##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AclI AflII AocI
Aor51HI AscI AseI AsnI Asp718I
BamHI BbrPI BciVI BfrI BlpI
Bpu1102I BsaAI BsaBI BsiWI BsmI
BspDI BspLU11I BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DrdI Eco47III Eco81I
EcoO65I EcoRI EcoRV FseI HindIII
HpaI KpnI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SfuI
SgrAI SmlI SnaBI SpeI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccIII 213 TCCGGA T^CCGGA
AcsI 1670 AAATTC [GA]^AATT[CT]
AflIII 1167 ACGTGT A^C[GA][CT]GT
AgeI 339 ACCGGT A^CCGGT
AhdI 2477 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2538 GGGCCC GGGCC^C
ApoI 1670 AAATTC [GA]^AATT[CT]
Asp700I 1156 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2477 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1249 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2976 CCTAGG C^CTAGG
BbeI 1724 GGCGCC GGCGC^C
BcgI 828 GCACAGTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 957 TGATCA T^GATCA
BglII 2609 AGATCT A^GATCT
BlnI 2976 CCTAGG C^CTAGG
BsaI 2870 GAGACC GGTCTC(1/5)
BseAI 213 TCCGGA T^CCGGA
BsiEI 1836 CGGCCG CG[GA][CT]^CG
BsmBI 350 CGTCTC CGTCTC(1/5)
BspEI 213 TCCGGA T^CCGGA
BspHI 1929 TCATGA T^CATGA
BsrBI 902 GAGCGG CCGCTC(-3/-3)
BssHII 1829 GCGCGC G^CGCGC
DraIII 2851 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1836 CGGCCG C^GGCCG
Eam1105I 2477 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1836 CGGCCG C^GGCCG
Eco52I 1836 CGGCCG C^GGCCG
EcoT22I 2984 ATGCAT ATGCA^T
FbaI 957 TGATCA T^GATCA
KasI 1724 GGCGCC G^GCGCC
MroI 213 TCCGGA T^CCGGA
NarI 1724 GGCGCC GG^CGCC
NsiI 2984 ATGCAT ATGCA^T
PflFI 1249 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2074 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 339 ACCGGT A^CCGGT
PpuMI 567 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 3032 CAGCTG CAG^CTG
RcaI 1929 TCATGA T^CATGA
SacI 2398 GAGCTC GAGCT^C
SapI 2113 GCTCTTC GCTCTTC(1/4)
SbfI 124 CCTGCAGG CCTGCA^GG
ScaI 1029 AGTACT AGT^ACT
SfiI 2539 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1724 GGCGCC GGC^GCC
SmaI 2737 CCCGGG CCC^GGG
SphI 693 GCATGC GCATG^C
Sse8387I 124 CCTGCAGG CCTGCA^GG
Tth111I 1249 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2074 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2737 CCCGGG C^CCGGG
XmaI 2737 CCCGGG C^CCGGG
XmnI 1156 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 1830 CGCG CG^CG
2086 CGCG
Alw44I 473 GTGCAC G^TGCAC
2848 GTGCAC
ApaLI 473 GTGCAC G^TGCAC
2848 GTGCAC
AviII 1785 TGCGCA TGC^GCA
2130 TGCGCA
BalI 45 TGGCCA TGG^CCA
976 TGGCCA
BbsI 1061 GAAGAC GAAGAC(2/6)
1916 GTCTTC
BbvCI 1752 CCTCAGC CCTCAGC(-5/-2)
1679 GCTGAGG
BglI 1963 GCCCTTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2540 GCCCTGGAGGC
BpuAI 1061 GAAGAC GAAGAC(2/6)
1916 GTCTTC
BsaWI 213 TCCGGA [AT]^CCGG[AT]
339 ACCGGT
Bsp1407I 1806 TGTACA T^GTACA
1977 TGTACA
BsrGI 1806 TGTACA T^GTACA
1977 TGTACA
BstAPI 127 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2850 GCACCTGGTGC
BstUI 1830 CGCG CG^CG
2086 CGCG
DraI 2654 TTTAAA TTT^AAA
3135 TTTAAA
EcoNI 211 CCTCCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2710 CCTCAGTGAGG
FspI 1785 TGCGCA TGC^GCA
2130 TGCGCA
HaeII 101 AGCGCC [GA]GCGC^[CT]
1724 GGCGCC
HincII 374 GTTGAC GT[CT]^[GA]AC
554 GTCAAC
HindII 374 GTTGAC GT[CT]^[GA]AC
554 GTCAAC
MluNI 45 TGGCCA TGG^CCA
976 TGGCCA
MscI 45 TGGCCA TGG^CCA
976 TGGCCA
MvnI 1830 CGCG CG^CG
2086 CGCG
NaeI 2032 GCCGGC GCC^GGC
2731 GCCGGC
NcoI 1398 CCATGG C^CATGG
1773 CCATGG
NgoMIV 2032 GCCGGC G^CCGGC
2731 GCCGGC
NspI 693 GCATGC [GA]CATG^[CT]
1980 ACATGC
SspBI 1806 TGTACA T^GTACA
1977 TGTACA
StuI 1795 AGGCCT AGG^CCT
1869 AGGCCT
TfiI 765 GAATC G^A[AT]TC
2515 GATTC
Tsp509I 1671 AATT ^AATT
2601 AATT
XcmI 385 CCAAAGGGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2206 CCACGTCTGCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I XhoII