##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AgeI AhdI Aor51HI AscI Asp718I
AspEI AviII BsiEI BsiWI BspHI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstUI BstZ17I Cfr10I CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FseI FspI KpnI
KspI MluI MvnI NaeI NgoMIV
NotI NruI NsiI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SgrAI SnaBI SpeI Sse8387I
StuI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 5372 TCCGGA T^CCGGA
ApaI 3091 GGGCCC GGGCC^C
BbeI 80 GGCGCC GGCGC^C
BcgI 4410 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4694 GGATAC GTATCC(6/5)
BclI 1742 TGATCA T^GATCA
BglI 591 GCCCGTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 354 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 354 GCTAAGC GC^T[ACGT]AGC
BsaBI 5501 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 5372 TCCGGA [AT]^CCGG[AT]
BseAI 5372 TCCGGA T^CCGGA
BspDI 4158 ATCGAT AT^CGAT
BspEI 5372 TCCGGA T^CCGGA
BssSI 2626 CTCGTG CACGAG(-5/-1)
BstBI 493 TTCGAA TT^CGAA
BstEII 2882 GGTGACC G^GT[ACGT]ACC
BstPI 2882 GGTGACC G^GT[ACGT]ACC
CelII 354 GCTAAGC GC^T[ACGT]AGC
ClaI 4158 ATCGAT AT^CGAT
DrdI 6955 GACCTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2882 GGTGACC G^GT[ACGT]ACC
FbaI 1742 TGATCA T^GATCA
HgaI 3925 GACGC GACGC(5/10)
KasI 80 GGCGCC G^GCGCC
MamI 5501 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 5372 TCCGGA T^CCGGA
NarI 80 GGCGCC GG^CGCC
NspV 493 TTCGAA TT^CGAA
SacI 2914 GAGCTC GAGCT^C
SapI 2411 GAAGAGC GCTCTTC(1/4)
ScaI 3544 AGTACT AGT^ACT
SfoI 80 GGCGCC GGC^GCC
SfuI 493 TTCGAA TT^CGAA
SmaI 4398 CCCGGG CCC^GGG
SphI 1593 GCATGC GCATG^C
SwaI 2475 ATTTAAAT ATTT^AAAT
XbaI 1972 TCTAGA T^CTAGA
XmaCI 4398 CCCGGG C^CCGGG
XmaI 4398 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 80 GGCGCC G[GA]^CG[CT]C
3925 GACGCC
AflII 416 CTTAAG C^TTAAG
6407 CTTAAG
Alw44I 3207 GTGCAC G^TGCAC
7466 GTGCAC
ApaLI 3207 GTGCAC G^TGCAC
7466 GTGCAC
AvrII 2374 CCTAGG C^CTAGG
2837 CCTAGG
BamHI 1447 GGATCC G^GATCC
7509 GGATCC
BbrPI 3171 CACGTG CAC^GTG
6085 CACGTG
BbsI 2060 GTCTTC GAAGAC(2/6)
6750 GTCTTC
BfrI 416 CTTAAG C^TTAAG
6407 CTTAAG
BlnI 2374 CCTAGG C^CTAGG
2837 CCTAGG
BpuAI 2060 GTCTTC GAAGAC(2/6)
6750 GTCTTC
BsaAI 3171 CACGTG [CT]AC^GT[GA]
6085 CACGTG
BsaHI 80 GGCGCC G[GA]^CG[CT]C
3925 GACGCC
BsmBI 306 GAGACG CGTCTC(1/5)
4325 GAGACG
Bsp1407I 426 TGTACA T^GTACA
6081 TGTACA
BsrGI 426 TGTACA T^GTACA
6081 TGTACA
EcoNI 831 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5271 CCTTTTGAAGG
FauI 77 GCGGG CCCGC(4/6)
7314 GCGGG
Hin1I 80 GGCGCC G[GA]^CG[CT]C
3925 GACGCC
MfeI 2001 CAATTG C^AATTG
7215 CAATTG
MunI 2001 CAATTG C^AATTG
7215 CAATTG
NdeI 2547 CATATG CA^TATG
2995 CATATG
PacI 2350 TTAATTAA TTAAT^TAA
2506 TTAATTAA
PmaCI 3171 CACGTG CAC^GTG
6085 CACGTG
PmlI 3171 CACGTG CAC^GTG
6085 CACGTG
SspBI 426 TGTACA T^GTACA
6081 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AseI
AsnI Asp700I AspHI AspI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglII BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NheI NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmnI