##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AgeI AhdI Alw44I
ApaLI AseI AsnI Asp700I Asp718I
AspEI AviII BamHI BbeI BbrPI
BsaAI BsaBI BsiWI BspDI BspHI
BsrBI BsrDI BssSI BstBI ClaI
CpoI DraI DraIII DrdI Eam1105I
EcoRV EcoT22I FspI HincII HindII
HpaI KasI KpnI MamI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Van91I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 3191 GTATAC GT^[AC][GT]AC
AccIII 240 TCCGGA T^CCGGA
AclI 1533 AACGTT AA^CGTT
AflII 2610 CTTAAG C^TTAAG
AflIII 2865 ACATGT A^C[GA][CT]GT
AocI 799 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1319 AGCGCT AGC^GCT
AscI 687 GGCGCGCC GG^CGCGCC
AspI 2179 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 393 GTCTTC GAAGAC(2/6)
BbvCI 2561 CCTCAGC CCTCAGC(-5/-2)
BcgI 443 CGAGGGTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1942 GTATCC GTATCC(6/5)
BfrI 2610 CTTAAG C^TTAAG
BglII 1103 AGATCT A^GATCT
BpuAI 393 GTCTTC GAAGAC(2/6)
BsaWI 240 TCCGGA [AT]^CCGG[AT]
BseAI 240 TCCGGA T^CCGGA
BsmBI 576 CGTCTC CGTCTC(1/5)
Bsp1407I 1022 TGTACA T^GTACA
BspEI 240 TCCGGA T^CCGGA
BspLU11I 2865 ACATGT A^CATGT
BsrGI 1022 TGTACA T^GTACA
BssHII 688 GCGCGC G^CGCGC
Bst1107I 3191 GTATAC GTA^TAC
BstEII 2830 GGTTACC G^GT[ACGT]ACC
BstPI 2830 GGTTACC G^GT[ACGT]ACC
BstZ17I 3191 GTATAC GTA^TAC
Bsu36I 799 CCTGAGG CC^T[ACGT]AGG
Eco47III 1319 AGCGCT AGC^GCT
Eco81I 799 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2830 GGTTACC G^GT[ACGT]ACC
EcoRI 1385 GAATTC G^AATTC
FseI 197 GGCCGGCC GGCCGG^CC
HindIII 2952 AAGCTT A^AGCTT
KspI 521 CCGCGG CCGC^GG
MroI 240 TCCGGA T^CCGGA
NspI 2865 ACATGT [GA]CATG^[CT]
PaeR7I 164 CTCGAG C^TCGAG
PflFI 2179 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1533 AACGTT AA^CGTT
SacI 304 GAGCTC GAGCT^C
SacII 521 CCGCGG CCGC^GG
SfiI 68 GGCCCGACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 93 CCCGGG CCC^GGG
SspBI 1022 TGTACA T^GTACA
Tsp45I 1238 GTGAC ^GT[GC]AC
Tth111I 2179 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 164 CTCGAG C^TCGAG
XmaCI 93 CCCGGG C^CCGGG
XmaI 93 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 103 GGCGTC G[GA]^CG[CT]C
574 GGCGTC
AspHI 304 GAGCTC G[AT]GC[AT]^C
634 GTGCTC
AvrII 2189 CCTAGG C^CTAGG
2651 CCTAGG
BalI 1040 TGGCCA TGG^CCA
1457 TGGCCA
BclI 1834 TGATCA T^GATCA
1965 TGATCA
BlnI 2189 CCTAGG C^CTAGG
2651 CCTAGG
BsaHI 103 GGCGTC G[GA]^CG[CT]C
574 GGCGTC
BsiHKAI 304 GAGCTC G[AT]GC[AT]^C
634 GTGCTC
BsmI 1563 GAATGC GAATGC(1/-1)
2582 GCATTC
BstAPI 462 GCAGCTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1064 GCAACTCCTGC
BstYI 1103 AGATCT [GA]^GATC[CT]
1412 AGATCC
EagI 119 CGGCCG C^GGCCG
200 CGGCCG
EclXI 119 CGGCCG C^GGCCG
200 CGGCCG
Eco52I 119 CGGCCG C^GGCCG
200 CGGCCG
FbaI 1834 TGATCA T^GATCA
1965 TGATCA
HaeII 852 GGCGCT [GA]GCGC^[CT]
1319 AGCGCT
Hin1I 103 GGCGTC G[GA]^CG[CT]C
574 GGCGTC
MaeII 278 ACGT A^CGT
1534 ACGT
MflI 1103 AGATCT [GA]^GATC[CT]
1412 AGATCC
MluNI 1040 TGGCCA TGG^CCA
1457 TGGCCA
MscI 1040 TGGCCA TGG^CCA
1457 TGGCCA
MslI 561 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1677 CATTCTGATG
StuI 1277 AGGCCT AGG^CCT
1839 AGGCCT
XcmI 839 CCAACAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1625 CCAGCGACCACCTGG
XhoII 1103 AGATCT [GA]^GATC[CT]
1412 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I