##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccI AccII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AviII AvrII BamHI
BbrPI BbvCI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BsiWI
BsmBI BspDI BspLU11I BssHII Bst1107I
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI KspI MfeI MflI
MluI MunI MvnI NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 2218 GGTACC G^GTACC
AcyI 39 GGCGCC G[GA]^CG[CT]C
AlwI 2167 GATCC GGATC(4/5)
Aor51HI 234 AGCGCT AGC^GCT
Asp718I 2218 GGTACC G^GTACC
AspI 1697 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1016 CTCGGG C^[CT]CG[GA]G
BbeI 39 GGCGCC GGCGC^C
BsaAI 730 TACGTG [CT]AC^GT[GA]
BsaBI 2161 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 39 GGCGCC G[GA]^CG[CT]C
BsaI 1702 GGTCTC GGTCTC(1/5)
BsiEI 2364 CGACCG CG[GA][CT]^CG
BsoBI 1016 CTCGGG C^[CT]CG[GA]G
Bsp1407I 984 TGTACA T^GTACA
BsrGI 984 TGTACA T^GTACA
BssSI 190 CTCGTG CACGAG(-5/-1)
BstBI 2432 TTCGAA TT^CGAA
Eco47III 234 AGCGCT AGC^GCT
EcoNI 1713 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 39 GGCGCC G[GA]^CG[CT]C
KasI 39 GGCGCC G^GCGCC
KpnI 2218 GGTACC GGTAC^C
MamI 2161 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1334 TTAA T^TAA
NaeI 911 GCCGGC GCC^GGC
NarI 39 GGCGCC GG^CGCC
NcoI 2198 CCATGG C^CATGG
NgoMIV 911 GCCGGC G^CCGGC
NspI 659 ACATGC [GA]CATG^[CT]
NspV 2432 TTCGAA TT^CGAA
PflFI 1697 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1345 GCTCTTC GCTCTTC(1/4)
SfoI 39 GGCGCC GGC^GCC
SfuI 2432 TTCGAA TT^CGAA
SgrAI 41 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 984 TGTACA T^GTACA
Tru9I 1334 TTAA T^TAA
Tth111I 1697 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 1379 TCCGGA T^CCGGA
2072 TCCGGA
AcsI 304 AAATTT [GA]^AATT[CT]
2149 AAATTC
ApoI 304 AAATTT [GA]^AATT[CT]
2149 AAATTC
BsaWI 1379 TCCGGA [AT]^CCGG[AT]
2072 TCCGGA
BseAI 1379 TCCGGA T^CCGGA
2072 TCCGGA
BspEI 1379 TCCGGA T^CCGGA
2072 TCCGGA
BspHI 480 TCATGA T^CATGA
2157 TCATGA
BsrBI 99 GAGCGG CCGCTC(-3/-3)
109 GAGCGG
BstAPI 1842 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2782 GCAGGCCATGC
BstXI 1787 CCAACACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2198 CCATGGGCATGG
EcoT22I 686 ATGCAT ATGCA^T
2789 ATGCAT
HaeII 39 GGCGCC [GA]GCGC^[CT]
234 AGCGCT
MaeII 731 ACGT A^CGT
2509 ACGT
MroI 1379 TCCGGA T^CCGGA
2072 TCCGGA
NdeI 689 CATATG CA^TATG
1811 CATATG
NsiI 686 ATGCAT ATGCA^T
2789 ATGCAT
PflMI 316 CCACACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2556 CCAGGCAGTGG
PstI 2764 CTGCAG CTGCA^G
2818 CTGCAG
RcaI 480 TCATGA T^CATGA
2157 TCATGA
SacI 1343 GAGCTC GAGCT^C
1660 GAGCTC
SfcI 2764 CTGCAG C^T[GA][CT]AG
2818 CTGCAG
SmlI 433 CTCAAG C^T[CT][GA]AG
1663 CTCAAG
Van91I 316 CCACACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2556 CCAGGCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BalI BanI BanII BbsI
BbvI BciVI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI