##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AvaI AviII AvrII BamHI
BbeI BcgI BclI BcnI BglI
BlnI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmAI BsmBI BsoBI BspDI BspEI
BsrBI BsrFI BssHII Bst1107I BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI FbaI FseI FspI HaeII
HapII HgaI Hin1I HpaII KasI
KpnI KspI MfeI MluI MroI
MspA1I MspI MunI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI PacI PaeR7I PinAI PmeI
PshAI PvuI PvuII RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AclI 512 AACGTT AA^CGTT
AflII 1974 CTTAAG C^TTAAG
AseI 3260 ATTAAT AT^TAAT
AsnI 3260 ATTAAT AT^TAAT
AspHI 1638 GAGCTC G[AT]GC[AT]^C
AspI 2213 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1464 TGGCCA TGG^CCA
BanII 1638 GAGCTC G[GA]GC[CT]^C
BbrPI 21 CACGTG CAC^GTG
BbvCI 978 CCTCAGC CCTCAGC(-5/-2)
BbvI 2052 GCAGC GCAGC(8/12)
BciVI 2871 GGATAC GTATCC(6/5)
BfrI 1974 CTTAAG C^TTAAG
BmyI 1638 GAGCTC G[AGT]GC[ACT]^C
BsaAI 21 CACGTG [CT]AC^GT[GA]
BsaBI 678 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1776 GTGCAG GTGCAG(16/14)
BsiHKAI 1638 GAGCTC G[AT]GC[AT]^C
Bsp1286I 1638 GAGCTC G[AGT]GC[ACT]^C
BspHI 397 TCATGA T^CATGA
BspMI 2048 ACCTGC ACCTGC(4/8)
BsrDI 1610 GCAATG GCAATG(2/0)
BssSI 1083 CACGAG CACGAG(-5/-1)
BstAPI 619 GCAAGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1183 TTCGAA TT^CGAA
BstEII 2896 GGTCACC G^GT[ACGT]ACC
BstPI 2896 GGTCACC G^GT[ACGT]ACC
BstXI 2136 CCATAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 983 GCCAGC GC[ACGT]^[ACGT]GC
DraII 106 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 1464 TGGCCA [CT]^GGCC[GA]
EcoO109I 106 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2896 GGTCACC G^GT[ACGT]ACC
EcoRV 3058 GATATC GAT^ATC
FauI 1283 GCGGG CCCGC(4/6)
Fnu4HI 2052 GCAGC GC^[ACGT]GC
ItaI 2052 GCAGC GC^[ACGT]GC
MamI 678 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1464 TGGCCA TGG^CCA
MscI 1464 TGGCCA TGG^CCA
NspV 1183 TTCGAA TT^CGAA
PflFI 2213 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 242 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 2240 GACTC GAGTC(4/5)
PmaCI 21 CACGTG CAC^GTG
PmlI 21 CACGTG CAC^GTG
PpuMI 106 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 3260 ATTAAT AT^TAAT
Psp1406I 512 AACGTT AA^CGTT
PstI 2050 CTGCAG CTGCA^G
RcaI 397 TCATGA T^CATGA
SacI 1638 GAGCTC GAGCT^C
SfcI 2050 CTGCAG C^T[GA][CT]AG
SfuI 1183 TTCGAA TT^CGAA
SpeI 722 ACTAGT A^CTAGT
StuI 2113 AGGCCT AGG^CCT
TseI 2052 GCAGC G^C[AT]GC
Tth111I 2213 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 242 CCAGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 765 TCTAGA T^CTAGA
XcmI 2133 CCACCATAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 28 CGCG CG^CG
183 CGCG
AvaII 17 GGTCC G^G[AT]CC
107 GGACC
BanI 221 GGCACC G^G[CT][GA]CC
238 GGCACC
BglII 2140 AGATCT A^GATCT
2148 AGATCT
Bsp1407I 1569 TGTACA T^GTACA
2844 TGTACA
BsrGI 1569 TGTACA T^GTACA
2844 TGTACA
BstUI 28 CGCG CG^CG
183 CGCG
CfoI 27 GCGC GCG^C
182 GCGC
HhaI 27 GCGC GCG^C
182 GCGC
HinP1I 27 GCGC G^CGC
182 GCGC
HincII 74 GTTAAC GT[CT]^[GA]AC
1950 GTTAAC
HindII 74 GTTAAC GT[CT]^[GA]AC
1950 GTTAAC
HpaI 74 GTTAAC GTT^AAC
1950 GTTAAC
MvnI 28 CGCG CG^CG
183 CGCG
MwoI 184 GCGGAAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
619 GCAAGTCATGC
ScaI 1963 AGTACT AGT^ACT
2810 AGTACT
SmlI 231 CTTGAG C^T[CT][GA]AG
1974 CTTAAG
SspBI 1569 TGTACA T^GTACA
2844 TGTACA
TaqI 611 TCGA T^CGA
1184 TCGA
TthHB8I 611 TCGA T^CGA
1184 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I BbsI BfaI
BpmI BpuAI BsaJI BsiYI BslI
BsmFI BsmI BspLU11I BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
EcoT22I FokI HaeIII HindIII HinfI
HphI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI NdeI NdeII NlaIII
NlaIV NsiI NspI RsaI Sau3AI
Sau96I ScrFI SfaNI SspI StyI
TfiI Tru9I Tsp45I Tsp509I TspRI
XhoII XmnI