################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp718I AspEI AviII BbrPI BcgI BciVI BclI BfrI BglII BlpI Bpu1102I BsiEI BsiWI BsmBI Bsp1407I BspDI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI HgaI KpnI KspI MfeI MluI MspA1I MunI NdeI NheI NotI NruI NspV PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccIII 235 TCCGGA T^CCGGA AcyI 71 GGCGCC G[GA]^CG[CT]C AflIII 1698 ACATGT A^C[GA][CT]GT AocI 1587 CCTGAGG CC^T[ACGT]AGG Asp700I 500 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 3666 GAGCTC G[AT]GC[AT]^C AspI 2063 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1742 GGATCC G^GATCC BanII 3666 GAGCTC G[GA]GC[CT]^C BbeI 71 GGCGCC GGCGC^C BbsI 3883 GTCTTC GAAGAC(2/6) BglI 7 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 3883 GTCTTC GAAGAC(2/6) BsaAI 2746 CACGTA [CT]AC^GT[GA] BsaBI 2575 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 71 GGCGCC G[GA]^CG[CT]C BseAI 235 TCCGGA T^CCGGA BsiHKAI 3666 GAGCTC G[AT]GC[AT]^C BsmI 2522 GCATTC GAATGC(1/-1) BspEI 235 TCCGGA T^CCGGA BspHI 3269 TCATGA T^CATGA BspLU11I 1698 ACATGT A^CATGT BsrDI 733 GCAATG GCAATG(2/0) BstAPI 3141 GCAATTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1587 CCTGAGG CC^T[ACGT]AGG DsaI 3218 CCATGG C^C[GA][CT]GG Eco81I 1587 CCTGAGG CC^T[ACGT]AGG EcoNI 3153 CCTAGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Hin1I 71 GGCGCC G[GA]^CG[CT]C HindIII 3924 AAGCTT A^AGCTT HpaI 3867 GTTAAC GTT^AAC KasI 71 GGCGCC G^GCGCC MamI 2575 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 235 TCCGGA T^CCGGA NarI 71 GGCGCC GG^CGCC NcoI 3218 CCATGG C^CATGG NspI 1698 ACATGT [GA]CATG^[CT] PacI 2850 TTAATTAA TTAAT^TAA PaeR7I 1855 CTCGAG C^TCGAG PflFI 2063 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 604 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 293 GGGTCCC [GA]G^G[AT]CC[CT] RcaI 3269 TCATGA T^CATGA SacI 3666 GAGCTC GAGCT^C ScaI 1081 AGTACT AGT^ACT SfoI 71 GGCGCC GGC^GCC SgrAI 46 CGCCGGCG C[GA]^CCGG[CT]G SmaI 2381 CCCGGG CCC^GGG Tth111I 2063 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 604 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3359 TCTAGA T^CTAGA XhoI 1855 CTCGAG C^TCGAG XmaCI 2381 CCCGGG C^CCGGG XmaI 2381 CCCGGG C^CCGGG XmnI 500 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AvrII 3153 CCTAGG C^CTAGG 3654 CCTAGG BalI 2181 TGGCCA TGG^CCA 3482 TGGCCA BanI 71 GGCGCC G^G[CT][GA]CC 3238 GGTGCC BbvCI 1972 GCTGAGG CCTCAGC(-5/-2) 3597 GCTGAGG BcnI 2381 CCCGG CC^[GC]GG 2382 CCGGG BlnI 3153 CCTAGG C^CTAGG 3654 CCTAGG BsaI 1167 GAGACC GGTCTC(1/5) 2592 GAGACC BsaWI 235 TCCGGA [AT]^CCGG[AT] 1799 TCCGGT BsrFI 7 GCCGGC [GA]^CCGG[CT] 47 GCCGGC BstYI 1593 GGATCT [GA]^GATC[CT] 1742 GGATCC Cfr10I 7 GCCGGC [GA]^CCGG[CT] 47 GCCGGC DraII 293 GGGTCCC [GA]G^G[ACGT]CC[CT] 2376 AGGCCCC EcoO109I 293 GGGTCCC [GA]G^G[ACGT]CC[CT] 2376 AGGCCCC EcoT22I 346 ATGCAT ATGCA^T 397 ATGCAT HincII 1621 GTCAAC GT[CT]^[GA]AC 3867 GTTAAC HindII 1621 GTCAAC GT[CT]^[GA]AC 3867 GTTAAC MaeII 825 ACGT A^CGT 2747 ACGT MflI 1593 GGATCT [GA]^GATC[CT] 1742 GGATCC MluNI 2181 TGGCCA TGG^CCA 3482 TGGCCA MscI 2181 TGGCCA TGG^CCA 3482 TGGCCA NaeI 7 GCCGGC GCC^GGC 47 GCCGGC NciI 2381 CCCGG CC^[GC]GG 2382 CCGGG NgoMIV 7 GCCGGC G^CCGGC 47 GCCGGC NsiI 346 ATGCAT ATGCA^T 397 ATGCAT TaqI 1856 TCGA T^CGA 2137 TCGA TthHB8I 1856 TCGA T^CGA 2137 TCGA XcmI 1338 CCATTTGTTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3783 CCAGTGTCAGGATGG XhoII 1593 GGATCT [GA]^GATC[CT] 1742 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AseI AsnI AvaI AvaII BbvI BfaI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI