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# NO CUT ENZYMES ( 40 enzymes) #
##################################
AatII AccIII AgeI AhdI Aor51HI
AscI AspEI AviII AvrII BlnI
BsaWI BseAI BsiWI BspEI BssHII
Bst1107I BstZ17I CpoI Eam1105I Eco47III
EcoNI FseI FspI HpaI MluI
MroI NruI PacI PinAI PmeI
PshAI PvuI RsrII SfiI SgrAI
SmaI SnaBI SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 7567 GGTACC G^GTACC
AclI 4853 AACGTT AA^CGTT
ApaI 3643 GGGCCC GGGCC^C
Asp718I 7567 GGTACC G^GTACC
AspI 8137 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2565 TGGCCA TGG^CCA
BbrPI 8276 CACGTG CAC^GTG
BbvCI 4748 CCTCAGC CCTCAGC(-5/-2)
BciVI 6820 GGATAC GTATCC(6/5)
BclI 4105 TGATCA T^GATCA
BglI 7955 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 7361 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 7361 GCTGAGC GC^T[ACGT]AGC
BsmBI 6793 CGTCTC CGTCTC(1/5)
BspDI 4355 ATCGAT AT^CGAT
BsrFI 203 GCCGGC [GA]^CCGG[CT]
BssSI 1778 CACGAG CACGAG(-5/-1)
BstBI 5961 TTCGAA TT^CGAA
BstXI 3127 CCATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 7361 GCTGAGC GC^T[ACGT]AGC
Cfr10I 203 GCCGGC [GA]^CCGG[CT]
ClaI 4355 ATCGAT AT^CGAT
DraIII 993 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 883 ATGCAT ATGCA^T
FbaI 4105 TGATCA T^GATCA
KpnI 7567 GGTACC GGTAC^C
MluNI 2565 TGGCCA TGG^CCA
MscI 2565 TGGCCA TGG^CCA
NaeI 203 GCCGGC GCC^GGC
NdeI 1142 CATATG CA^TATG
NgoMIV 203 GCCGGC G^CCGGC
NheI 6980 GCTAGC G^CTAGC
NsiI 883 ATGCAT ATGCA^T
NspV 5961 TTCGAA TT^CGAA
PaeR7I 4581 CTCGAG C^TCGAG
PflFI 8137 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 8276 CACGTG CAC^GTG
PmlI 8276 CACGTG CAC^GTG
Psp1406I 4853 AACGTT AA^CGTT
SbfI 8207 CCTGCAGG CCTGCA^GG
ScaI 7028 AGTACT AGT^ACT
SfuI 5961 TTCGAA TT^CGAA
SphI 8081 GCATGC GCATG^C
Sse8387I 8207 CCTGCAGG CCTGCA^GG
Tth111I 8137 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 7001 TCTAGA T^CTAGA
XcmI 7854 CCAATACAAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4581 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BcgI 498 GCAAAAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4503 GCAAATCATTCG
BsaAI 2261 CACGTA [CT]AC^GT[GA]
8276 CACGTG
BsiEI 68 CGGCCG CG[GA][CT]^CG
172 CGGCCG
BspHI 8602 TCATGA T^CATGA
8623 TCATGA
DrdI 6380 GACTTATTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7034 GACGAGGATGTC
EagI 68 CGGCCG C^GGCCG
172 CGGCCG
EclXI 68 CGGCCG C^GGCCG
172 CGGCCG
Eco52I 68 CGGCCG C^GGCCG
172 CGGCCG
EcoRI 2209 GAATTC G^AATTC
6458 GAATTC
EcoRV 2590 GATATC GAT^ATC
4880 GATATC
MfeI 5030 CAATTG C^AATTG
8691 CAATTG
MunI 5030 CAATTG C^AATTG
8691 CAATTG
NotI 67 GCGGCCGC GC^GGCCGC
171 GCGGCCGC
RcaI 8602 TCATGA T^CATGA
8623 TCATGA
SacI 5204 GAGCTC GAGCT^C
8049 GAGCTC
SalI 3673 GTCGAC G^TCGAC
5544 GTCGAC
SapI 4712 GCTCTTC GCTCTTC(1/4)
5067 GCTCTTC
SpeI 4757 ACTAGT A^CTAGT
5323 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI Alw44I
AlwI AlwNI AocI ApaLI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BamHI BanI BanII BbeI
BbsI BbvI BcnI BfaI BfrI
BglII BmyI BpmI BpuAI BsaBI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshBI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfoI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI