##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccII AclI AflIII AgeI
Alw44I AlwI ApaI ApaLI AscI
AseI AsnI AspI AviII BamHI
BbeI BbrPI BcgI BclI BcnI
BglI BlpI Bpu1102I BsaAI BsiWI
Bsp1407I BspDI BspLU11I BsrGI BssHII
BssSI BstBI BstEII BstPI BstUI
CelII ClaI CpoI DraII DraIII
DrdI DsaI EagI EarI EclXI
Eco52I EcoO109I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HpaI KasI Ksp632I KspI MaeII
MfeI MluI MunI MvnI NarI
NciI NcoI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 2437 GGTACC G^GTACC
AccIII 670 TCCGGA T^CCGGA
AcyI 1071 GACGCC G[GA]^CG[CT]C
AhdI 508 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 635 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1407 AGCGCT AGC^GCT
Asp718I 2437 GGTACC G^GTACC
AspEI 508 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2128 CCTAGG C^CTAGG
BanII 641 GAGCTC G[GA]GC[CT]^C
BbsI 410 GAAGAC GAAGAC(2/6)
BbvCI 1360 CCTCAGC CCTCAGC(-5/-2)
BglII 706 AGATCT A^GATCT
BlnI 2128 CCTAGG C^CTAGG
BpuAI 410 GAAGAC GAAGAC(2/6)
BsaHI 1071 GACGCC G[GA]^CG[CT]C
BsaWI 670 TCCGGA [AT]^CCGG[AT]
BseAI 670 TCCGGA T^CCGGA
BsiEI 151 CGGTCG CG[GA][CT]^CG
BsmBI 1069 GAGACG CGTCTC(1/5)
BspEI 670 TCCGGA T^CCGGA
BspHI 2268 TCATGA T^CATGA
BsrBI 111 GAGCGG CCGCTC(-3/-3)
BsrFI 534 GCCGGC [GA]^CCGG[CT]
BstXI 751 CCATGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 706 AGATCT [GA]^GATC[CT]
Bsu36I 635 CCTGAGG CC^T[ACGT]AGG
CfoI 1408 GCGC GCG^C
Cfr10I 534 GCCGGC [GA]^CCGG[CT]
Eam1105I 508 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1407 AGCGCT AGC^GCT
Eco81I 635 CCTGAGG CC^T[ACGT]AGG
EcoNI 631 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 658 GCGGG CCCGC(4/6)
HaeII 1407 AGCGCT [GA]GCGC^[CT]
HgaI 1071 GACGC GACGC(5/10)
HhaI 1408 GCGC GCG^C
Hin1I 1071 GACGCC G[GA]^CG[CT]C
HinP1I 1408 GCGC G^CGC
HindIII 1099 AAGCTT A^AGCTT
KpnI 2437 GGTACC GGTAC^C
MflI 706 AGATCT [GA]^GATC[CT]
MroI 670 TCCGGA T^CCGGA
NaeI 534 GCCGGC GCC^GGC
NgoMIV 534 GCCGGC G^CCGGC
PstI 194 CTGCAG CTGCA^G
RcaI 2268 TCATGA T^CATGA
SacI 641 GAGCTC GAGCT^C
ScaI 1117 AGTACT AGT^ACT
SexAI 1785 ACCAGGT A^CC[AT]GGT
SspI 2958 AATATT AAT^ATT
XhoII 706 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 389 CTTAAG C^TTAAG
1541 CTTAAG
AlwNI 197 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
200 CAGCTGCTG
AvaI 241 CCCGAG C^[CT]CG[GA]G
519 CCCGAG
BalI 38 TGGCCA TGG^CCA
460 TGGCCA
BciVI 1389 GGATAC GTATCC(6/5)
2894 GGATAC
BfaI 1854 CTAG C^TAG
2129 CTAG
BfrI 389 CTTAAG C^TTAAG
1541 CTTAAG
BsgI 1501 GTGCAG GTGCAG(16/14)
1510 GTGCAG
BsmI 1331 GAATGC GAATGC(1/-1)
1447 GAATGC
BsoBI 241 CCCGAG C^[CT]CG[GA]G
519 CCCGAG
Bst1107I 903 GTATAC GTA^TAC
1982 GTATAC
BstZ17I 903 GTATAC GTA^TAC
1982 GTATAC
EaeI 38 TGGCCA [CT]^GGCC[GA]
460 TGGCCA
EcoRV 2264 GATATC GAT^ATC
2558 GATATC
EcoT14I 86 CCAAGG C^C[AT][AT]GG
2128 CCTAGG
MaeI 1854 CTAG C^TAG
2129 CTAG
MluNI 38 TGGCCA TGG^CCA
460 TGGCCA
MscI 38 TGGCCA TGG^CCA
460 TGGCCA
MspA1I 96 CAGCTG C[AC]G^C[GT]G
200 CAGCTG
NspI 81 GCATGC [GA]CATG^[CT]
2595 GCATGC
PflMI 916 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1786 CCAGGTGTTGG
PvuII 96 CAGCTG CAG^CTG
200 CAGCTG
SmlI 389 CTTAAG C^T[CT][GA]AG
1541 CTTAAG
SphI 81 GCATGC GCATG^C
2595 GCATGC
StuI 648 AGGCCT AGG^CCT
1182 AGGCCT
StyI 86 CCAAGG C^C[AT][AT]GG
2128 CCTAGG
TaqI 442 TCGA T^CGA
1294 TCGA
Tsp45I 842 GTGAC ^GT[GC]AC
2049 GTGAC
TthHB8I 442 TCGA T^CGA
1294 TCGA
Van91I 916 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1786 CCAGGTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
ApoI Asp700I AspHI AvaII BanI
BbvI BmyI BpmI BsaBI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MamI MboI MboII MnlI MseI
MslI MspI MvaI MwoI NdeI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp509I TspRI
XcmI XmnI