##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
Acc65I AccIII AclI AflII AhdI
AscI Asp718I AspEI BbrPI BciVI
BfrI BsaBI BseAI BspEI Bst1107I
BstBI BstZ17I CpoI DraI Eam1105I
EcoRV HpaI KpnI MamI MfeI
MluI MroI MunI NdeI NheI
NruI NspV PacI PmaCI PmeI
PmlI Psp1406I RsrII SfuI SnaBI
SpeI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 8581 GAATTC [GA]^AATT[CT]
AgeI 770 ACCGGT A^CCGGT
ApoI 8581 GAATTC [GA]^AATT[CT]
AseI 8388 ATTAAT AT^TAAT
AsnI 8388 ATTAAT AT^TAAT
AspI 2264 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 8611 TGCGCA TGC^GCA
AvrII 20 CCTAGG C^CTAGG
BlnI 20 CCTAGG C^CTAGG
BsaAI 4443 TACGTG [CT]AC^GT[GA]
BsiWI 4772 CGTACG C^GTACG
BspDI 1458 ATCGAT AT^CGAT
BspLU11I 1621 ACATGT A^CATGT
BsrDI 4919 CATTGC GCAATG(2/0)
ClaI 1458 ATCGAT AT^CGAT
DrdI 1605 GACCGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 8581 GAATTC G^AATTC
EcoT22I 8055 ATGCAT ATGCA^T
FseI 2950 GGCCGGCC GGCCGG^CC
FspI 8611 TGCGCA TGC^GCA
NsiI 8055 ATGCAT ATGCA^T
PaeR7I 3708 CTCGAG C^TCGAG
PflFI 2264 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 770 ACCGGT A^CCGGT
PshAI 6989 GACGTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 8388 ATTAAT AT^TAAT
PvuI 86 CGATCG CGAT^CG
SalI 5114 GTCGAC G^TCGAC
ScaI 1324 AGTACT AGT^ACT
Tth111I 2264 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3708 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 5114 GTCGAC GT^[AC][GT]AC
6138 GTCTAC
AflIII 1621 ACATGT A^C[GA][CT]GT
8641 ACACGT
AocI 1990 CCTCAGG CC^T[ACGT]AGG
6047 CCTGAGG
Aor51HI 1231 AGCGCT AGC^GCT
8125 AGCGCT
BamHI 339 GGATCC G^GATCC
4429 GGATCC
BclI 3541 TGATCA T^GATCA
7531 TGATCA
BlpI 6818 GCTCAGC GC^T[ACGT]AGC
8121 GCTTAGC
Bpu1102I 6818 GCTCAGC GC^T[ACGT]AGC
8121 GCTTAGC
BsmBI 1781 GAGACG CGTCTC(1/5)
4263 GAGACG
BspHI 1130 TCATGA T^CATGA
1615 TCATGA
Bsu36I 1990 CCTCAGG CC^T[ACGT]AGG
6047 CCTGAGG
CelII 6818 GCTCAGC GC^T[ACGT]AGC
8121 GCTTAGC
DraIII 1440 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
6892 CACCCAGTG
Eco47III 1231 AGCGCT AGC^GCT
8125 AGCGCT
Eco81I 1990 CCTCAGG CC^T[ACGT]AGG
6047 CCTGAGG
FbaI 3541 TGATCA T^GATCA
7531 TGATCA
HincII 2556 GTCAAC GT[CT]^[GA]AC
5114 GTCGAC
HindII 2556 GTCAAC GT[CT]^[GA]AC
5114 GTCGAC
HindIII 287 AAGCTT A^AGCTT
6639 AAGCTT
NotI 4685 GCGGCCGC GC^GGCCGC
6674 GCGGCCGC
RcaI 1130 TCATGA T^CATGA
1615 TCATGA
SapI 4880 GAAGAGC GCTCTTC(1/4)
6579 GAAGAGC
SbfI 5060 CCTGCAGG CCTGCA^GG
7747 CCTGCAGG
Sse8387I 5060 CCTGCAGG CCTGCA^GG
7747 CCTGCAGG
TfiI 1061 GATTC G^A[AT]TC
1193 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 158 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SfiI SfoI SgrAI SmaI
SmlI SphI SspBI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI