##################################
# NO CUT ENZYMES ( 42 enzymes) #
##################################
AflII AgeI AhdI AscI AseI
AsnI Asp700I AspEI AvrII BfrI
BglII BlnI BsaBI BsmBI BssHII
Bst1107I BstBI BstZ17I Eam1105I EcoRV
EcoT22I HpaI MamI MfeI MluI
MunI NotI NsiI NspV PacI
PinAI PmeI PshBI SbfI SfiI
SfuI SgrAI SnaBI Sse8387I SspI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 3696 GGTACC G^GTACC
AclI 6159 AACGTT AA^CGTT
Asp718I 3696 GGTACC G^GTACC
AspI 3069 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 6985 CGAGGGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiWI 1185 CGTACG C^GTACG
BspHI 4628 TCATGA T^CATGA
DraI 6681 TTTAAA TTT^AAA
EcoRI 4392 GAATTC G^AATTC
FseI 2752 GGCCGGCC GGCCGG^CC
HindIII 5506 AAGCTT A^AGCTT
KpnI 3696 GGTACC GGTAC^C
NaeI 2753 GCCGGC GCC^GGC
NdeI 3842 CATATG CA^TATG
NgoMIV 2753 GCCGGC G^CCGGC
NheI 2613 GCTAGC G^CTAGC
NruI 6398 TCGCGA TCG^CGA
PflFI 3069 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 6085 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6159 AACGTT AA^CGTT
PvuI 4184 CGATCG CGAT^CG
RcaI 4628 TCATGA T^CATGA
SalI 5881 GTCGAC G^TCGAC
SapI 1264 GCTCTTC GCTCTTC(1/4)
SpeI 667 ACTAGT A^CTAGT
Tth111I 3069 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 4392 GAATTC [GA]^AATT[CT]
7649 AAATTT
ApoI 4392 GAATTC [GA]^AATT[CT]
7649 AAATTT
AviII 2417 TGCGCA TGC^GCA
4757 TGCGCA
BbeI 391 GGCGCC GGCGC^C
1548 GGCGCC
BciVI 1651 GGATAC GTATCC(6/5)
230 GTATCC
BclI 2768 TGATCA T^GATCA
4668 TGATCA
BsmI 4539 GAATGC GAATGC(1/-1)
6820 GCATTC
BspDI 4477 ATCGAT AT^CGAT
4519 ATCGAT
BspLU11I 5885 ACATGT A^CATGT
7191 ACATGT
ClaI 4477 ATCGAT AT^CGAT
4519 ATCGAT
CpoI 166 CGGACCG CG^G[AT]CCG
2180 CGGTCCG
DraIII 1984 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3553 CACCTGGTG
DrdI 441 GACTGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3241 GACATCAAGGTC
EcoNI 2931 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4707 CCTTATTCAGG
FbaI 2768 TGATCA T^GATCA
4668 TGATCA
FspI 2417 TGCGCA TGC^GCA
4757 TGCGCA
HincII 5584 GTTGAC GT[CT]^[GA]AC
5881 GTCGAC
HindII 5584 GTTGAC GT[CT]^[GA]AC
5881 GTCGAC
KasI 391 GGCGCC G^GCGCC
1548 GGCGCC
NarI 391 GGCGCC GG^CGCC
1548 GGCGCC
PaeR7I 4457 CTCGAG C^TCGAG
5209 CTCGAG
RsrII 166 CGGACCG CG^G[AT]CCG
2180 CGGTCCG
SfoI 391 GGCGCC GGC^GCC
1548 GGCGCC
StuI 4889 AGGCCT AGG^CCT
5408 AGGCCT
XbaI 3774 TCTAGA T^CTAGA
7405 TCTAGA
XhoI 4457 CTCGAG C^TCGAG
5209 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 164 enzymes) #
#########################################
AatII AccI AccII AccIII AciI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AspHI AvaI AvaII BalI
BamHI BanI BanII BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScaI ScrFI SexAI SfaNI
SfcI SmaI SmlI SphI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI