##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbvCI BciVI
BfrI BglI BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BssHII
Bst1107I BstBI BstXI BstZ17I ClaI
CpoI DraIII Eam1105I Eco47III EcoT22I
FseI FspI HaeII HgaI Hin1I
HindIII KasI KpnI MamI MluI
MluNI MroI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SwaI
Tth111I Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflIII 1542 ACGTGT A^C[GA][CT]GT
Alw44I 546 GTGCAC G^TGCAC
AocI 2389 CCTGAGG CC^T[ACGT]AGG
ApaLI 546 GTGCAC G^TGCAC
AvaII 1219 GGACC G^G[AT]CC
BamHI 934 GGATCC G^GATCC
BbsI 1403 GAAGAC GAAGAC(2/6)
BcgI 1236 CGAGCAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 919 TGATCA T^GATCA
BglII 994 AGATCT A^GATCT
BpuAI 1403 GAAGAC GAAGAC(2/6)
BsaI 44 GGTCTC GGTCTC(1/5)
BsgI 1640 CTGCAC GTGCAG(16/14)
BsrBI 1578 GAGCGG CCGCTC(-3/-3)
BsrDI 1585 CATTGC GCAATG(2/0)
BstAPI 1624 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2389 CCTGAGG CC^T[ACGT]AGG
CfoI 862 GCGC GCG^C
DraI 2076 TTTAAA TTT^AAA
DrdI 429 GACGTTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 183 CGGCCG C^GGCCG
EclXI 183 CGGCCG C^GGCCG
Eco52I 183 CGGCCG C^GGCCG
Eco81I 2389 CCTGAGG CC^T[ACGT]AGG
FbaI 919 TGATCA T^GATCA
HhaI 862 GCGC GCG^C
HinP1I 862 GCGC G^CGC
HpaI 1894 GTTAAC GTT^AAC
KspI 125 CCGCGG CCGC^GG
MfeI 2194 CAATTG C^AATTG
MunI 2194 CAATTG C^AATTG
PaeR7I 968 CTCGAG C^TCGAG
PpuMI 1218 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 896 CAGCTG CAG^CTG
SacII 125 CCGCGG CCGC^GG
SbfI 1408 CCTGCAGG CCTGCA^GG
SexAI 1359 ACCAGGT A^CC[AT]GGT
SmaI 49 CCCGGG CCC^GGG
Sse8387I 1408 CCTGCAGG CCTGCA^GG
StuI 681 AGGCCT AGG^CCT
XcmI 2308 CCAAAACCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 968 CTCGAG C^TCGAG
XmaCI 49 CCCGGG C^CCGGG
XmaI 49 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 126 CGCG CG^CG
861 CGCG
AspHI 546 GTGCAC G[AT]GC[AT]^C
1082 GAGCAC
BanI 53 GGCACC G^G[CT][GA]CC
2303 GGCACC
BanII 92 GAGCCC G[GA]GC[CT]^C
152 GGGCTC
BlpI 154 GCTCAGC GC^T[ACGT]AGC
2266 GCTAAGC
Bpu1102I 154 GCTCAGC GC^T[ACGT]AGC
2266 GCTAAGC
BsiEI 122 CGACCG CG[GA][CT]^CG
183 CGGCCG
BsiHKAI 546 GTGCAC G[AT]GC[AT]^C
1082 GAGCAC
BsmI 868 GAATGC GAATGC(1/-1)
2412 GAATGC
Bsp1407I 1929 TGTACA T^GTACA
2440 TGTACA
BsrFI 41 GCCGGT [GA]^CCGG[CT]
1368 ACCGGC
BsrGI 1929 TGTACA T^GTACA
2440 TGTACA
BssSI 375 CTCGTG CACGAG(-5/-1)
845 CTCGTG
BstEII 467 GGTGACC G^GT[ACGT]ACC
855 GGTTACC
BstPI 467 GGTGACC G^GT[ACGT]ACC
855 GGTTACC
BstUI 126 CGCG CG^CG
861 CGCG
CelII 154 GCTCAGC GC^T[ACGT]AGC
2266 GCTAAGC
Cfr10I 41 GCCGGT [GA]^CCGG[CT]
1368 ACCGGC
EcoNI 6 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1668 CCTCTGCGAGG
EcoO65I 467 GGTGACC G^GT[ACGT]ACC
855 GGTTACC
EcoRI 1488 GAATTC G^AATTC
2245 GAATTC
EcoRV 1013 GATATC GAT^ATC
1142 GATATC
FauI 527 CCCGC CCCGC(4/6)
20 GCGGG
MspA1I 125 CCGCGG C[AC]G^C[GT]G
896 CAGCTG
MvnI 126 CGCG CG^CG
861 CGCG
NcoI 87 CCATGG C^CATGG
1255 CCATGG
NspI 1764 GCATGC [GA]CATG^[CT]
2276 GCATGC
PstI 1409 CTGCAG CTGCA^G
1622 CTGCAG
SapI 1824 GAAGAGC GCTCTTC(1/4)
911 GCTCTTC
SmlI 968 CTCGAG C^T[CT][GA]AG
2090 CTTGAG
SphI 1764 GCATGC GCATG^C
2276 GCATGC
SspBI 1929 TGTACA T^GTACA
2440 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaI BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII