##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI AlwI Asp718I AspEI
AspI AvrII BamHI BbrPI BcgI
BciVI BfrI BglII BlnI BsaBI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrGI BstBI BstEII
BstPI BstYI ClaI DraIII DrdI
Eam1105I EcoO65I EcoRV EcoT22I FseI
HindIII KpnI MamI MflI MluI
MroI NdeI NheI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII SalI SapI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI SspBI SwaI
Tth111I XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 243 GACGTC GACGT^C
Alw44I 781 GTGCAC G^TGCAC
AlwNI 444 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 781 GTGCAC G^TGCAC
AscI 763 GGCGCGCC GG^CGCGCC
AseI 3132 ATTAAT AT^TAAT
AsnI 3132 ATTAAT AT^TAAT
AviII 218 TGCGCA TGC^GCA
BalI 1085 TGGCCA TGG^CCA
BbsI 3210 GTCTTC GAAGAC(2/6)
BclI 2413 TGATCA T^GATCA
BpuAI 3210 GTCTTC GAAGAC(2/6)
BsaWI 2028 ACCGGA [AT]^CCGG[AT]
BsmBI 1047 CGTCTC CGTCTC(1/5)
BspHI 2532 TCATGA T^CATGA
BsrDI 1667 GCAATG GCAATG(2/0)
Bst1107I 2692 GTATAC GTA^TAC
BstXI 845 CCACCCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2692 GTATAC GTA^TAC
CpoI 264 CGGACCG CG^G[AT]CCG
EcoNI 1410 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1733 GAATTC G^AATTC
FbaI 2413 TGATCA T^GATCA
FspI 218 TGCGCA TGC^GCA
HincII 2833 GTTAAC GT[CT]^[GA]AC
HindII 2833 GTTAAC GT[CT]^[GA]AC
HpaI 2833 GTTAAC GTT^AAC
MfeI 2887 CAATTG C^AATTG
MluNI 1085 TGGCCA TGG^CCA
MscI 1085 TGGCCA TGG^CCA
MunI 2887 CAATTG C^AATTG
PshBI 3132 ATTAAT AT^TAAT
RcaI 2532 TCATGA T^CATGA
RsrII 264 CGGACCG CG^G[AT]CCG
SacI 1948 GAGCTC GAGCT^C
SbfI 885 CCTGCAGG CCTGCA^GG
SfaNI 528 GCATC GCATC(5/9)
Sse8387I 885 CCTGCAGG CCTGCA^GG
StuI 953 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 832 GTCTAC GT^[AC][GT]AC
2692 GTATAC
AocI 949 CCTCAGG CC^T[ACGT]AGG
1886 CCTCAGG
Aor51HI 23 AGCGCT AGC^GCT
308 AGCGCT
ApaI 429 GGGCCC GGGCC^C
573 GGGCCC
Asp700I 306 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2434 GAATTTCTTC
BbvCI 460 CCTCAGC CCTCAGC(-5/-2)
1539 CCTCAGC
BlpI 678 GCTGAGC GC^T[ACGT]AGC
1031 GCTCAGC
Bpu1102I 678 GCTGAGC GC^T[ACGT]AGC
1031 GCTCAGC
BsaAI 835 TACGTG [CT]AC^GT[GA]
1291 TACGTG
BsaI 1336 GAGACC GGTCTC(1/5)
2538 GGTCTC
BssSI 2111 CTCGTG CACGAG(-5/-1)
2171 CTCGTG
BstAPI 1350 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1505 GCAACGCCTGC
Bsu36I 949 CCTCAGG CC^T[ACGT]AGG
1886 CCTCAGG
CelII 678 GCTGAGC GC^T[ACGT]AGC
1031 GCTCAGC
EarI 390 CTCTTC CTCTTC(1/4)
1830 CTCTTC
Eco47III 23 AGCGCT AGC^GCT
308 AGCGCT
Eco81I 949 CCTCAGG CC^T[ACGT]AGG
1886 CCTCAGG
Ksp632I 390 CTCTTC CTCTTC(1/4)
1830 CTCTTC
NaeI 1544 GCCGGC GCC^GGC
2001 GCCGGC
NgoMIV 1544 GCCGGC G^CCGGC
2001 GCCGGC
PflMI 1628 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2136 CCAGACAGTGG
SmlI 1951 CTCAAG C^T[CT][GA]AG
2491 CTTGAG
TfiI 2032 GATTC G^A[AT]TC
2167 GATTC
Van91I 1628 CCACCTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2136 CCAGACAGTGG
XmnI 306 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2434 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KspI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NotI PleI PpuMI PstI
RsaI SacII Sau3AI Sau96I ScrFI
SfcI SfoI SmaI SspI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XmaCI XmaI