##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvrII BamHI
BanI BbeI BbrPI BbsI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BspDI
BspEI BsrBI BsrFI BssHII Bst1107I
BstAPI BstBI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DrdI Eam1105I Eco47III Eco81I EcoRV
FbaI FseI HaeII Hin1I HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XcmI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 3063 ACATGT A^C[GA][CT]GT
AseI 3097 ATTAAT AT^TAAT
AsnI 3097 ATTAAT AT^TAAT
AvaII 464 GGACC G^G[AT]CC
AviII 69 TGCGCA TGC^GCA
BalI 283 TGGCCA TGG^CCA
BbvCI 2556 GCTGAGG CCTCAGC(-5/-2)
BciVI 3470 GTATCC GTATCC(6/5)
BsmBI 476 GAGACG CGTCTC(1/5)
BsmFI 2769 GGGAC GGGAC(10/14)
BspHI 729 TCATGA T^CATGA
BspLU11I 3063 ACATGT A^CATGT
BsrDI 1781 GCAATG GCAATG(2/0)
BssSI 719 CTCGTG CACGAG(-5/-1)
DraIII 2034 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2193 CCATGG C^C[GA][CT]GG
EcoNI 2680 CCTCGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 69 TGCGCA TGC^GCA
HgaI 94 GACGC GACGC(5/10)
MaeII 479 ACGT A^CGT
MluNI 283 TGGCCA TGG^CCA
MscI 283 TGGCCA TGG^CCA
NcoI 2193 CCATGG C^CATGG
PaeR7I 2681 CTCGAG C^TCGAG
PpuMI 463 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 3097 ATTAAT AT^TAAT
RcaI 729 TCATGA T^CATGA
SacI 235 GAGCTC GAGCT^C
SexAI 3394 ACCTGGT A^CC[AT]GGT
XhoI 2681 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 96 CGCG CG^CG
2582 CGCG
BanII 235 GAGCTC G[GA]GC[CT]^C
2787 GAGCCC
BcnI 229 CCCGG CC^[GC]GG
570 CCCGG
BseRI 433 GAGGAG GAGGAG(10/8)
2661 GAGGAG
BsgI 180 CTGCAC GTGCAG(16/14)
2488 GTGCAG
BsiEI 166 CGGCCG CG[GA][CT]^CG
240 CGGCCG
BstEII 525 GGTTACC G^GT[ACGT]ACC
3398 GGTAACC
BstPI 525 GGTTACC G^GT[ACGT]ACC
3398 GGTAACC
BstUI 96 CGCG CG^CG
2582 CGCG
DpnII 1396 GATC ^GATC
2892 GATC
EagI 166 CGGCCG C^GGCCG
240 CGGCCG
EclXI 166 CGGCCG C^GGCCG
240 CGGCCG
Eco52I 166 CGGCCG C^GGCCG
240 CGGCCG
EcoO65I 525 GGTTACC G^GT[ACGT]ACC
3398 GGTAACC
EcoRI 1919 GAATTC G^AATTC
3359 GAATTC
EcoT22I 2027 ATGCAT ATGCA^T
3010 ATGCAT
FauI 101 CCCGC CCCGC(4/6)
187 CCCGC
MboI 1396 GATC ^GATC
2892 GATC
MslI 643 CATCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3350 CATAATTGTG
MvnI 96 CGCG CG^CG
2582 CGCG
MwoI 2520 GCTGCAGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
3429 GCAGTGGTGGC
NciI 229 CCCGG CC^[GC]GG
570 CCCGG
NdeII 1396 GATC ^GATC
2892 GATC
NsiI 2027 ATGCAT ATGCA^T
3010 ATGCAT
PstI 851 CTGCAG CTGCA^G
2521 CTGCAG
SapI 270 GAAGAGC GCTCTTC(1/4)
2784 GAAGAGC
Sau3AI 1396 GATC ^GATC
2892 GATC
SphI 2029 GCATGC GCATG^C
2750 GCATGC
StuI 198 AGGCCT AGG^CCT
975 AGGCCT
TaqI 33 TCGA T^CGA
2682 TCGA
TfiI 799 GAATC G^A[AT]TC
1375 GAATC
TthHB8I 33 TCGA T^CGA
2682 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwNI ApoI Asp700I AspHI AvaI
BbvI BfaI BmyI BpmI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DraI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboII MnlI MseI
MspA1I MspI MvaI NlaIII NlaIV
NspI PleI PvuII RsaI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI XmnI