##################################
# NO CUT ENZYMES ( 37 enzymes) #
##################################
AatII Acc65I AclI AhdI AscI
Asp718I AspEI AviII AvrII BamHI
BlnI BsiWI BspLU11I BssSI Bst1107I
BstBI BstZ17I CpoI Eam1105I FseI
FspI HpaI KpnI MluI NheI
NruI NspV PacI PmeI Psp1406I
PvuI RsrII SalI SbfI SfuI
SnaBI Sse8387I
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 3585 TCCGGA T^CCGGA
AflII 2029 CTTAAG C^TTAAG
AgeI 2811 ACCGGT A^CCGGT
Asp700I 1378 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1625 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 4723 CGAGATCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3355 TGATCA T^GATCA
BfrI 2029 CTTAAG C^TTAAG
BseAI 3585 TCCGGA T^CCGGA
BsmBI 671 CGTCTC CGTCTC(1/5)
BspEI 3585 TCCGGA T^CCGGA
BsrBI 3650 GAGCGG CCGCTC(-3/-3)
BssHII 12 GCGCGC G^CGCGC
DrdI 2373 GACCATACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3355 TGATCA T^GATCA
MroI 3585 TCCGGA T^CCGGA
NaeI 250 GCCGGC GCC^GGC
NgoMIV 250 GCCGGC G^CCGGC
NspI 375 GCATGC [GA]CATG^[CT]
PaeR7I 1838 CTCGAG C^TCGAG
PflFI 1625 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2811 ACCGGT A^CCGGT
PshAI 472 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2639 GAGCTC GAGCT^C
SexAI 572 ACCTGGT A^CC[AT]GGT
SgrAI 181 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 264 CCCGGG CCC^GGG
SpeI 5951 ACTAGT A^CTAGT
SphI 375 GCATGC GCATG^C
SspI 7842 AATATT AAT^ATT
Tth111I 1625 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1838 CTCGAG C^TCGAG
XmaCI 264 CCCGGG C^CCGGG
XmaI 264 CCCGGG C^CCGGG
XmnI 1378 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 46 enzymes) #
###################################
AccI 1166 GTAGAC GT^[AC][GT]AC
7088 GTAGAC
AflIII 426 ACACGT A^C[GA][CT]GT
428 ACGTGT
Alw44I 343 GTGCAC G^TGCAC
2599 GTGCAC
AocI 3185 CCTGAGG CC^T[ACGT]AGG
5986 CCTAAGG
ApaI 121 GGGCCC GGGCC^C
734 GGGCCC
ApaLI 343 GTGCAC G^TGCAC
2599 GTGCAC
BbeI 56 GGCGCC GGCGC^C
1352 GGCGCC
BbrPI 427 CACGTG CAC^GTG
2954 CACGTG
BbvCI 4765 CCTCAGC CCTCAGC(-5/-2)
6352 CCTCAGC
BlpI 4040 GCTCAGC GC^T[ACGT]AGC
4045 GCTGAGC
Bpu1102I 4040 GCTCAGC GC^T[ACGT]AGC
4045 GCTGAGC
BsaBI 3658 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6266 GATTTAAATC
BsaWI 2811 ACCGGT [AT]^CCGG[AT]
3585 TCCGGA
Bsp1407I 1736 TGTACA T^GTACA
5341 TGTACA
BspDI 2726 ATCGAT AT^CGAT
5412 ATCGAT
BspHI 4971 TCATGA T^CATGA
6577 TCATGA
BsrGI 1736 TGTACA T^GTACA
5341 TGTACA
BstEII 469 GGTGACC G^GT[ACGT]ACC
478 GGTCACC
BstPI 469 GGTGACC G^GT[ACGT]ACC
478 GGTCACC
Bsu36I 3185 CCTGAGG CC^T[ACGT]AGG
5986 CCTAAGG
CelII 4040 GCTCAGC GC^T[ACGT]AGC
4045 GCTGAGC
ClaI 2726 ATCGAT AT^CGAT
5412 ATCGAT
Eco81I 3185 CCTGAGG CC^T[ACGT]AGG
5986 CCTAAGG
EcoO65I 469 GGTGACC G^GT[ACGT]ACC
478 GGTCACC
EcoRI 848 GAATTC G^AATTC
6708 GAATTC
EcoRV 1862 GATATC GAT^ATC
4958 GATATC
EcoT22I 5526 ATGCAT ATGCA^T
7323 ATGCAT
HindIII 648 AAGCTT A^AGCTT
3779 AAGCTT
KasI 56 GGCGCC G^GCGCC
1352 GGCGCC
KspI 309 CCGCGG CCGC^GG
796 CCGCGG
MamI 3658 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6266 GATTTAAATC
NarI 56 GGCGCC GG^CGCC
1352 GGCGCC
NdeI 1158 CATATG CA^TATG
7320 CATATG
NotI 302 GCGGCCGC GC^GGCCGC
361 GCGGCCGC
NsiI 5526 ATGCAT ATGCA^T
7323 ATGCAT
PmaCI 427 CACGTG CAC^GTG
2954 CACGTG
PmlI 427 CACGTG CAC^GTG
2954 CACGTG
RcaI 4971 TCATGA T^CATGA
6577 TCATGA
SacII 309 CCGCGG CCGC^GG
796 CCGCGG
SapI 3914 GAAGAGC GCTCTTC(1/4)
1410 GCTCTTC
ScaI 753 AGTACT AGT^ACT
5441 AGTACT
SfoI 56 GGCGCC GGC^GCC
1352 GGCGCC
SspBI 1736 TGTACA T^GTACA
5341 TGTACA
SwaI 5785 ATTTAAAT ATTT^AAAT
6267 ATTTAAAT
XbaI 6624 TCTAGA T^CTAGA
6704 TCTAGA
XcmI 751 CCAGTACTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3139 CCACTTTGGGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApoI
AseI AsnI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BciVI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII