##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvrII BalI BamHI BbeI
BbrPI BbvCI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsiWI BsmI BspEI BsrBI
BssHII BssSI BstEII BstPI CelII
CpoI DraIII DrdI DsaI Eam1105I
Eco47III EcoNI EcoO65I EcoRV FbaI
FseI HaeII Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NarI
NcoI NdeI NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacI SacII SbfI SfiI SfoI
SgrAI SmlI SnaBI SpeI Sse8387I
StuI SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AccII 52 CGCG CG^CG
AclI 1655 AACGTT AA^CGTT
AocI 2112 CCTAAGG CC^T[ACGT]AGG
ApaI 65 GGGCCC GGGCC^C
AspHI 1355 GTGCTC G[AT]GC[AT]^C
AviII 302 TGCGCA TGC^GCA
BanII 65 GGGCCC G[GA]GC[CT]^C
BciVI 2818 GTATCC GTATCC(6/5)
BglI 601 GCCAGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1280 GAGACC GGTCTC(1/5)
BsaWI 836 ACCGGA [AT]^CCGG[AT]
BsgI 944 CTGCAC GTGCAG(16/14)
BsiEI 12 CGGCCG CG[GA][CT]^CG
BsiHKAI 1355 GTGCTC G[AT]GC[AT]^C
BsmBI 705 GAGACG CGTCTC(1/5)
Bsp1407I 3012 TGTACA T^GTACA
BspDI 1479 ATCGAT AT^CGAT
BspHI 1762 TCATGA T^CATGA
BsrFI 45 GCCGGC [GA]^CCGG[CT]
BsrGI 3012 TGTACA T^GTACA
Bst1107I 635 GTATAC GTA^TAC
BstAPI 513 GCATCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1489 TTCGAA TT^CGAA
BstUI 52 CGCG CG^CG
BstXI 1187 CCAAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 635 GTATAC GTA^TAC
Bsu36I 2112 CCTAAGG CC^T[ACGT]AGG
CfoI 303 GCGC GCG^C
Cfr10I 45 GCCGGC [GA]^CCGG[CT]
ClaI 1479 ATCGAT AT^CGAT
DraI 2545 TTTAAA TTT^AAA
EaeI 12 CGGCCG [CT]^GGCC[GA]
EagI 12 CGGCCG C^GGCCG
EclXI 12 CGGCCG C^GGCCG
Eco52I 12 CGGCCG C^GGCCG
Eco81I 2112 CCTAAGG CC^T[ACGT]AGG
FauI 771 GCGGG CCCGC(4/6)
FspI 302 TGCGCA TGC^GCA
HgaI 213 GCGTC GACGC(5/10)
HhaI 303 GCGC GCG^C
HinP1I 303 GCGC G^CGC
HincII 479 GTCGAC GT[CT]^[GA]AC
HindII 479 GTCGAC GT[CT]^[GA]AC
MvnI 52 CGCG CG^CG
NaeI 45 GCCGGC GCC^GGC
NgoMIV 45 GCCGGC G^CCGGC
NspV 1489 TTCGAA TT^CGAA
PflMI 2578 CCAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1655 AACGTT AA^CGTT
PstI 1872 CTGCAG CTGCA^G
PvuII 3016 CAGCTG CAG^CTG
RcaI 1762 TCATGA T^CATGA
SalI 479 GTCGAC G^TCGAC
SapI 2126 GAAGAGC GCTCTTC(1/4)
ScaI 1876 AGTACT AGT^ACT
SexAI 1829 ACCAGGT A^CC[AT]GGT
SfuI 1489 TTCGAA TT^CGAA
SmaI 62 CCCGGG CCC^GGG
SphI 1509 GCATGC GCATG^C
SspBI 3012 TGTACA T^GTACA
SspI 2374 AATATT AAT^ATT
Van91I 2578 CCAAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2800 TCTAGA T^CTAGA
XmaCI 62 CCCGGG C^CCGGG
XmaI 62 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 479 GTCGAC GT^[AC][GT]AC
635 GTATAC
AflIII 1777 ACATGT A^C[GA][CT]GT
2559 ACATGT
BcgI 2597 GCAGTTGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3032 GCAGCTAGTTCG
BglII 314 AGATCT A^GATCT
869 AGATCT
BspLU11I 1777 ACATGT A^CATGT
2559 ACATGT
BspMI 41 ACCTGC ACCTGC(4/8)
1942 ACCTGC
DraII 38 GGGACCT [GA]G^G[ACGT]CC[CT]
690 GGGTCCC
EcoO109I 38 GGGACCT [GA]G^G[ACGT]CC[CT]
690 GGGTCCC
EcoRI 1582 GAATTC G^AATTC
2328 GAATTC
EcoT14I 720 CCTTGG C^C[AT][AT]GG
1187 CCAAGG
EcoT22I 2259 ATGCAT ATGCA^T
2858 ATGCAT
MaeII 1656 ACGT A^CGT
2691 ACGT
MspA1I 2223 CAGCGG C[AC]G^C[GT]G
3016 CAGCTG
NsiI 2259 ATGCAT ATGCA^T
2858 ATGCAT
PpuMI 38 GGGACCT [GA]G^G[AT]CC[CT]
690 GGGTCCC
StyI 720 CCTTGG C^C[AT][AT]GG
1187 CCAAGG
Tsp45I 1685 GTCAC ^GT[GC]AC
2417 GTGAC
XcmI 1339 CCAAACTCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1743 CCAGACTCAACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AvaI AvaII
BanI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII