##################################
# NO CUT ENZYMES ( 32 enzymes) #
##################################
AclI AgeI AscI BsiWI BspDI
BssSI BstBI ClaI CpoI DraIII
EcoRV FseI MluI NaeI NgoMIV
NheI NruI NspV PacI PinAI
PshAI Psp1406I PvuI RsrII SalI
SbfI SfiI SfuI SgrAI SnaBI
Sse8387I XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 1777 GACGTC GACGT^C
AccIII 705 TCCGGA T^CCGGA
AflII 2158 CTTAAG C^TTAAG
AhdI 5334 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 280 CCTCAGG CC^T[ACGT]AGG
ApaI 4803 GGGCCC GGGCC^C
AspEI 5334 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4686 CCTAGG C^CTAGG
BalI 4265 TGGCCA TGG^CCA
BbeI 827 GGCGCC GGCGC^C
BfrI 2158 CTTAAG C^TTAAG
BglII 6254 AGATCT A^GATCT
BlnI 4686 CCTAGG C^CTAGG
BsaWI 705 TCCGGA [AT]^CCGG[AT]
BseAI 705 TCCGGA T^CCGGA
BspEI 705 TCCGGA T^CCGGA
BsrBI 759 CCGCTC CCGCTC(-3/-3)
BsrFI 4903 ACCGGC [GA]^CCGG[CT]
Bst1107I 7116 GTATAC GTA^TAC
BstEII 4681 GGTCACC G^GT[ACGT]ACC
BstPI 4681 GGTCACC G^GT[ACGT]ACC
BstZ17I 7116 GTATAC GTA^TAC
Bsu36I 280 CCTCAGG CC^T[ACGT]AGG
Cfr10I 4903 ACCGGC [GA]^CCGG[CT]
DrdI 4319 GACTCCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1553 CGGCCG C^GGCCG
Eam1105I 5334 GACCAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1553 CGGCCG C^GGCCG
Eco52I 1553 CGGCCG C^GGCCG
Eco81I 280 CCTCAGG CC^T[ACGT]AGG
EcoNI 6088 CCTATTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4681 GGTCACC G^GT[ACGT]ACC
KasI 827 GGCGCC G^GCGCC
MluNI 4265 TGGCCA TGG^CCA
MroI 705 TCCGGA T^CCGGA
MscI 4265 TGGCCA TGG^CCA
NarI 827 GGCGCC GG^CGCC
NcoI 5774 CCATGG C^CATGG
NdeI 3134 CATATG CA^TATG
NotI 1552 GCGGCCGC GC^GGCCGC
PaeR7I 4004 CTCGAG C^TCGAG
PmeI 6934 GTTTAAAC GTTT^AAAC
SapI 6280 GCTCTTC GCTCTTC(1/4)
SexAI 6532 ACCAGGT A^CC[AT]GGT
SfoI 827 GGCGCC GGC^GCC
SphI 4097 GCATGC GCATG^C
StuI 3204 AGGCCT AGG^CCT
XhoI 4004 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 827 GGCGCC G[GA]^CG[CT]C
1777 GACGTC
AseI 1071 ATTAAT AT^TAAT
7006 ATTAAT
AsnI 1071 ATTAAT AT^TAAT
7006 ATTAAT
AviII 270 TGCGCA TGC^GCA
1394 TGCGCA
BamHI 531 GGATCC G^GATCC
3415 GGATCC
BclI 1985 TGATCA T^GATCA
7095 TGATCA
BsaHI 827 GGCGCC G[GA]^CG[CT]C
1777 GACGTC
Bsp1407I 4181 TGTACA T^GTACA
6480 TGTACA
BspHI 3969 TCATGA T^CATGA
7399 TCATGA
BsrGI 4181 TGTACA T^GTACA
6480 TGTACA
BstXI 3896 CCAATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5368 CCAGTTTTGTGG
EcoT22I 3309 ATGCAT ATGCA^T
7300 ATGCAT
FbaI 1985 TGATCA T^GATCA
7095 TGATCA
FspI 270 TGCGCA TGC^GCA
1394 TGCGCA
HgaI 4917 GACGC GACGC(5/10)
2850 GCGTC
Hin1I 827 GGCGCC G[GA]^CG[CT]C
1777 GACGTC
HpaI 5920 GTTAAC GTT^AAC
7064 GTTAAC
KspI 614 CCGCGG CCGC^GG
1416 CCGCGG
MfeI 6346 CAATTG C^AATTG
6701 CAATTG
MunI 6346 CAATTG C^AATTG
6701 CAATTG
NsiI 3309 ATGCAT ATGCA^T
7300 ATGCAT
PshBI 1071 ATTAAT AT^TAAT
7006 ATTAAT
RcaI 3969 TCATGA T^CATGA
7399 TCATGA
SacII 614 CCGCGG CCGC^GG
1416 CCGCGG
SmaI 860 CCCGGG CCC^GGG
8343 CCCGGG
SpeI 3951 ACTAGT A^CTAGT
5235 ACTAGT
SspBI 4181 TGTACA T^GTACA
6480 TGTACA
SwaI 3991 ATTTAAAT ATTT^AAAT
7618 ATTTAAAT
XcmI 1704 CCAGGTGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3133 CCATATGAATAGTGG
XmaCI 860 CCCGGG C^CCGGG
8343 CCCGGG
XmaI 860 CCCGGG C^CCGGG
8343 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AfaI AflIII AluI Alw44I AlwI
AlwNI Aor51HI ApaLI ApoI Asp700I
Asp718I AspHI AspI AvaI AvaII
BanI BanII BbrPI BbsI BbvCI
BbvI BcgI BciVI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco47III
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII XmnI