##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspI BalI BciVI
BclI BfrI BlpI Bpu1102I BsaBI
BsiWI BsmBI BspDI BssHII Bst1107I
BstBI BstXI BstZ17I CelII ClaI
CpoI Eco47III EcoRV EcoT22I FbaI
FseI HpaI KpnI KspI MamI
MfeI MluI MluNI MscI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 158 CGCG CG^CG
AccIII 622 TCCGGA T^CCGGA
AflIII 2945 ACATGT A^C[GA][CT]GT
AgeI 1323 ACCGGT A^CCGGT
AhdI 687 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 687 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 528 TGCGCA TGC^GCA
BbrPI 2899 CACGTG CAC^GTG
BbvCI 481 CCTCAGC CCTCAGC(-5/-2)
BcgI 1413 CGAAGGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 2899 CACGTG [CT]AC^GT[GA]
BseAI 622 TCCGGA T^CCGGA
Bsp1407I 978 TGTACA T^GTACA
BspEI 622 TCCGGA T^CCGGA
BspLU11I 2945 ACATGT A^CATGT
BspMI 3010 GCAGGT ACCTGC(4/8)
BsrDI 974 GCAATG GCAATG(2/0)
BsrGI 978 TGTACA T^GTACA
BssSI 756 CACGAG CACGAG(-5/-1)
BstUI 158 CGCG CG^CG
DraI 2639 TTTAAA TTT^AAA
DraIII 2896 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 304 GACGATGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 154 CGGCCG [CT]^GGCC[GA]
EagI 154 CGGCCG C^GGCCG
Eam1105I 687 GACAACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 154 CGGCCG C^GGCCG
Eco52I 154 CGGCCG C^GGCCG
FspI 528 TGCGCA TGC^GCA
HgaI 311 GCGTC GACGC(5/10)
HincII 2818 GTCAAC GT[CT]^[GA]AC
HindII 2818 GTCAAC GT[CT]^[GA]AC
MroI 622 TCCGGA T^CCGGA
MvnI 158 CGCG CG^CG
PinAI 1323 ACCGGT A^CCGGT
PmaCI 2899 CACGTG CAC^GTG
PmlI 2899 CACGTG CAC^GTG
SmlI 1387 CTCAAG C^T[CT][GA]AG
SphI 2067 GCATGC GCATG^C
SspBI 978 TGTACA T^GTACA
XcmI 454 CCAAGAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AocI 1253 CCTCAGG CC^T[ACGT]AGG
2576 CCTCAGG
AspHI 1584 GAGCAC G[AT]GC[AT]^C
2617 GTGCTC
AvrII 139 CCTAGG C^CTAGG
3185 CCTAGG
BamHI 1009 GGATCC G^GATCC
1138 GGATCC
BbsI 923 GAAGAC GAAGAC(2/6)
1311 GAAGAC
BglII 543 AGATCT A^GATCT
1797 AGATCT
BlnI 139 CCTAGG C^CTAGG
3185 CCTAGG
BpuAI 923 GAAGAC GAAGAC(2/6)
1311 GAAGAC
BsaWI 622 TCCGGA [AT]^CCGG[AT]
1323 ACCGGT
BsiHKAI 1584 GAGCAC G[AT]GC[AT]^C
2617 GTGCTC
BsmI 2459 GCATTC GAATGC(1/-1)
3140 GCATTC
BsrBI 171 GAGCGG CCGCTC(-3/-3)
628 GAGCGG
BstEII 643 GGTGACC G^GT[ACGT]ACC
2571 GGTGACC
BstPI 643 GGTGACC G^GT[ACGT]ACC
2571 GGTGACC
Bsu36I 1253 CCTCAGG CC^T[ACGT]AGG
2576 CCTCAGG
EarI 1196 GAAGAG CTCTTC(1/4)
2782 GAAGAG
Eco81I 1253 CCTCAGG CC^T[ACGT]AGG
2576 CCTCAGG
EcoO65I 643 GGTGACC G^GT[ACGT]ACC
2571 GGTGACC
EcoRI 1836 GAATTC G^AATTC
2586 GAATTC
HindIII 2053 AAGCTT A^AGCTT
2305 AAGCTT
Ksp632I 1196 GAAGAG CTCTTC(1/4)
2782 GAAGAG
PflMI 1352 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3020 CCAGGAATTGG
PstI 818 CTGCAG CTGCA^G
1421 CTGCAG
Van91I 1352 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3020 CCAGGAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApaI ApoI Asp700I
AvaI AvaII BanI BanII BbeI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmaCI XmaI XmnI