##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AviII BbrPI BcgI BciVI BclI
BfrI BglII BlpI Bpu1102I BsiEI
BsiWI BsmBI Bsp1407I BspDI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI HgaI
KpnI KspI MfeI MluI MspA1I
MunI NdeI NheI NotI NruI
NspV PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SapI SbfI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccIII 235 TCCGGA T^CCGGA
AcyI 71 GGCGCC G[GA]^CG[CT]C
AflIII 1698 ACATGT A^C[GA][CT]GT
AocI 1587 CCTGAGG CC^T[ACGT]AGG
Asp700I 500 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 3666 GAGCTC G[AT]GC[AT]^C
AspI 2063 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1742 GGATCC G^GATCC
BanII 3666 GAGCTC G[GA]GC[CT]^C
BbeI 71 GGCGCC GGCGC^C
BbsI 3883 GTCTTC GAAGAC(2/6)
BglI 7 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 3883 GTCTTC GAAGAC(2/6)
BsaAI 2746 CACGTA [CT]AC^GT[GA]
BsaBI 2575 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 71 GGCGCC G[GA]^CG[CT]C
BseAI 235 TCCGGA T^CCGGA
BsiHKAI 3666 GAGCTC G[AT]GC[AT]^C
BsmI 2522 GCATTC GAATGC(1/-1)
BspEI 235 TCCGGA T^CCGGA
BspHI 3269 TCATGA T^CATGA
BspLU11I 1698 ACATGT A^CATGT
BsrDI 733 GCAATG GCAATG(2/0)
BstAPI 3141 GCAATTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1587 CCTGAGG CC^T[ACGT]AGG
DsaI 3218 CCATGG C^C[GA][CT]GG
Eco81I 1587 CCTGAGG CC^T[ACGT]AGG
EcoNI 3153 CCTAGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 71 GGCGCC G[GA]^CG[CT]C
HindIII 3924 AAGCTT A^AGCTT
HpaI 3867 GTTAAC GTT^AAC
KasI 71 GGCGCC G^GCGCC
MamI 2575 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 235 TCCGGA T^CCGGA
NarI 71 GGCGCC GG^CGCC
NcoI 3218 CCATGG C^CATGG
NspI 1698 ACATGT [GA]CATG^[CT]
PacI 2850 TTAATTAA TTAAT^TAA
PaeR7I 1855 CTCGAG C^TCGAG
PflFI 2063 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 604 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 293 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 3269 TCATGA T^CATGA
SacI 3666 GAGCTC GAGCT^C
ScaI 1081 AGTACT AGT^ACT
SfoI 71 GGCGCC GGC^GCC
SgrAI 46 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2381 CCCGGG CCC^GGG
Tth111I 2063 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 604 CCAAATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3359 TCTAGA T^CTAGA
XhoI 1855 CTCGAG C^TCGAG
XmaCI 2381 CCCGGG C^CCGGG
XmaI 2381 CCCGGG C^CCGGG
XmnI 500 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AvrII 3153 CCTAGG C^CTAGG
3654 CCTAGG
BalI 2181 TGGCCA TGG^CCA
3482 TGGCCA
BanI 71 GGCGCC G^G[CT][GA]CC
3238 GGTGCC
BbvCI 1972 GCTGAGG CCTCAGC(-5/-2)
3597 GCTGAGG
BcnI 2381 CCCGG CC^[GC]GG
2382 CCGGG
BlnI 3153 CCTAGG C^CTAGG
3654 CCTAGG
BsaI 1167 GAGACC GGTCTC(1/5)
2592 GAGACC
BsaWI 235 TCCGGA [AT]^CCGG[AT]
1799 TCCGGT
BsrFI 7 GCCGGC [GA]^CCGG[CT]
47 GCCGGC
BstYI 1593 GGATCT [GA]^GATC[CT]
1742 GGATCC
Cfr10I 7 GCCGGC [GA]^CCGG[CT]
47 GCCGGC
DraII 293 GGGTCCC [GA]G^G[ACGT]CC[CT]
2376 AGGCCCC
EcoO109I 293 GGGTCCC [GA]G^G[ACGT]CC[CT]
2376 AGGCCCC
EcoT22I 346 ATGCAT ATGCA^T
397 ATGCAT
HincII 1621 GTCAAC GT[CT]^[GA]AC
3867 GTTAAC
HindII 1621 GTCAAC GT[CT]^[GA]AC
3867 GTTAAC
MaeII 825 ACGT A^CGT
2747 ACGT
MflI 1593 GGATCT [GA]^GATC[CT]
1742 GGATCC
MluNI 2181 TGGCCA TGG^CCA
3482 TGGCCA
MscI 2181 TGGCCA TGG^CCA
3482 TGGCCA
NaeI 7 GCCGGC GCC^GGC
47 GCCGGC
NciI 2381 CCCGG CC^[GC]GG
2382 CCGGG
NgoMIV 7 GCCGGC G^CCGGC
47 GCCGGC
NsiI 346 ATGCAT ATGCA^T
397 ATGCAT
TaqI 1856 TCGA T^CGA
2137 TCGA
TthHB8I 1856 TCGA T^CGA
2137 TCGA
XcmI 1338 CCATTTGTTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3783 CCAGTGTCAGGATGG
XhoII 1593 GGATCT [GA]^GATC[CT]
1742 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AvaI
AvaII BbvI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI