##################################
# NO CUT ENZYMES ( 39 enzymes) #
##################################
AclI AflII Alw44I ApaLI AscI
AvrII BbrPI BfrI BlnI BsiWI
BssHII Bst1107I BstZ17I CpoI FseI
HindIII KspI MfeI MluI MunI
NaeI NgoMIV NotI NruI PacI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SgrAI SnaBI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 5963 GACGTC GACGT^C
AccIII 6918 TCCGGA T^CCGGA
AgeI 44 ACCGGT A^CCGGT
AhdI 3625 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 7531 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3625 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2291 TGCGCA TGC^GCA
BbeI 3412 GGCGCC GGCGC^C
BseAI 6918 TCCGGA T^CCGGA
BspDI 5233 ATCGAT AT^CGAT
BspEI 6918 TCCGGA T^CCGGA
BsrBI 1481 GAGCGG CCGCTC(-3/-3)
BstEII 5187 GGTGACC G^GT[ACGT]ACC
BstPI 5187 GGTGACC G^GT[ACGT]ACC
ClaI 5233 ATCGAT AT^CGAT
DraI 8693 TTTAAA TTT^AAA
Eam1105I 3625 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5187 GGTGACC G^GT[ACGT]ACC
EcoRV 1600 GATATC GAT^ATC
FspI 2291 TGCGCA TGC^GCA
HpaI 6715 GTTAAC GTT^AAC
KasI 3412 GGCGCC G^GCGCC
MroI 6918 TCCGGA T^CCGGA
NarI 3412 GGCGCC GG^CGCC
NheI 373 GCTAGC G^CTAGC
PinAI 44 ACCGGT A^CCGGT
PshAI 5989 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 3412 GGCGCC GGC^GCC
SmaI 2828 CCCGGG CCC^GGG
SpeI 2893 ACTAGT A^CTAGT
SspI 7855 AATATT AAT^ATT
XmaCI 2828 CCCGGG C^CCGGG
XmaI 2828 CCCGGG C^CCGGG
XmnI 7531 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 41 GGTACC G^GTACC
5999 GGTACC
AccI 279 GTCTAC GT^[AC][GT]AC
6045 GTCTAC
AflIII 5210 ACATGT A^C[GA][CT]GT
6092 ACATGT
AseI 2436 ATTAAT AT^TAAT
4357 ATTAAT
AsnI 2436 ATTAAT AT^TAAT
4357 ATTAAT
Asp718I 41 GGTACC G^GTACC
5999 GGTACC
AspI 3528 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7336 GACAGTGTC
BcgI 776 CGAAGGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2072 GCAAACTGCTCG
BciVI 3757 GGATAC GTATCC(6/5)
5805 GGATAC
BclI 1212 TGATCA T^GATCA
2483 TGATCA
BsaAI 532 TACGTG [CT]AC^GT[GA]
7994 CACGTA
Bsp1407I 1065 TGTACA T^GTACA
5738 TGTACA
BspLU11I 5210 ACATGT A^CATGT
6092 ACATGT
BsrFI 44 ACCGGT [GA]^CCGG[CT]
1178 GCCGGT
BsrGI 1065 TGTACA T^GTACA
5738 TGTACA
BssSI 4462 CACGAG CACGAG(-5/-1)
5786 CACGAG
BstBI 3581 TTCGAA TT^CGAA
5494 TTCGAA
Cfr10I 44 ACCGGT [GA]^CCGG[CT]
1178 GCCGGT
DraIII 2797 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
7303 CACACAGTG
DrdI 457 GACCCAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1537 GACGCCACAGTC
EagI 4388 CGGCCG C^GGCCG
5603 CGGCCG
EclXI 4388 CGGCCG C^GGCCG
5603 CGGCCG
Eco52I 4388 CGGCCG C^GGCCG
5603 CGGCCG
EcoRI 3220 GAATTC G^AATTC
4186 GAATTC
EcoT22I 3349 ATGCAT ATGCA^T
3830 ATGCAT
FbaI 1212 TGATCA T^GATCA
2483 TGATCA
KpnI 41 GGTACC GGTAC^C
5999 GGTACC
NsiI 3349 ATGCAT ATGCA^T
3830 ATGCAT
NspV 3581 TTCGAA TT^CGAA
5494 TTCGAA
PaeR7I 1571 CTCGAG C^TCGAG
4552 CTCGAG
PflFI 3528 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7336 GACAGTGTC
PshBI 2436 ATTAAT AT^TAAT
4357 ATTAAT
SapI 3171 GAAGAGC GCTCTTC(1/4)
6702 GCTCTTC
SfuI 3581 TTCGAA TT^CGAA
5494 TTCGAA
SspBI 1065 TGTACA T^GTACA
5738 TGTACA
Tth111I 3528 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
7336 GACAGTGTC
XbaI 4338 TCTAGA T^CTAGA
8638 TCTAGA
XhoI 1571 CTCGAG C^TCGAG
4552 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI Aor51HI
ApaI ApoI AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScaI ScrFI SexAI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII