################################## # NO CUT ENZYMES ( 32 enzymes) # ################################## AclI AgeI AscI BsiWI BspDI BssSI BstBI ClaI CpoI DraIII EcoRV FseI MluI NaeI NgoMIV NheI NruI NspV PacI PinAI PshAI Psp1406I PvuI RsrII SalI SbfI SfiI SfuI SgrAI SnaBI Sse8387I XbaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 1777 GACGTC GACGT^C AccIII 705 TCCGGA T^CCGGA AflII 2158 CTTAAG C^TTAAG AhdI 5334 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 280 CCTCAGG CC^T[ACGT]AGG ApaI 4803 GGGCCC GGGCC^C AspEI 5334 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 4686 CCTAGG C^CTAGG BalI 4265 TGGCCA TGG^CCA BbeI 827 GGCGCC GGCGC^C BfrI 2158 CTTAAG C^TTAAG BglII 6254 AGATCT A^GATCT BlnI 4686 CCTAGG C^CTAGG BsaWI 705 TCCGGA [AT]^CCGG[AT] BseAI 705 TCCGGA T^CCGGA BspEI 705 TCCGGA T^CCGGA BsrBI 759 CCGCTC CCGCTC(-3/-3) BsrFI 4903 ACCGGC [GA]^CCGG[CT] Bst1107I 7116 GTATAC GTA^TAC BstEII 4681 GGTCACC G^GT[ACGT]ACC BstPI 4681 GGTCACC G^GT[ACGT]ACC BstZ17I 7116 GTATAC GTA^TAC Bsu36I 280 CCTCAGG CC^T[ACGT]AGG Cfr10I 4903 ACCGGC [GA]^CCGG[CT] DrdI 4319 GACTCCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1553 CGGCCG C^GGCCG Eam1105I 5334 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1553 CGGCCG C^GGCCG Eco52I 1553 CGGCCG C^GGCCG Eco81I 280 CCTCAGG CC^T[ACGT]AGG EcoNI 6088 CCTATTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 4681 GGTCACC G^GT[ACGT]ACC KasI 827 GGCGCC G^GCGCC MluNI 4265 TGGCCA TGG^CCA MroI 705 TCCGGA T^CCGGA MscI 4265 TGGCCA TGG^CCA NarI 827 GGCGCC GG^CGCC NcoI 5774 CCATGG C^CATGG NdeI 3134 CATATG CA^TATG NotI 1552 GCGGCCGC GC^GGCCGC PaeR7I 4004 CTCGAG C^TCGAG PmeI 6934 GTTTAAAC GTTT^AAAC SapI 6280 GCTCTTC GCTCTTC(1/4) SexAI 6532 ACCAGGT A^CC[AT]GGT SfoI 827 GGCGCC GGC^GCC SphI 4097 GCATGC GCATG^C StuI 3204 AGGCCT AGG^CCT XhoI 4004 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 827 GGCGCC G[GA]^CG[CT]C 1777 GACGTC AseI 1071 ATTAAT AT^TAAT 7006 ATTAAT AsnI 1071 ATTAAT AT^TAAT 7006 ATTAAT AviII 270 TGCGCA TGC^GCA 1394 TGCGCA BamHI 531 GGATCC G^GATCC 3415 GGATCC BclI 1985 TGATCA T^GATCA 7095 TGATCA BsaHI 827 GGCGCC G[GA]^CG[CT]C 1777 GACGTC Bsp1407I 4181 TGTACA T^GTACA 6480 TGTACA BspHI 3969 TCATGA T^CATGA 7399 TCATGA BsrGI 4181 TGTACA T^GTACA 6480 TGTACA BstXI 3896 CCAATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5368 CCAGTTTTGTGG EcoT22I 3309 ATGCAT ATGCA^T 7300 ATGCAT FbaI 1985 TGATCA T^GATCA 7095 TGATCA FspI 270 TGCGCA TGC^GCA 1394 TGCGCA HgaI 4917 GACGC GACGC(5/10) 2850 GCGTC Hin1I 827 GGCGCC G[GA]^CG[CT]C 1777 GACGTC HpaI 5920 GTTAAC GTT^AAC 7064 GTTAAC KspI 614 CCGCGG CCGC^GG 1416 CCGCGG MfeI 6346 CAATTG C^AATTG 6701 CAATTG MunI 6346 CAATTG C^AATTG 6701 CAATTG NsiI 3309 ATGCAT ATGCA^T 7300 ATGCAT PshBI 1071 ATTAAT AT^TAAT 7006 ATTAAT RcaI 3969 TCATGA T^CATGA 7399 TCATGA SacII 614 CCGCGG CCGC^GG 1416 CCGCGG SmaI 860 CCCGGG CCC^GGG 8343 CCCGGG SpeI 3951 ACTAGT A^CTAGT 5235 ACTAGT SspBI 4181 TGTACA T^GTACA 6480 TGTACA SwaI 3991 ATTTAAAT ATTT^AAAT 7618 ATTTAAAT XcmI 1704 CCAGGTGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3133 CCATATGAATAGTGG XmaCI 860 CCCGGG C^CCGGG 8343 CCCGGG XmaI 860 CCCGGG C^CCGGG 8343 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 147 enzymes) # ######################################### Acc65I AccI AccII AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI Aor51HI ApaLI ApoI Asp700I Asp718I AspHI AspI AvaI AvaII BanI BanII BbrPI BbsI BbvCI BbvI BcgI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrDI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco47III EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XhoII XmnI