTCAGG CC^T[ACGT]AGG
3093 CCTCAGG
CelII 1367 GCTGAGC GC^T[ACGT]AGC
1538 GCTGAGC
Cfr10I 578 GCCGGC [GA]^CCGG[CT]
2658 ACCGGT
DrdI 371 GACACAGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1424 GACCCTGGGGTC
EaeI 903 TGGCCA [CT]^GGCC[GA]
1570 TGGCCG
Eco81I 60 CCTCAGG CC^T[ACGT]AGG
3093 CCTCAGG
MslI 403 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
532 CACCGACGTG
SapI 959 GAAGAGC GCTCTTC(1/4)
262 GCTCTTC
SbfI 190 CCTGCAGG CCTGCA^GG
391 CCTGCAGG
Sse8387I 190 CCTGCAGG CCTGCA^GG
391 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I Van91I XcmI XhoII