##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AcyI AgeI Alw44I
AocI Aor51HI ApaLI AscI AvaI
AviII AvrII BamHI BbeI BbrPI
BcgI BlnI BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BsrBI BssHII
BstEII BstPI Bsu36I ClaI CpoI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I FseI FspI Hin1I HpaI
KasI KspI MluI MroI NaeI
NarI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 3270 GGTACC G^GTACC
AclI 1545 AACGTT AA^CGTT
AflIII 3312 ACATGT A^C[GA][CT]GT
AhdI 6118 GACCGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 8638 GGGCCC GGGCC^C
Asp718I 3270 GGTACC G^GTACC
AspEI 6118 GACCGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 5698 TGGCCA TGG^CCA
BclI 8039 TGATCA T^GATCA
BglI 5016 GCCAGACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 7662 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 7662 GCTAAGC GC^T[ACGT]AGC
BsaI 3632 GAGACC GGTCTC(1/5)
Bsp1407I 7785 TGTACA T^GTACA
BspLU11I 3312 ACATGT A^CATGT
BsrFI 5021 ACCGGC [GA]^CCGG[CT]
BsrGI 7785 TGTACA T^GTACA
Bst1107I 6720 GTATAC GTA^TAC
BstAPI 4614 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 6720 GTATAC GTA^TAC
CelII 7662 GCTAAGC GC^T[ACGT]AGC
Cfr10I 5021 ACCGGC [GA]^CCGG[CT]
DraIII 6966 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4662 GACCGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 6118 GACCGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 4221 GCGGG CCCGC(4/6)
FbaI 8039 TGATCA T^GATCA
HgaI 5054 GACGC GACGC(5/10)
HincII 8268 GTTGAC GT[CT]^[GA]AC
HindII 8268 GTTGAC GT[CT]^[GA]AC
KpnI 3270 GGTACC GGTAC^C
MfeI 4465 CAATTG C^AATTG
MluNI 5698 TGGCCA TGG^CCA
MscI 5698 TGGCCA TGG^CCA
MunI 4465 CAATTG C^AATTG
NdeI 1880 CATATG CA^TATG
PshAI 3634 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1545 AACGTT AA^CGTT
SphI 6936 GCATGC GCATG^C
SspBI 7785 TGTACA T^GTACA
XbaI 5483 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 5050 CGCG CG^CG
5056 CGCG
BseRI 4548 CTCCTC GAGGAG(10/8)
2128 GAGGAG
BsrDI 1737 CATTGC GCAATG(2/0)
8336 CATTGC
BssSI 2389 CACGAG CACGAG(-5/-1)
7015 CACGAG
BstBI 1404 TTCGAA TT^CGAA
2703 TTCGAA
BstUI 5050 CGCG CG^CG
5056 CGCG
EaeI 5517 CGGCCA [CT]^GGCC[GA]
5698 TGGCCA
EcoNI 5192 CCTGGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
8155 CCTGGGCTAGG
EcoRI 2382 GAATTC G^AATTC
2706 GAATTC
EcoT22I 1923 ATGCAT ATGCA^T
2611 ATGCAT
HaeII 1068 AGCGCC [GA]GCGC^[CT]
2065 AGCGCC
MvnI 5050 CGCG CG^CG
5056 CGCG
NsiI 1923 ATGCAT ATGCA^T
2611 ATGCAT
NspI 3312 ACATGT [GA]CATG^[CT]
6936 GCATGC
NspV 1404 TTCGAA TT^CGAA
2703 TTCGAA
SacI 3845 GAGCTC GAGCT^C
6199 GAGCTC
SapI 1901 GCTCTTC GCTCTTC(1/4)
3104 GCTCTTC
ScaI 757 AGTACT AGT^ACT
4713 AGTACT
SfuI 1404 TTCGAA TT^CGAA
2703 TTCGAA
XcmI 1143 CCATACTACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3584 CCAAAGCCAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AspI AvaII
BanI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BfrI BglII
BmyI BpmI BpuAI BsaBI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoRV EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PflFI
PflMI PleI PpuMI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII XmnI