##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AatII AclI AflII AgeI AscI
Asp700I BciVI BclI BfrI BsiWI
BspHI BspLU11I BssHII Bst1107I BstZ17I
DraI DrdI EcoRI EcoRV EcoT22I
FbaI HindIII HpaI MluI NheI
NotI NruI NsiI PacI PinAI
PmeI PshAI Psp1406I PvuI RcaI
SacI SgrAI SnaBI SpeI SwaI
XmnI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 7076 GAATTT [GA]^AATT[CT]
Aor51HI 6028 AGCGCT AGC^GCT
ApoI 7076 GAATTT [GA]^AATT[CT]
AseI 7062 ATTAAT AT^TAAT
AsnI 7062 ATTAAT AT^TAAT
BamHI 1577 GGATCC G^GATCC
BglII 4550 AGATCT A^GATCT
BspDI 1924 ATCGAT AT^CGAT
BstBI 4342 TTCGAA TT^CGAA
ClaI 1924 ATCGAT AT^CGAT
CpoI 1133 CGGTCCG CG^G[AT]CCG
Eco47III 6028 AGCGCT AGC^GCT
FseI 6576 GGCCGGCC GGCCGG^CC
MfeI 2546 CAATTG C^AATTG
MunI 2546 CAATTG C^AATTG
NspV 4342 TTCGAA TT^CGAA
PshBI 7062 ATTAAT AT^TAAT
RsrII 1133 CGGTCCG CG^G[AT]CCG
SalI 6745 GTCGAC G^TCGAC
SbfI 4785 CCTGCAGG CCTGCA^GG
ScaI 2489 AGTACT AGT^ACT
SfiI 3441 GGCCCCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 4342 TTCGAA TT^CGAA
Sse8387I 4785 CCTGCAGG CCTGCA^GG
SspI 7065 AATATT AAT^ATT
XbaI 1065 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 6745 GTCGAC GT^[AC][GT]AC
6814 GTCTAC
AccIII 1136 TCCGGA T^CCGGA
4298 TCCGGA
Alw44I 109 GTGCAC G^TGCAC
3142 GTGCAC
ApaLI 109 GTGCAC G^TGCAC
3142 GTGCAC
AviII 5108 TGCGCA TGC^GCA
5288 TGCGCA
AvrII 6099 CCTAGG C^CTAGG
7360 CCTAGG
BbeI 3433 GGCGCC GGCGC^C
6919 GGCGCC
BcgI 3942 CGAGGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
684 GCACCAGGTTCG
BlnI 6099 CCTAGG C^CTAGG
7360 CCTAGG
BsaBI 1713 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4881 GATCAAGATC
BseAI 1136 TCCGGA T^CCGGA
4298 TCCGGA
Bsp1407I 707 TGTACA T^GTACA
4925 TGTACA
BspEI 1136 TCCGGA T^CCGGA
4298 TCCGGA
BsrGI 707 TGTACA T^GTACA
4925 TGTACA
EagI 449 CGGCCG C^GGCCG
2174 CGGCCG
EclXI 449 CGGCCG C^GGCCG
2174 CGGCCG
Eco52I 449 CGGCCG C^GGCCG
2174 CGGCCG
FspI 5108 TGCGCA TGC^GCA
5288 TGCGCA
HincII 6745 GTCGAC GT[CT]^[GA]AC
6796 GTCAAC
HindII 6745 GTCGAC GT[CT]^[GA]AC
6796 GTCAAC
KasI 3433 GGCGCC G^GCGCC
6919 GGCGCC
KspI 2139 CCGCGG CCGC^GG
6039 CCGCGG
MamI 1713 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4881 GATCAAGATC
MroI 1136 TCCGGA T^CCGGA
4298 TCCGGA
MseI 7063 TTAA T^TAA
8044 TTAA
NarI 3433 GGCGCC GG^CGCC
6919 GGCGCC
NdeI 3299 CATATG CA^TATG
3716 CATATG
PaeR7I 1275 CTCGAG C^TCGAG
2980 CTCGAG
SacII 2139 CCGCGG CCGC^GG
6039 CCGCGG
SapI 5733 GAAGAGC GCTCTTC(1/4)
7840 GAAGAGC
SfoI 3433 GGCGCC GGC^GCC
6919 GGCGCC
SphI 6176 GCATGC GCATG^C
7144 GCATGC
SspBI 707 TGTACA T^GTACA
4925 TGTACA
Tru9I 7063 TTAA T^TAA
8044 TTAA
XhoI 1275 CTCGAG C^TCGAG
2980 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 156 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI ApaI Asp718I AspEI AspHI
AspI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eam1105I EarI Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI