##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccIII AclI AflII Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspI AviII BbrPI BbsI
BcgI BciVI BclI BfrI BpuAI
BsaBI BseAI BsiWI BspDI BspEI
BspHI BspLU11I BssHII BstBI ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoRI EcoT22I
FbaI FspI HincII HindII HindIII
HpaI MamI MluI MroI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 2513 GGTACC G^GTACC
AgeI 1830 ACCGGT A^CCGGT
AhdI 1535 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 105 CCTGAGG CC^T[ACGT]AGG
Asp718I 2513 GGTACC G^GTACC
AspEI 1535 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2306 TGGCCA TGG^CCA
BamHI 89 GGATCC G^GATCC
BglII 2455 AGATCT A^GATCT
BlpI 883 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 883 GCTTAGC GC^T[ACGT]AGC
BsaWI 1830 ACCGGT [AT]^CCGG[AT]
BsiEI 118 CGACCG CG[GA][CT]^CG
BsmBI 2483 CGTCTC CGTCTC(1/5)
BsmI 916 GAATGC GAATGC(1/-1)
Bsp1407I 1124 TGTACA T^GTACA
BsrDI 2941 GCAATG GCAATG(2/0)
BsrGI 1124 TGTACA T^GTACA
BstAPI 655 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2611 CCAGCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 105 CCTGAGG CC^T[ACGT]AGG
CelII 883 GCTTAGC GC^T[ACGT]AGC
DraIII 1797 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1535 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 105 CCTGAGG CC^T[ACGT]AGG
EcoRV 2341 GATATC GAT^ATC
FseI 2414 GGCCGGCC GGCCGG^CC
KpnI 2513 GGTACC GGTAC^C
MfeI 3042 CAATTG C^AATTG
MluNI 2306 TGGCCA TGG^CCA
MscI 2306 TGGCCA TGG^CCA
MunI 3042 CAATTG C^AATTG
PinAI 1830 ACCGGT A^CCGGT
SspBI 1124 TGTACA T^GTACA
TaqI 1719 TCGA T^CGA
TthHB8I 1719 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 1524 ACGTGT A^C[GA][CT]GT
2929 ACGTGT
AlwI 90 GATCC GGATC(4/5)
89 GGATC
AvrII 272 CCTAGG C^CTAGG
2570 CCTAGG
BlnI 272 CCTAGG C^CTAGG
2570 CCTAGG
BsaAI 1360 CACGTA [CT]AC^GT[GA]
2199 TACGTG
BsaI 1542 GGTCTC GGTCTC(1/5)
2716 GGTCTC
BsgI 1228 CTGCAC GTGCAG(16/14)
2268 CTGCAC
Bst1107I 1324 GTATAC GTA^TAC
2677 GTATAC
BstEII 706 GGTCACC G^GT[ACGT]ACC
1565 GGTGACC
BstPI 706 GGTCACC G^GT[ACGT]ACC
1565 GGTGACC
BstYI 89 GGATCC [GA]^GATC[CT]
2455 AGATCT
BstZ17I 1324 GTATAC GTA^TAC
2677 GTATAC
EcoO65I 706 GGTCACC G^GT[ACGT]ACC
1565 GGTGACC
KspI 61 CCGCGG CCGC^GG
364 CCGCGG
MflI 89 GGATCC [GA]^GATC[CT]
2455 AGATCT
MslI 697 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
835 CACTCTAGTG
NcoI 1374 CCATGG C^CATGG
1530 CCATGG
SacI 2376 GAGCTC GAGCT^C
2461 GAGCTC
SacII 61 CCGCGG CCGC^GG
364 CCGCGG
SapI 1081 GCTCTTC GCTCTTC(1/4)
2469 GCTCTTC
SfiI 1703 GGCCTGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2414 GGCCGGCCTGGCC
SmaI 318 CCCGGG CCC^GGG
1893 CCCGGG
SmlI 1070 CTCAAG C^T[CT][GA]AG
2102 CTTGAG
XcmI 437 CCAGCCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1263 CCATGCTGGACCTGG
XhoII 89 GGATCC [GA]^GATC[CT]
2455 AGATCT
XmaCI 318 CCCGGG C^CCGGG
1893 CCCGGG
XmaI 318 CCCGGG C^CCGGG
1893 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwNI ApaI ApoI
AspHI AvaI AvaII BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XbaI