##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
AatII Acc65I AflII AgeI Alw44I
Aor51HI ApaLI AscI Asp718I AvrII
BcgI BciVI BfrI BglI BlnI
BsiWI BssHII Bst1107I BstZ17I CpoI
DraIII Eco47III FseI KpnI KspI
MluI NheI NotI NruI PacI
PinAI PmeI PvuI RsrII SacII
SalI SbfI SfiI SgrAI SmaI
SnaBI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 3429 TCCGGA T^CCGGA
AclI 5229 AACGTT AA^CGTT
AcyI 4970 GGCGCC G[GA]^CG[CT]C
AhdI 4096 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 4345 GGGCCC GGGCC^C
AspEI 4096 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4848 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 8256 TGCGCA TGC^GCA
BbeI 4970 GGCGCC GGCGC^C
BbrPI 3896 CACGTG CAC^GTG
BsaHI 4970 GGCGCC G[GA]^CG[CT]C
BseAI 3429 TCCGGA T^CCGGA
BsiEI 5607 CGGCCG CG[GA][CT]^CG
BspDI 1865 ATCGAT AT^CGAT
BspEI 3429 TCCGGA T^CCGGA
BsrFI 7020 GCCGGC [GA]^CCGG[CT]
BssSI 1373 CACGAG CACGAG(-5/-1)
BstBI 4722 TTCGAA TT^CGAA
Cfr10I 7020 GCCGGC [GA]^CCGG[CT]
ClaI 1865 ATCGAT AT^CGAT
DrdI 7139 GACAGACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 5607 CGGCCG C^GGCCG
Eam1105I 4096 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 5607 CGGCCG C^GGCCG
Eco52I 5607 CGGCCG C^GGCCG
EcoRV 4198 GATATC GAT^ATC
FspI 8256 TGCGCA TGC^GCA
HaeII 4970 GGCGCC [GA]GCGC^[CT]
Hin1I 4970 GGCGCC G[GA]^CG[CT]C
HindIII 5626 AAGCTT A^AGCTT
HpaI 5226 GTTAAC GTT^AAC
KasI 4970 GGCGCC G^GCGCC
MroI 3429 TCCGGA T^CCGGA
NaeI 7020 GCCGGC GCC^GGC
NarI 4970 GGCGCC GG^CGCC
NdeI 7571 CATATG CA^TATG
NgoMIV 7020 GCCGGC G^CCGGC
NspV 4722 TTCGAA TT^CGAA
PflFI 4848 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 6957 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3896 CACGTG CAC^GTG
PmlI 3896 CACGTG CAC^GTG
Psp1406I 5229 AACGTT AA^CGTT
SfoI 4970 GGCGCC GGC^GCC
SfuI 4722 TTCGAA TT^CGAA
SpeI 2584 ACTAGT A^CTAGT
Tth111I 4848 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 6957 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1246 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 1340 CGCG CG^CG
5789 CGCG
AocI 1455 CCTAAGG CC^T[ACGT]AGG
7822 CCTGAGG
AseI 1816 ATTAAT AT^TAAT
6163 ATTAAT
AsnI 1816 ATTAAT AT^TAAT
6163 ATTAAT
Asp700I 877 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5271 GAAATGTTTC
AvaI 4781 CTCGAG C^[CT]CG[GA]G
7436 CTCGAG
BbvCI 4941 CCTCAGC CCTCAGC(-5/-2)
7324 CCTCAGC
BsaWI 3429 TCCGGA [AT]^CCGG[AT]
7709 ACCGGA
BsmI 4496 GAATGC GAATGC(1/-1)
6046 GAATGC
BsoBI 4781 CTCGAG C^[CT]CG[GA]G
7436 CTCGAG
BsrBI 1 CCGCTC CCGCTC(-3/-3)
6508 GAGCGG
BstAPI 4905 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6657 GCAACAGATGC
BstUI 1340 CGCG CG^CG
5789 CGCG
Bsu36I 1455 CCTAAGG CC^T[ACGT]AGG
7822 CCTGAGG
DsaI 3032 CCATGG C^C[GA][CT]GG
3245 CCATGG
Eco81I 1455 CCTAAGG CC^T[ACGT]AGG
7822 CCTGAGG
EcoT22I 2148 ATGCAT ATGCA^T
3059 ATGCAT
HgaI 1338 GACGC GACGC(5/10)
6112 GACGC
HincII 5226 GTTAAC GT[CT]^[GA]AC
8079 GTCAAC
HindII 5226 GTTAAC GT[CT]^[GA]AC
8079 GTCAAC
MfeI 5292 CAATTG C^AATTG
7196 CAATTG
MunI 5292 CAATTG C^AATTG
7196 CAATTG
MvnI 1340 CGCG CG^CG
5789 CGCG
NcoI 3032 CCATGG C^CATGG
3245 CCATGG
NsiI 2148 ATGCAT ATGCA^T
3059 ATGCAT
PaeR7I 4781 CTCGAG C^TCGAG
7436 CTCGAG
PpuMI 4373 GGGACCC [GA]G^G[AT]CC[CT]
7826 AGGACCT
PshAI 1628 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5736 GACTGCTGTC
PshBI 1816 ATTAAT AT^TAAT
6163 ATTAAT
SexAI 5441 ACCAGGT A^CC[AT]GGT
6747 ACCAGGT
StuI 6764 AGGCCT AGG^CCT
7592 AGGCCT
XhoI 4781 CTCGAG C^TCGAG
7436 CTCGAG
XmnI 877 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5271 GAAATGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BamHI BanI BanII
BbsI BbvI BclI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I Bsp1407I
BspHI BspLU11I BspMI BsrDI BsrGI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII