##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Aor51HI ApaI AscI Asp718I AviII
BalI BbeI BcgI BglI BlpI
Bpu1102I BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I FseI FspI Hin1I
KasI KpnI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PpuMI PvuI RsrII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AhdI 2061 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2061 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3605 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 168 CCCGGG C^[CT]CG[GA]G
BbrPI 3538 CACGTG CAC^GTG
BciVI 356 GGATAC GTATCC(6/5)
BsmI 570 GAATGC GAATGC(1/-1)
BsoBI 168 CCCGGG C^[CT]CG[GA]G
BspMI 58 ACCTGC ACCTGC(4/8)
BsrFI 5653 ACCGGC [GA]^CCGG[CT]
BstAPI 1425 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2575 GGTAACC G^GT[ACGT]ACC
BstPI 2575 GGTAACC G^GT[ACGT]ACC
BstXI 1593 CCAGTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 5653 ACCGGC [GA]^CCGG[CT]
Eam1105I 2061 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2575 GGTAACC G^GT[ACGT]ACC
HgaI 4001 GCGTC GACGC(5/10)
HpaI 5511 GTTAAC GTT^AAC
KspI 100 CCGCGG CCGC^GG
PflFI 3605 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3538 CACGTG CAC^GTG
PmlI 3538 CACGTG CAC^GTG
PshAI 5724 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 100 CCGCGG CCGC^GG
SapI 1113 GAAGAGC GCTCTTC(1/4)
ScaI 5013 AGTACT AGT^ACT
SmaI 168 CCCGGG CCC^GGG
SpeI 3038 ACTAGT A^CTAGT
SphI 4198 GCATGC GCATG^C
Tth111I 3605 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 168 CCCGGG C^CCGGG
XmaI 168 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 833 GTCTAC GT^[AC][GT]AC
5945 GTCTAC
AclI 1986 AACGTT AA^CGTT
6445 AACGTT
AflII 3879 CTTAAG C^TTAAG
5168 CTTAAG
Alw44I 189 GTGCAC G^TGCAC
2918 GTGCAC
ApaLI 189 GTGCAC G^TGCAC
2918 GTGCAC
AseI 1158 ATTAAT AT^TAAT
4068 ATTAAT
AsnI 1158 ATTAAT AT^TAAT
4068 ATTAAT
AvrII 1578 CCTAGG C^CTAGG
3981 CCTAGG
BamHI 1195 GGATCC G^GATCC
4767 GGATCC
BbsI 6779 GAAGAC GAAGAC(2/6)
2886 GTCTTC
BbvCI 6697 CCTCAGC CCTCAGC(-5/-2)
6506 GCTGAGG
BfrI 3879 CTTAAG C^TTAAG
5168 CTTAAG
BlnI 1578 CCTAGG C^CTAGG
3981 CCTAGG
BpuAI 6779 GAAGAC GAAGAC(2/6)
2886 GTCTTC
BsaBI 3962 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5884 GATTTGCATC
BspHI 2788 TCATGA T^CATGA
6769 TCATGA
BssSI 4821 CTCGTG CACGAG(-5/-1)
6433 CTCGTG
DraII 211 AGGGCCT [GA]G^G[ACGT]CC[CT]
623 GGGGCCT
DsaI 100 CCGCGG C^C[GA][CT]GG
6622 CCGTGG
EcoO109I 211 AGGGCCT [GA]G^G[ACGT]CC[CT]
623 GGGGCCT
EcoT22I 4169 ATGCAT ATGCA^T
4570 ATGCAT
FauI 35 GCGGG CCCGC(4/6)
3747 GCGGG
HaeII 195 AGCGCC [GA]GCGC^[CT]
5615 GGCGCT
HincII 579 GTCAAC GT[CT]^[GA]AC
5511 GTTAAC
HindII 579 GTCAAC GT[CT]^[GA]AC
5511 GTTAAC
MamI 3962 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5884 GATTTGCATC
NdeI 2399 CATATG CA^TATG
5585 CATATG
NsiI 4169 ATGCAT ATGCA^T
4570 ATGCAT
PflMI 861 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4007 CCACTCTGTGG
PshBI 1158 ATTAAT AT^TAAT
4068 ATTAAT
Psp1406I 1986 AACGTT AA^CGTT
6445 AACGTT
RcaI 2788 TCATGA T^CATGA
6769 TCATGA
StuI 3978 AGGCCT AGG^CCT
4817 AGGCCT
Van91I 861 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4007 CCACTCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BanI BanII
BbvI BclI BcnI BfaI BglII
BmyI BpmI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraIII EaeI EarI Eco81I EcoNI
EcoRI EcoRII EcoRV EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII XmnI