##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Aor51HI AscI Asp718I AspEI AviII
BbrPI BcgI BfrI BsaAI BsaWI
BseAI BsiWI BspEI BssHII Bst1107I
BstEII BstPI BstZ17I Eam1105I Eco47III
EcoO65I EcoT22I FseI FspI KpnI
MluI MroI NheI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PvuI SacI SbfI
ScaI SfiI SgrAI SnaBI SpeI
Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 1327 GTCGAC GT^[AC][GT]AC
Alw44I 7677 GTGCAC G^TGCAC
ApaI 42 GGGCCC GGGCC^C
ApaLI 7677 GTGCAC G^TGCAC
AseI 6986 ATTAAT AT^TAAT
AsnI 6986 ATTAAT AT^TAAT
AspI 3895 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1332 CCTAGG C^CTAGG
BamHI 4947 GGATCC G^GATCC
BbeI 4719 GGCGCC GGCGC^C
BciVI 6242 GTATCC GTATCC(6/5)
BclI 5545 TGATCA T^GATCA
BglI 6045 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 7746 AGATCT A^GATCT
BlnI 1332 CCTAGG C^CTAGG
BlpI 5772 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5772 GCTCAGC GC^T[ACGT]AGC
BsaBI 2700 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3 CGGCCG CG[GA][CT]^CG
BsmBI 3066 GAGACG CGTCTC(1/5)
BspLU11I 5890 ACATGT A^CATGT
BstBI 1465 TTCGAA TT^CGAA
CelII 5772 GCTCAGC GC^T[ACGT]AGC
CpoI 30 CGGTCCG CG^G[AT]CCG
DrdI 3327 GACAGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3 CGGCCG C^GGCCG
EclXI 3 CGGCCG C^GGCCG
Eco52I 3 CGGCCG C^GGCCG
EcoNI 4052 CCTACCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3800 GAATTC G^AATTC
EcoRV 1857 GATATC GAT^ATC
FbaI 5545 TGATCA T^GATCA
HindIII 2673 AAGCTT A^AGCTT
KasI 4719 GGCGCC G^GCGCC
KspI 27 CCGCGG CCGC^GG
MamI 2700 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 5619 GCCGGC GCC^GGC
NarI 4719 GGCGCC GG^CGCC
NdeI 283 CATATG CA^TATG
NgoMIV 5619 GCCGGC G^CCGGC
NspV 1465 TTCGAA TT^CGAA
PflFI 3895 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2726 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6986 ATTAAT AT^TAAT
RsrII 30 CGGTCCG CG^G[AT]CCG
SacII 27 CCGCGG CCGC^GG
SalI 1327 GTCGAC G^TCGAC
SfoI 4719 GGCGCC GGC^GCC
SfuI 1465 TTCGAA TT^CGAA
SphI 3954 GCATGC GCATG^C
Tth111I 3895 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 1405 GACGTC GACGT^C
3068 GACGTC
AccII 28 CGCG CG^CG
6063 CGCG
AclI 763 AACGTT AA^CGTT
3028 AACGTT
AspHI 5069 GAGCAC G[AT]GC[AT]^C
7677 GTGCAC
BanI 4719 GGCGCC G^G[CT][GA]CC
5616 GGTGCC
BbvCI 5451 CCTCAGC CCTCAGC(-5/-2)
2778 GCTGAGG
BsgI 3275 GTGCAG GTGCAG(16/14)
4680 GTGCAG
BsiHKAI 5069 GAGCAC G[AT]GC[AT]^C
7677 GTGCAC
BspDI 2385 ATCGAT AT^CGAT
3597 ATCGAT
BspMI 2374 GCAGGT ACCTGC(4/8)
5027 GCAGGT
BsrBI 5969 CCGCTC CCGCTC(-3/-3)
1551 GAGCGG
BsrDI 496 GCAATG GCAATG(2/0)
1585 GCAATG
BsrFI 998 GCCGGT [GA]^CCGG[CT]
5619 GCCGGC
BssSI 4406 CACGAG CACGAG(-5/-1)
7811 CTCGTG
BstUI 28 CGCG CG^CG
6063 CGCG
Cfr10I 998 GCCGGT [GA]^CCGG[CT]
5619 GCCGGC
ClaI 2385 ATCGAT AT^CGAT
3597 ATCGAT
DraIII 7316 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
7586 CACGGTGTG
HaeII 1384 GGCGCT [GA]GCGC^[CT]
4719 GGCGCC
HpaI 294 GTTAAC GTT^AAC
6840 GTTAAC
MfeI 228 CAATTG C^AATTG
546 CAATTG
MunI 228 CAATTG C^AATTG
546 CAATTG
MvnI 28 CGCG CG^CG
6063 CGCG
PpuMI 1448 AGGTCCC [GA]G^G[AT]CC[CT]
4789 AGGACCC
Psp1406I 763 AACGTT AA^CGTT
3028 AACGTT
SapI 2796 GAAGAGC GCTCTTC(1/4)
5140 GCTCTTC
SmaI 38 CCCGGG CCC^GGG
46 CCCGGG
XmaCI 38 CCCGGG C^CCGGG
46 CCCGGG
XmaI 38 CCCGGG C^CCGGG
46 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI