################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII Acc65I AccI AccII AclI AgeI AhdI Aor51HI AscI Asp718I AspEI AviII BsiEI BsiWI BspHI BsrBI BsrFI BssHII Bst1107I BstAPI BstUI BstZ17I Cfr10I CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI KpnI KspI MluI MvnI NaeI NgoMIV NotI NruI NsiI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SgrAI SnaBI SpeI Sse8387I StuI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccIII 5372 TCCGGA T^CCGGA ApaI 3091 GGGCCC GGGCC^C BbeI 80 GGCGCC GGCGC^C BcgI 4410 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4694 GGATAC GTATCC(6/5) BclI 1742 TGATCA T^GATCA BglI 591 GCCCGTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 354 GCTAAGC GC^T[ACGT]AGC Bpu1102I 354 GCTAAGC GC^T[ACGT]AGC BsaBI 5501 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 5372 TCCGGA [AT]^CCGG[AT] BseAI 5372 TCCGGA T^CCGGA BspDI 4158 ATCGAT AT^CGAT BspEI 5372 TCCGGA T^CCGGA BssSI 2626 CTCGTG CACGAG(-5/-1) BstBI 493 TTCGAA TT^CGAA BstEII 2882 GGTGACC G^GT[ACGT]ACC BstPI 2882 GGTGACC G^GT[ACGT]ACC CelII 354 GCTAAGC GC^T[ACGT]AGC ClaI 4158 ATCGAT AT^CGAT DrdI 6955 GACCTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 2882 GGTGACC G^GT[ACGT]ACC FbaI 1742 TGATCA T^GATCA HgaI 3925 GACGC GACGC(5/10) KasI 80 GGCGCC G^GCGCC MamI 5501 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 5372 TCCGGA T^CCGGA NarI 80 GGCGCC GG^CGCC NspV 493 TTCGAA TT^CGAA SacI 2914 GAGCTC GAGCT^C SapI 2411 GAAGAGC GCTCTTC(1/4) ScaI 3544 AGTACT AGT^ACT SfoI 80 GGCGCC GGC^GCC SfuI 493 TTCGAA TT^CGAA SmaI 4398 CCCGGG CCC^GGG SphI 1593 GCATGC GCATG^C SwaI 2475 ATTTAAAT ATTT^AAAT XbaI 1972 TCTAGA T^CTAGA XmaCI 4398 CCCGGG C^CCGGG XmaI 4398 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 80 GGCGCC G[GA]^CG[CT]C 3925 GACGCC AflII 416 CTTAAG C^TTAAG 6407 CTTAAG Alw44I 3207 GTGCAC G^TGCAC 7466 GTGCAC ApaLI 3207 GTGCAC G^TGCAC 7466 GTGCAC AvrII 2374 CCTAGG C^CTAGG 2837 CCTAGG BamHI 1447 GGATCC G^GATCC 7509 GGATCC BbrPI 3171 CACGTG CAC^GTG 6085 CACGTG BbsI 2060 GTCTTC GAAGAC(2/6) 6750 GTCTTC BfrI 416 CTTAAG C^TTAAG 6407 CTTAAG BlnI 2374 CCTAGG C^CTAGG 2837 CCTAGG BpuAI 2060 GTCTTC GAAGAC(2/6) 6750 GTCTTC BsaAI 3171 CACGTG [CT]AC^GT[GA] 6085 CACGTG BsaHI 80 GGCGCC G[GA]^CG[CT]C 3925 GACGCC BsmBI 306 GAGACG CGTCTC(1/5) 4325 GAGACG Bsp1407I 426 TGTACA T^GTACA 6081 TGTACA BsrGI 426 TGTACA T^GTACA 6081 TGTACA EcoNI 831 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5271 CCTTTTGAAGG FauI 77 GCGGG CCCGC(4/6) 7314 GCGGG Hin1I 80 GGCGCC G[GA]^CG[CT]C 3925 GACGCC MfeI 2001 CAATTG C^AATTG 7215 CAATTG MunI 2001 CAATTG C^AATTG 7215 CAATTG NdeI 2547 CATATG CA^TATG 2995 CATATG PacI 2350 TTAATTAA TTAAT^TAA 2506 TTAATTAA PmaCI 3171 CACGTG CAC^GTG 6085 CACGTG PmlI 3171 CACGTG CAC^GTG 6085 CACGTG SspBI 426 TGTACA T^GTACA 6081 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AseI AsnI Asp700I AspHI AspI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglII BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmnI