##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AocI AscI AseI AsnI Asp700I
Asp718I AvrII BfrI BlnI BsaI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BsrDI BsrGI BssHII BstBI
Bsu36I ClaI CpoI DraI Eco81I
EcoRI EcoRV EcoT22I FseI HindIII
KpnI MfeI MluI MroI MunI
NdeI NheI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SfuI SnaBI
SpeI SspBI SspI SwaI TfiI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 1901 GAATTT [GA]^AATT[CT]
AflIII 960 ACATGT A^C[GA][CT]GT
Alw44I 1274 GTGCAC G^TGCAC
ApaLI 1274 GTGCAC G^TGCAC
ApoI 1901 GAATTT [GA]^AATT[CT]
AspI 1871 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1304 GTCTTC GAAGAC(2/6)
BcgI 2329 GCAGCCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2134 GGATAC GTATCC(6/5)
BclI 1881 TGATCA T^GATCA
BpuAI 1304 GTCTTC GAAGAC(2/6)
BsaBI 277 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 2936 GTCTC GTCTC(1/5)
BsmBI 2935 CGTCTC CGTCTC(1/5)
BspHI 1878 TCATGA T^CATGA
BspLU11I 960 ACATGT A^CATGT
BsrBI 2691 CCGCTC CCGCTC(-3/-3)
Bst1107I 2119 GTATAC GTA^TAC
BstZ17I 2119 GTATAC GTA^TAC
DrdI 1256 GACGGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1881 TGATCA T^GATCA
HpaI 2719 GTTAAC GTT^AAC
MamI 277 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 723 GCGGCCGC GC^GGCCGC
NruI 2194 TCGCGA TCG^CGA
PflFI 1871 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1878 TCATGA T^CATGA
SacI 451 GAGCTC GAGCT^C
SalI 1862 GTCGAC G^TCGAC
SbfI 2735 CCTGCAGG CCTGCA^GG
ScaI 1530 AGTACT AGT^ACT
SexAI 2489 ACCAGGT A^CC[AT]GGT
SfiI 1675 GGCCATGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 754 CACCGGCG C[GA]^CCGG[CT]G
SmaI 954 CCCGGG CCC^GGG
SphI 2052 GCATGC GCATG^C
Sse8387I 2735 CCTGCAGG CCTGCA^GG
Tth111I 1871 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 954 CCCGGG C^CCGGG
XmaI 954 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1862 GTCGAC GT^[AC][GT]AC
2119 GTATAC
AhdI 1139 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2144 GACAGCATGTC
Aor51HI 2556 AGCGCT AGC^GCT
2968 AGCGCT
AspEI 1139 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2144 GACAGCATGTC
AviII 246 TGCGCA TGC^GCA
1026 TGCGCA
BamHI 1113 GGATCC G^GATCC
1668 GGATCC
BbrPI 530 CACGTG CAC^GTG
1574 CACGTG
BfaI 23 CTAG C^TAG
467 CTAG
BglI 676 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1676 GCCATGAAGGC
BglII 1065 AGATCT A^GATCT
1839 AGATCT
BlpI 50 GCTGAGC GC^T[ACGT]AGC
1447 GCTGAGC
Bpu1102I 50 GCTGAGC GC^T[ACGT]AGC
1447 GCTGAGC
BsaAI 530 CACGTG [CT]AC^GT[GA]
1574 CACGTG
BsmI 2240 GAATGC GAATGC(1/-1)
2474 GAATGC
BssSI 335 CACGAG CACGAG(-5/-1)
1616 CACGAG
BstXI 1128 CCAACGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2852 CCAGATCCCTGG
CelII 50 GCTGAGC GC^T[ACGT]AGC
1447 GCTGAGC
DraIII 1268 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
2488 CACCAGGTG
Eam1105I 1139 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2144 GACAGCATGTC
Eco47III 2556 AGCGCT AGC^GCT
2968 AGCGCT
FspI 246 TGCGCA TGC^GCA
1026 TGCGCA
HincII 1862 GTCGAC GT[CT]^[GA]AC
2719 GTTAAC
HindII 1862 GTCGAC GT[CT]^[GA]AC
2719 GTTAAC
MaeI 23 CTAG C^TAG
467 CTAG
MseI 1385 TTAA T^TAA
2720 TTAA
NaeI 714 GCCGGC GCC^GGC
1342 GCCGGC
NgoMIV 714 GCCGGC G^CCGGC
1342 GCCGGC
PmaCI 530 CACGTG CAC^GTG
1574 CACGTG
PmlI 530 CACGTG CAC^GTG
1574 CACGTG
Tru9I 1385 TTAA T^TAA
2720 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvCI BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StuI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII