##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Aor51HI AscI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BglI BlnI BsaWI
BseAI BsiWI BsoBI BspDI BspEI
BspLU11I BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HpaI KasI KpnI MluI MroI
NaeI NarI NgoMIV NheI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmlI PshAI PvuI RsrII
SalI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
StuI SwaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 3467 GTCTAC GT^[AC][GT]AC
AclI 2153 AACGTT AA^CGTT
AcyI 167 GACGCC G[GA]^CG[CT]C
AflII 3153 CTTAAG C^TTAAG
Alw44I 1597 GTGCAC G^TGCAC
AlwI 1257 GATCC GGATC(4/5)
AocI 1551 CCTCAGG CC^T[ACGT]AGG
ApaI 1169 GGGCCC GGGCC^C
ApaLI 1597 GTGCAC G^TGCAC
AspI 141 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 861 GCTGAGG CCTCAGC(-5/-2)
BciVI 791 GGATAC GTATCC(6/5)
BfrI 3153 CTTAAG C^TTAAG
BlpI 1623 GCTAAGC GC^T[ACGT]AGC
BpmI 1073 CTGGAG CTGGAG(16/14)
Bpu1102I 1623 GCTAAGC GC^T[ACGT]AGC
BsaAI 1300 TACGTG [CT]AC^GT[GA]
BsaBI 3283 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 167 GACGCC G[GA]^CG[CT]C
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsmBI 1695 GAGACG CGTCTC(1/5)
BsrBI 340 GAGCGG CCGCTC(-3/-3)
BsrFI 391 ACCGGC [GA]^CCGG[CT]
Bsu36I 1551 CCTCAGG CC^T[ACGT]AGG
CelII 1623 GCTAAGC GC^T[ACGT]AGC
CfoI 3158 GCGC GCG^C
Cfr10I 391 ACCGGC [GA]^CCGG[CT]
DraIII 1297 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2463 GACAGCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
Eco81I 1551 CCTCAGG CC^T[ACGT]AGG
EcoNI 62 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 3158 GCGC GCG^C
Hin1I 167 GACGCC G[GA]^CG[CT]C
HinP1I 3158 GCGC G^CGC
HindIII 1887 AAGCTT A^AGCTT
KspI 8 CCGCGG CCGC^GG
MamI 3283 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1788 CAATTG C^AATTG
MunI 1788 CAATTG C^AATTG
NdeI 406 CATATG CA^TATG
NotI 4 GCGGCCGC GC^GGCCGC
PflFI 141 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 3371 GTTTAAAC GTTT^AAAC
Psp1406I 2153 AACGTT AA^CGTT
SacI 516 GAGCTC GAGCT^C
SacII 8 CCGCGG CCGC^GG
SapI 2445 GCTCTTC GCTCTTC(1/4)
SbfI 62 CCTGCAGG CCTGCA^GG
SexAI 977 ACCTGGT A^CC[AT]GGT
Sse8387I 62 CCTGCAGG CCTGCA^GG
SspI 3529 AATATT AAT^ATT
Tth111I 141 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 9 CGCG CG^CG
2138 CGCG
AflIII 2955 ACGTGT A^C[GA][CT]GT
3074 ACGTGT
AseI 3415 ATTAAT AT^TAAT
3497 ATTAAT
AsnI 3415 ATTAAT AT^TAAT
3497 ATTAAT
BalI 1201 TGGCCA TGG^CCA
3032 TGGCCA
BanI 359 GGTGCC G^G[CT][GA]CC
1014 GGCACC
BclI 3282 TGATCA T^GATCA
3288 TGATCA
BcnI 232 CCCGG CC^[GC]GG
871 CCGGG
BsmI 1749 GCATTC GAATGC(1/-1)
2606 GCATTC
BspHI 3227 TCATGA T^CATGA
3285 TCATGA
BssSI 1761 CACGAG CACGAG(-5/-1)
1594 CTCGTG
BstAPI 1296 GCACTACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2291 GCATCTCATGC
BstUI 9 CGCG CG^CG
2138 CGCG
EcoT14I 1198 CCATGG C^C[AT][AT]GG
2272 CCATGG
FbaI 3282 TGATCA T^GATCA
3288 TGATCA
HincII 164 GTTGAC GT[CT]^[GA]AC
1608 GTTGAC
HindII 164 GTTGAC GT[CT]^[GA]AC
1608 GTTGAC
MluNI 1201 TGGCCA TGG^CCA
3032 TGGCCA
MscI 1201 TGGCCA TGG^CCA
3032 TGGCCA
MvnI 9 CGCG CG^CG
2138 CGCG
NciI 232 CCCGG CC^[GC]GG
871 CCGGG
NcoI 1198 CCATGG C^CATGG
2272 CCATGG
NspI 1720 ACATGC [GA]CATG^[CT]
2617 GCATGT
PflMI 26 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1005 CCAGAGGTTGG
PshBI 3415 ATTAAT AT^TAAT
3497 ATTAAT
RcaI 3227 TCATGA T^CATGA
3285 TCATGA
StyI 1198 CCATGG C^C[AT][AT]GG
2272 CCATGG
Van91I 26 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1005 CCAGAGGTTGG
XcmI 823 CCAGTGTCATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1207 CCAGAAAGGAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI AvaII BanII BbsI
BbvI BfaI BglII BmyI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII