##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AccIII AclI AhdI
AscI Asp718I AspEI AspI AviII
BamHI BciVI BglI BseAI BsiWI
BspDI BspEI BspHI BssHII Bst1107I
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I FseI FspI
KpnI KspI MluI MroI NaeI
NgoMIV NotI NruI PacI PaeR7I
PflFI PmeI Psp1406I RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SgrAI SpeI SphI
Sse8387I SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 113 GGCGCC G[GA]^CG[CT]C
AgeI 684 ACCGGT A^CCGGT
Aor51HI 26 AGCGCT AGC^GCT
ApaI 6477 GGGCCC GGGCC^C
AvrII 3147 CCTAGG C^CTAGG
BbeI 113 GGCGCC GGCGC^C
BbrPI 7572 CACGTG CAC^GTG
BcgI 512 GCAGAGTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 617 TGATCA T^GATCA
BglII 3528 AGATCT A^GATCT
BlnI 3147 CCTAGG C^CTAGG
BsaBI 4104 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 113 GGCGCC G[GA]^CG[CT]C
BsiEI 3932 CGATCG CG[GA][CT]^CG
BsmBI 639 GAGACG CGTCTC(1/5)
BsrBI 53 GAGCGG CCGCTC(-3/-3)
BsrFI 684 ACCGGT [GA]^CCGG[CT]
BstAPI 3620 GCAGTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4630 TTCGAA TT^CGAA
Cfr10I 684 ACCGGT [GA]^CCGG[CT]
DrdI 7551 GACATCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 26 AGCGCT AGC^GCT
EcoRI 602 GAATTC G^AATTC
EcoRV 4189 GATATC GAT^ATC
EcoT22I 4440 ATGCAT ATGCA^T
FbaI 617 TGATCA T^GATCA
Hin1I 113 GGCGCC G[GA]^CG[CT]C
KasI 113 GGCGCC G^GCGCC
MamI 4104 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 113 GGCGCC GG^CGCC
NcoI 502 CCATGG C^CATGG
NsiI 4440 ATGCAT ATGCA^T
NspV 4630 TTCGAA TT^CGAA
PinAI 684 ACCGGT A^CCGGT
PmaCI 7572 CACGTG CAC^GTG
PmlI 7572 CACGTG CAC^GTG
PshAI 7577 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3932 CGATCG CGAT^CG
SfoI 113 GGCGCC GGC^GCC
SfuI 4630 TTCGAA TT^CGAA
SnaBI 6130 TACGTA TAC^GTA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1832 GTCTAC GT^[AC][GT]AC
3919 GTCTAC
AccII 151 CGCG CG^CG
1757 CGCG
AflII 5718 CTTAAG C^TTAAG
7631 CTTAAG
AflIII 3315 ACATGT A^C[GA][CT]GT
3463 ACATGT
Alw44I 5269 GTGCAC G^TGCAC
6678 GTGCAC
AocI 6411 CCTGAGG CC^T[ACGT]AGG
6842 CCTCAGG
ApaLI 5269 GTGCAC G^TGCAC
6678 GTGCAC
Asp700I 3801 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6954 GAAATAATTC
BbvCI 2654 CCTCAGC CCTCAGC(-5/-2)
7099 GCTGAGG
BfrI 5718 CTTAAG C^TTAAG
7631 CTTAAG
BsaWI 684 ACCGGT [AT]^CCGG[AT]
4421 ACCGGA
BsmI 2048 GAATGC GAATGC(1/-1)
718 GCATTC
Bsp1407I 4034 TGTACA T^GTACA
7289 TGTACA
BspLU11I 3315 ACATGT A^CATGT
3463 ACATGT
BsrGI 4034 TGTACA T^GTACA
7289 TGTACA
BssSI 1449 CTCGTG CACGAG(-5/-1)
7001 CTCGTG
BstUI 151 CGCG CG^CG
1757 CGCG
Bsu36I 6411 CCTGAGG CC^T[ACGT]AGG
6842 CCTCAGG
DsaI 502 CCATGG C^C[GA][CT]GG
7399 CCGTGG
Eco81I 6411 CCTGAGG CC^T[ACGT]AGG
6842 CCTCAGG
FauI 7626 CCCGC CCCGC(4/6)
1758 GCGGG
HaeII 26 AGCGCT [GA]GCGC^[CT]
113 GGCGCC
MspA1I 65 CAGCTG C[AC]G^C[GT]G
4311 CAGCTG
MvnI 151 CGCG CG^CG
1757 CGCG
NdeI 3511 CATATG CA^TATG
3897 CATATG
PvuII 65 CAGCTG CAG^CTG
4311 CAGCTG
SapI 1637 GAAGAGC GCTCTTC(1/4)
5191 GCTCTTC
SmaI 83 CCCGGG CCC^GGG
2434 CCCGGG
SspBI 4034 TGTACA T^GTACA
7289 TGTACA
XbaI 1777 TCTAGA T^CTAGA
4880 TCTAGA
XmaCI 83 CCCGGG C^CCGGG
2434 CCCGGG
XmaI 83 CCCGGG C^CCGGG
2434 CCCGGG
XmnI 3801 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6954 GAAATAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspI
MunI MvaI MwoI NciI NdeII
NheI NlaIII NlaIV NspI PflMI
PleI PpuMI PshBI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII