################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BbvCI BciVI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstBI BstXI BstZ17I ClaI CpoI DraIII Eam1105I Eco47III EcoT22I FseI FspI HaeII HgaI Hin1I HindIII KasI KpnI MamI MluI MluNI MroI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SwaI Tth111I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AflIII 1542 ACGTGT A^C[GA][CT]GT Alw44I 546 GTGCAC G^TGCAC AocI 2389 CCTGAGG CC^T[ACGT]AGG ApaLI 546 GTGCAC G^TGCAC AvaII 1219 GGACC G^G[AT]CC BamHI 934 GGATCC G^GATCC BbsI 1403 GAAGAC GAAGAC(2/6) BcgI 1236 CGAGCAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 919 TGATCA T^GATCA BglII 994 AGATCT A^GATCT BpuAI 1403 GAAGAC GAAGAC(2/6) BsaI 44 GGTCTC GGTCTC(1/5) BsgI 1640 CTGCAC GTGCAG(16/14) BsrBI 1578 GAGCGG CCGCTC(-3/-3) BsrDI 1585 CATTGC GCAATG(2/0) BstAPI 1624 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 2389 CCTGAGG CC^T[ACGT]AGG CfoI 862 GCGC GCG^C DraI 2076 TTTAAA TTT^AAA DrdI 429 GACGTTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 183 CGGCCG C^GGCCG EclXI 183 CGGCCG C^GGCCG Eco52I 183 CGGCCG C^GGCCG Eco81I 2389 CCTGAGG CC^T[ACGT]AGG FbaI 919 TGATCA T^GATCA HhaI 862 GCGC GCG^C HinP1I 862 GCGC G^CGC HpaI 1894 GTTAAC GTT^AAC KspI 125 CCGCGG CCGC^GG MfeI 2194 CAATTG C^AATTG MunI 2194 CAATTG C^AATTG PaeR7I 968 CTCGAG C^TCGAG PpuMI 1218 GGGACCC [GA]G^G[AT]CC[CT] PvuII 896 CAGCTG CAG^CTG SacII 125 CCGCGG CCGC^GG SbfI 1408 CCTGCAGG CCTGCA^GG SexAI 1359 ACCAGGT A^CC[AT]GGT SmaI 49 CCCGGG CCC^GGG Sse8387I 1408 CCTGCAGG CCTGCA^GG StuI 681 AGGCCT AGG^CCT XcmI 2308 CCAAAACCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 968 CTCGAG C^TCGAG XmaCI 49 CCCGGG C^CCGGG XmaI 49 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 126 CGCG CG^CG 861 CGCG AspHI 546 GTGCAC G[AT]GC[AT]^C 1082 GAGCAC BanI 53 GGCACC G^G[CT][GA]CC 2303 GGCACC BanII 92 GAGCCC G[GA]GC[CT]^C 152 GGGCTC BlpI 154 GCTCAGC GC^T[ACGT]AGC 2266 GCTAAGC Bpu1102I 154 GCTCAGC GC^T[ACGT]AGC 2266 GCTAAGC BsiEI 122 CGACCG CG[GA][CT]^CG 183 CGGCCG BsiHKAI 546 GTGCAC G[AT]GC[AT]^C 1082 GAGCAC BsmI 868 GAATGC GAATGC(1/-1) 2412 GAATGC Bsp1407I 1929 TGTACA T^GTACA 2440 TGTACA BsrFI 41 GCCGGT [GA]^CCGG[CT] 1368 ACCGGC BsrGI 1929 TGTACA T^GTACA 2440 TGTACA BssSI 375 CTCGTG CACGAG(-5/-1) 845 CTCGTG BstEII 467 GGTGACC G^GT[ACGT]ACC 855 GGTTACC BstPI 467 GGTGACC G^GT[ACGT]ACC 855 GGTTACC BstUI 126 CGCG CG^CG 861 CGCG CelII 154 GCTCAGC GC^T[ACGT]AGC 2266 GCTAAGC Cfr10I 41 GCCGGT [GA]^CCGG[CT] 1368 ACCGGC EcoNI 6 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1668 CCTCTGCGAGG EcoO65I 467 GGTGACC G^GT[ACGT]ACC 855 GGTTACC EcoRI 1488 GAATTC G^AATTC 2245 GAATTC EcoRV 1013 GATATC GAT^ATC 1142 GATATC FauI 527 CCCGC CCCGC(4/6) 20 GCGGG MspA1I 125 CCGCGG C[AC]G^C[GT]G 896 CAGCTG MvnI 126 CGCG CG^CG 861 CGCG NcoI 87 CCATGG C^CATGG 1255 CCATGG NspI 1764 GCATGC [GA]CATG^[CT] 2276 GCATGC PstI 1409 CTGCAG CTGCA^G 1622 CTGCAG SapI 1824 GAAGAGC GCTCTTC(1/4) 911 GCTCTTC SmlI 968 CTCGAG C^T[CT][GA]AG 2090 CTTGAG SphI 1764 GCATGC GCATG^C 2276 GCATGC SspBI 1929 TGTACA T^GTACA 2440 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaI BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII