##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AviII BamHI BanI BbeI
BbrPI BfrI BglI BglII BsaAI
BsaBI BsaHI BseAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BsrBI
BssHII BstEII BstPI BstUI CfoI
ClaI CpoI DraIII Eam1105I Eco47III
EcoO65I EcoRI EcoRV FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MamI MluI MroI
MspA1I MvnI NarI NdeI NheI
NotI NruI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 1869 GTATAC GT^[AC][GT]AC
AclI 540 AACGTT AA^CGTT
Alw44I 268 GTGCAC G^TGCAC
AocI 1296 CCTGAGG CC^T[ACGT]AGG
ApaLI 268 GTGCAC G^TGCAC
BalI 3080 TGGCCA TGG^CCA
BanII 2849 GGGCTC G[GA]GC[CT]^C
BbvCI 1399 CCTCAGC CCTCAGC(-5/-2)
BcgI 989 GCATCCTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3047 TGATCA T^GATCA
BlpI 2732 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2732 GCTTAGC GC^T[ACGT]AGC
BsaI 54 GAGACC GGTCTC(1/5)
BsaWI 1337 ACCGGA [AT]^CCGG[AT]
BsgI 1638 GTGCAG GTGCAG(16/14)
BsiEI 23 CGGCCG CG[GA][CT]^CG
BspMI 882 GCAGGT ACCTGC(4/8)
Bst1107I 1869 GTATAC GTA^TAC
BstBI 1703 TTCGAA TT^CGAA
BstYI 629 GGATCT [GA]^GATC[CT]
BstZ17I 1869 GTATAC GTA^TAC
Bsu36I 1296 CCTGAGG CC^T[ACGT]AGG
CelII 2732 GCTTAGC GC^T[ACGT]AGC
DrdI 1749 GACACTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 23 CGGCCG C^GGCCG
EclXI 23 CGGCCG C^GGCCG
Eco52I 23 CGGCCG C^GGCCG
Eco81I 1296 CCTGAGG CC^T[ACGT]AGG
EcoT22I 2086 ATGCAT ATGCA^T
FauI 3041 GCGGG CCCGC(4/6)
FbaI 3047 TGATCA T^GATCA
FseI 1533 GGCCGGCC GGCCGG^CC
HincII 482 GTTGAC GT[CT]^[GA]AC
HindII 482 GTTGAC GT[CT]^[GA]AC
HindIII 2140 AAGCTT A^AGCTT
MfeI 688 CAATTG C^AATTG
MflI 629 GGATCT [GA]^GATC[CT]
MluNI 3080 TGGCCA TGG^CCA
MscI 3080 TGGCCA TGG^CCA
MslI 1211 CACCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 688 CAATTG C^AATTG
NsiI 2086 ATGCAT ATGCA^T
NspV 1703 TTCGAA TT^CGAA
PacI 720 TTAATTAA TTAAT^TAA
PaeR7I 87 CTCGAG C^TCGAG
Psp1406I 540 AACGTT AA^CGTT
PstI 1186 CTGCAG CTGCA^G
SfuI 1703 TTCGAA TT^CGAA
SphI 899 GCATGC GCATG^C
StuI 1794 AGGCCT AGG^CCT
SwaI 2950 ATTTAAAT ATTT^AAAT
XcmI 1807 CCACCCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 87 CTCGAG C^TCGAG
XhoII 629 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 883 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1174 CAGGGGCTG
AspHI 268 GTGCAC G[AT]GC[AT]^C
2681 GTGCTC
AvaI 87 CTCGAG C^[CT]CG[GA]G
3053 CCCGAG
AvrII 2133 CCTAGG C^CTAGG
3243 CCTAGG
BciVI 2151 GGATAC GTATCC(6/5)
2129 GTATCC
BlnI 2133 CCTAGG C^CTAGG
3243 CCTAGG
BsiHKAI 268 GTGCAC G[AT]GC[AT]^C
2681 GTGCTC
BsoBI 87 CTCGAG C^[CT]CG[GA]G
3053 CCCGAG
Bsp1407I 907 TGTACA T^GTACA
2792 TGTACA
BsrGI 907 TGTACA T^GTACA
2792 TGTACA
BstXI 1021 CCAGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3020 CCAGCACTTTGG
DsaI 837 CCATGG C^C[GA][CT]GG
950 CCATGG
EcoNI 172 CCTTTAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1611 CCTCTGGAAGG
HgaI 5 GACGC GACGC(5/10)
2036 GCGTC
NaeI 21 GCCGGC GCC^GGC
1534 GCCGGC
NcoI 837 CCATGG C^CATGG
950 CCATGG
NgoMIV 21 GCCGGC G^CCGGC
1534 GCCGGC
PflMI 1732 CCATCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1811 CCAGGAGGTGG
PpuMI 831 AGGACCC [GA]G^G[AT]CC[CT]
1410 GGGTCCC
SapI 920 GAAGAGC GCTCTTC(1/4)
1251 GAAGAGC
SexAI 934 ACCTGGT A^CC[AT]GGT
2835 ACCAGGT
SfcI 1186 CTGCAG C^T[GA][CT]AG
1238 CTGTAG
SmlI 87 CTCGAG C^T[CT][GA]AG
2305 CTCAAG
SspBI 907 TGTACA T^GTACA
2792 TGTACA
SspI 2372 AATATT AAT^ATT
2933 AATATT
Van91I 1732 CCATCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1811 CCAGGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AvaII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI Cac8I Cfr10I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI