################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII Acc65I AccIII AcyI AgeI Aor51HI ApaI AscI Asp718I AviII BalI BbeI BcgI BglI BlpI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI DrdI EagI EclXI Eco47III Eco52I FseI FspI Hin1I KasI KpnI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PpuMI PvuI RsrII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AhdI 2061 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 2061 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3605 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 168 CCCGGG C^[CT]CG[GA]G BbrPI 3538 CACGTG CAC^GTG BciVI 356 GGATAC GTATCC(6/5) BsmI 570 GAATGC GAATGC(1/-1) BsoBI 168 CCCGGG C^[CT]CG[GA]G BspMI 58 ACCTGC ACCTGC(4/8) BsrFI 5653 ACCGGC [GA]^CCGG[CT] BstAPI 1425 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2575 GGTAACC G^GT[ACGT]ACC BstPI 2575 GGTAACC G^GT[ACGT]ACC BstXI 1593 CCAGTGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 5653 ACCGGC [GA]^CCGG[CT] Eam1105I 2061 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 2575 GGTAACC G^GT[ACGT]ACC HgaI 4001 GCGTC GACGC(5/10) HpaI 5511 GTTAAC GTT^AAC KspI 100 CCGCGG CCGC^GG PflFI 3605 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3538 CACGTG CAC^GTG PmlI 3538 CACGTG CAC^GTG PshAI 5724 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 100 CCGCGG CCGC^GG SapI 1113 GAAGAGC GCTCTTC(1/4) ScaI 5013 AGTACT AGT^ACT SmaI 168 CCCGGG CCC^GGG SpeI 3038 ACTAGT A^CTAGT SphI 4198 GCATGC GCATG^C Tth111I 3605 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 168 CCCGGG C^CCGGG XmaI 168 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 833 GTCTAC GT^[AC][GT]AC 5945 GTCTAC AclI 1986 AACGTT AA^CGTT 6445 AACGTT AflII 3879 CTTAAG C^TTAAG 5168 CTTAAG Alw44I 189 GTGCAC G^TGCAC 2918 GTGCAC ApaLI 189 GTGCAC G^TGCAC 2918 GTGCAC AseI 1158 ATTAAT AT^TAAT 4068 ATTAAT AsnI 1158 ATTAAT AT^TAAT 4068 ATTAAT AvrII 1578 CCTAGG C^CTAGG 3981 CCTAGG BamHI 1195 GGATCC G^GATCC 4767 GGATCC BbsI 6779 GAAGAC GAAGAC(2/6) 2886 GTCTTC BbvCI 6697 CCTCAGC CCTCAGC(-5/-2) 6506 GCTGAGG BfrI 3879 CTTAAG C^TTAAG 5168 CTTAAG BlnI 1578 CCTAGG C^CTAGG 3981 CCTAGG BpuAI 6779 GAAGAC GAAGAC(2/6) 2886 GTCTTC BsaBI 3962 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5884 GATTTGCATC BspHI 2788 TCATGA T^CATGA 6769 TCATGA BssSI 4821 CTCGTG CACGAG(-5/-1) 6433 CTCGTG DraII 211 AGGGCCT [GA]G^G[ACGT]CC[CT] 623 GGGGCCT DsaI 100 CCGCGG C^C[GA][CT]GG 6622 CCGTGG EcoO109I 211 AGGGCCT [GA]G^G[ACGT]CC[CT] 623 GGGGCCT EcoT22I 4169 ATGCAT ATGCA^T 4570 ATGCAT FauI 35 GCGGG CCCGC(4/6) 3747 GCGGG HaeII 195 AGCGCC [GA]GCGC^[CT] 5615 GGCGCT HincII 579 GTCAAC GT[CT]^[GA]AC 5511 GTTAAC HindII 579 GTCAAC GT[CT]^[GA]AC 5511 GTTAAC MamI 3962 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5884 GATTTGCATC NdeI 2399 CATATG CA^TATG 5585 CATATG NsiI 4169 ATGCAT ATGCA^T 4570 ATGCAT PflMI 861 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4007 CCACTCTGTGG PshBI 1158 ATTAAT AT^TAAT 4068 ATTAAT Psp1406I 1986 AACGTT AA^CGTT 6445 AACGTT RcaI 2788 TCATGA T^CATGA 6769 TCATGA StuI 3978 AGGCCT AGG^CCT 4817 AGGCCT Van91I 861 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4007 CCACTCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI Asp700I AspHI AvaII BanI BanII BbvI BclI BcnI BfaI BglII BmyI BpmI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraIII EaeI EarI Eco81I EcoNI EcoRI EcoRII EcoRV EcoT14I FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI