##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BcgI
BglI BglII BlnI BpuAI BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoT22I FauI FseI
FspI HaeII HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1973 GTATAC GT^[AC][GT]AC
AclI 2214 AACGTT AA^CGTT
AcyI 2524 GGCGTC G[GA]^CG[CT]C
AflII 1889 CTTAAG C^TTAAG
AocI 409 CCTAAGG CC^T[ACGT]AGG
AseI 2272 ATTAAT AT^TAAT
AsnI 2272 ATTAAT AT^TAAT
BbvCI 2566 CCTCAGC CCTCAGC(-5/-2)
BciVI 1121 GTATCC GTATCC(6/5)
BclI 398 TGATCA T^GATCA
BfrI 1889 CTTAAG C^TTAAG
BlpI 1414 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1414 GCTCAGC GC^T[ACGT]AGC
BsaBI 2464 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2524 GGCGTC G[GA]^CG[CT]C
BsgI 430 GTGCAG GTGCAG(16/14)
BspMI 591 GCAGGT ACCTGC(4/8)
BsrDI 1037 GCAATG GCAATG(2/0)
Bst1107I 1973 GTATAC GTA^TAC
BstXI 2313 CCAGTATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1973 GTATAC GTA^TAC
Bsu36I 409 CCTAAGG CC^T[ACGT]AGG
CelII 1414 GCTCAGC GC^T[ACGT]AGC
CfoI 1146 GCGC GCG^C
DraI 1995 TTTAAA TTT^AAA
DsaI 725 CCATGG C^C[GA][CT]GG
Eco81I 409 CCTAAGG CC^T[ACGT]AGG
EcoRV 1511 GATATC GAT^ATC
FbaI 398 TGATCA T^GATCA
HgaI 2525 GCGTC GACGC(5/10)
HhaI 1146 GCGC GCG^C
Hin1I 2524 GGCGTC G[GA]^CG[CT]C
HinP1I 1146 GCGC G^CGC
HincII 326 GTCAAC GT[CT]^[GA]AC
HindII 326 GTCAAC GT[CT]^[GA]AC
MamI 2464 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 487 CAGCTG C[AC]G^C[GT]G
NcoI 725 CCATGG C^CATGG
NdeI 1377 CATATG CA^TATG
PaeR7I 293 CTCGAG C^TCGAG
PshBI 2272 ATTAAT AT^TAAT
Psp1406I 2214 AACGTT AA^CGTT
PstI 1766 CTGCAG CTGCA^G
PvuII 487 CAGCTG CAG^CTG
SmaI 425 CCCGGG CCC^GGG
SspI 2509 AATATT AAT^ATT
XbaI 931 TCTAGA T^CTAGA
XcmI 2582 CCATATATATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 293 CTCGAG C^TCGAG
XmaCI 425 CCCGGG C^CCGGG
XmaI 425 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Asp700I 1386 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2016 GAACATTTTC
AspHI 319 GAGCTC G[AT]GC[AT]^C
415 GAGCTC
BsaI 280 GAGACC GGTCTC(1/5)
346 GAGACC
BsiHKAI 319 GAGCTC G[AT]GC[AT]^C
415 GAGCTC
BsmI 1065 GAATGC GAATGC(1/-1)
1756 GCATTC
BstYI 365 AGATCC [GA]^GATC[CT]
492 GGATCT
Cac8I 1069 GCAGGC GC[ACGT]^[ACGT]GC
1884 GCAGGC
EarI 277 GAAGAG CTCTTC(1/4)
692 GAAGAG
HapII 315 CCGG C^CGG
426 CCGG
HindIII 664 AAGCTT A^AGCTT
1304 AAGCTT
HpaII 315 CCGG C^CGG
426 CCGG
Ksp632I 277 GAAGAG CTCTTC(1/4)
692 GAAGAG
MflI 365 AGATCC [GA]^GATC[CT]
492 GGATCT
MspI 315 CCGG C^CGG
426 CCGG
PflMI 123 CCATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
825 CCAGCACTTGG
PpuMI 9 GGGTCCT [GA]G^G[AT]CC[CT]
288 AGGACCT
SacI 319 GAGCTC GAGCT^C
415 GAGCTC
SmlI 293 CTCGAG C^T[CT][GA]AG
1889 CTTAAG
Van91I 123 CCATTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
825 CCAGCACTTGG
XhoII 365 AGATCC [GA]^GATC[CT]
492 GGATCT
XmnI 1386 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2016 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsoBI
Bsp1286I BspHI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DraII
EcoNI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I