##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AspI
AviII BbrPI BcgI BfrI BlpI
Bpu1102I BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BspDI
BspEI BsrFI BssHII BstAPI BstBI
Bsu36I CelII Cfr10I ClaI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoT22I FseI
FspI HincII HindII HpaI MfeI
MluI MroI MunI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI SalI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmlI Sse8387I SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 2710 GGTACC G^GTACC
AflIII 2357 ACATGT A^C[GA][CT]GT
AlwNI 2154 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 787 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2710 GGTACC G^GTACC
AvrII 62 CCTAGG C^CTAGG
BalI 256 TGGCCA TGG^CCA
BbeI 66 GGCGCC GGCGC^C
BbvCI 1404 GCTGAGG CCTCAGC(-5/-2)
BclI 1613 TGATCA T^GATCA
BglI 231 GCCGCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 628 AGATCT A^GATCT
BlnI 62 CCTAGG C^CTAGG
BsaBI 884 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 2156 GAGAC GTCTC(1/5)
BspHI 1127 TCATGA T^CATGA
BspLU11I 2357 ACATGT A^CATGT
BsrBI 118 CCGCTC CCGCTC(-3/-3)
BssSI 576 CTCGTG CACGAG(-5/-1)
BstEII 921 GGTTACC G^GT[ACGT]ACC
BstPI 921 GGTTACC G^GT[ACGT]ACC
CpoI 25 CGGACCG CG^G[AT]CCG
DraIII 1376 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 256 TGGCCA [CT]^GGCC[GA]
EcoO65I 921 GGTTACC G^GT[ACGT]ACC
EcoRV 2686 GATATC GAT^ATC
FbaI 1613 TGATCA T^GATCA
HindIII 935 AAGCTT A^AGCTT
KasI 66 GGCGCC G^GCGCC
KpnI 2710 GGTACC GGTAC^C
MamI 884 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 256 TGGCCA TGG^CCA
MscI 256 TGGCCA TGG^CCA
NarI 66 GGCGCC GG^CGCC
NdeI 721 CATATG CA^TATG
PflMI 1271 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1163 CAGCTG CAG^CTG
RcaI 1127 TCATGA T^CATGA
RsrII 25 CGGACCG CG^G[AT]CCG
SacI 106 GAGCTC GAGCT^C
SapI 972 GAAGAGC GCTCTTC(1/4)
SfoI 66 GGCGCC GGC^GCC
SmaI 449 CCCGGG CCC^GGG
SnaBI 2820 TACGTA TAC^GTA
SpeI 612 ACTAGT A^CTAGT
SphI 899 GCATGC GCATG^C
Tsp45I 2342 GTCAC ^GT[GC]AC
Van91I 1271 CCATGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1448 CCAGGGAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 449 CCCGGG C^CCGGG
XmaI 449 CCCGGG C^CCGGG
XmnI 787 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 19 GGGCCC GGGCC^C
445 GGGCCC
AspHI 106 GAGCTC G[AT]GC[AT]^C
1433 GTGCTC
BamHI 588 GGATCC G^GATCC
707 GGATCC
BanI 66 GGCGCC G^G[CT][GA]CC
2710 GGTACC
BbsI 1530 GAAGAC GAAGAC(2/6)
2032 GTCTTC
BciVI 697 GGATAC GTATCC(6/5)
864 GGATAC
BpuAI 1530 GAAGAC GAAGAC(2/6)
2032 GTCTTC
BsaAI 1980 TACGTG [CT]AC^GT[GA]
2820 TACGTA
BsiHKAI 106 GAGCTC G[AT]GC[AT]^C
1433 GTGCTC
Bsp1407I 2227 TGTACA T^GTACA
2641 TGTACA
BspMI 313 GCAGGT ACCTGC(4/8)
2028 GCAGGT
BsrGI 2227 TGTACA T^GTACA
2641 TGTACA
Bst1107I 1554 GTATAC GTA^TAC
1670 GTATAC
BstXI 259 CCAGACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1491 CCAGATAAGTGG
BstZ17I 1554 GTATAC GTA^TAC
1670 GTATAC
EarI 972 GAAGAG CTCTTC(1/4)
2281 GAAGAG
FauI 117 CCCGC CCCGC(4/6)
192 CCCGC
Ksp632I 972 GAAGAG CTCTTC(1/4)
2281 GAAGAG
KspI 32 CCGCGG CCGC^GG
193 CCGCGG
SacII 32 CCGCGG CCGC^GG
193 CCGCGG
SspBI 2227 TGTACA T^GTACA
2641 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI AvaI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BsiYI
BslI BsmFI BsmI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII