##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AatII AgeI AhdI Aor51HI AspEI
AspI AviII BbrPI BsaBI BsiEI
BspHI BssSI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
MamI MluI NaeI NdeI NgoMIV
NotI NruI PacI PflFI PinAI
PmaCI PmeI PmlI PvuI RcaI
SbfI ScaI SexAI SfiI SgrAI
SpeI Sse8387I Tth111I
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AflII 5677 CTTAAG C^TTAAG
AscI 530 GGCGCGCC GG^CGCGCC
AseI 5596 ATTAAT AT^TAAT
AsnI 5596 ATTAAT AT^TAAT
AvrII 6670 CCTAGG C^CTAGG
BcgI 814 GCACGCACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 5677 CTTAAG C^TTAAG
BlnI 6670 CCTAGG C^CTAGG
BsiWI 2271 CGTACG C^GTACG
BsmBI 280 CGTCTC CGTCTC(1/5)
BspDI 1327 ATCGAT AT^CGAT
BsrFI 167 ACCGGC [GA]^CCGG[CT]
BstBI 3263 TTCGAA TT^CGAA
Cfr10I 167 ACCGGC [GA]^CCGG[CT]
ClaI 1327 ATCGAT AT^CGAT
CpoI 412 CGGACCG CG^G[AT]CCG
DrdI 3565 GACTTACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 3680 AAGCTT A^AGCTT
HpaI 4546 GTTAAC GTT^AAC
KspI 148 CCGCGG CCGC^GG
NheI 1201 GCTAGC G^CTAGC
NspV 3263 TTCGAA TT^CGAA
PaeR7I 2496 CTCGAG C^TCGAG
PshBI 5596 ATTAAT AT^TAAT
RsrII 412 CGGACCG CG^G[AT]CCG
SacI 4877 GAGCTC GAGCT^C
SacII 148 CCGCGG CCGC^GG
SalI 669 GTCGAC G^TCGAC
SfuI 3263 TTCGAA TT^CGAA
SnaBI 5062 TACGTA TAC^GTA
SwaI 5324 ATTTAAAT ATTT^AAAT
XhoI 2496 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 1819 GGTACC G^GTACC
2086 GGTACC
AccIII 1359 TCCGGA T^CCGGA
4488 TCCGGA
AclI 5207 AACGTT AA^CGTT
5801 AACGTT
AflIII 3001 ACATGT A^C[GA][CT]GT
3570 ACATGT
AlwNI 4468 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
7202 CAGCATCTG
Asp718I 1819 GGTACC G^GTACC
2086 GGTACC
BbeI 114 GGCGCC GGCGC^C
209 GGCGCC
BbvCI 1365 CCTCAGC CCTCAGC(-5/-2)
885 GCTGAGG
BclI 4697 TGATCA T^GATCA
6414 TGATCA
BglI 269 GCCTGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3803 GCCTTGGGGGC
BglII 2776 AGATCT A^GATCT
2784 AGATCT
BsaAI 78 CACGTA [CT]AC^GT[GA]
5062 TACGTA
BseAI 1359 TCCGGA T^CCGGA
4488 TCCGGA
BspEI 1359 TCCGGA T^CCGGA
4488 TCCGGA
BspLU11I 3001 ACATGT A^CATGT
3570 ACATGT
Bst1107I 5232 GTATAC GTA^TAC
5351 GTATAC
BstAPI 1474 GCAACTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
7201 GCAGCATCTGC
BstEII 322 GGTGACC G^GT[ACGT]ACC
2395 GGTAACC
BstPI 322 GGTGACC G^GT[ACGT]ACC
2395 GGTAACC
BstZ17I 5232 GTATAC GTA^TAC
5351 GTATAC
EcoO65I 322 GGTGACC G^GT[ACGT]ACC
2395 GGTAACC
FbaI 4697 TGATCA T^GATCA
6414 TGATCA
HaeII 114 GGCGCC [GA]GCGC^[CT]
209 GGCGCC
KasI 114 GGCGCC G^GCGCC
209 GGCGCC
KpnI 1819 GGTACC GGTAC^C
2086 GGTACC
MfeI 1928 CAATTG C^AATTG
4309 CAATTG
MroI 1359 TCCGGA T^CCGGA
4488 TCCGGA
MunI 1928 CAATTG C^AATTG
4309 CAATTG
NarI 114 GGCGCC GG^CGCC
209 GGCGCC
PflMI 2047 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3744 CCAGCTGGTGG
PshAI 1351 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1534 GACCTGAGTC
Psp1406I 5207 AACGTT AA^CGTT
5801 AACGTT
SapI 522 GAAGAGC GCTCTTC(1/4)
968 GCTCTTC
SfoI 114 GGCGCC GGC^GCC
209 GGCGCC
SphI 2387 GCATGC GCATG^C
6221 GCATGC
StuI 3529 AGGCCT AGG^CCT
4755 AGGCCT
Van91I 2047 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3744 CCAGCTGGTGG
XbaI 5773 TCTAGA T^CTAGA
6918 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AocI
ApaI ApaLI ApoI Asp700I AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI XmnI