##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AflII AgeI AseI AsnI Asp700I
AspI AviII AvrII BclI BfrI
BlnI BsaBI BsiWI BspDI Bst1107I
BstZ17I ClaI EcoRV EcoT22I FbaI
FseI FspI HindIII MamI MfeI
MluI MunI NheI NruI NsiI
PacI PflFI PinAI PmeI PshBI
PvuI SalI SexAI SgrAI SnaBI
SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 3698 GGTACC G^GTACC
AccIII 419 TCCGGA T^CCGGA
AclI 6754 AACGTT AA^CGTT
AhdI 5483 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1561 GTGCAC G^TGCAC
Aor51HI 994 AGCGCT AGC^GCT
ApaLI 1561 GTGCAC G^TGCAC
AscI 611 GGCGCGCC GG^CGCGCC
Asp718I 3698 GGTACC G^GTACC
AspEI 5483 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 4528 GTATCC GTATCC(6/5)
BglII 6258 AGATCT A^GATCT
BsaI 2313 GGTCTC GGTCTC(1/5)
BseAI 419 TCCGGA T^CCGGA
BsmBI 1029 CGTCTC CGTCTC(1/5)
BspEI 419 TCCGGA T^CCGGA
BstBI 6784 TTCGAA TT^CGAA
BstEII 1634 GGTGACC G^GT[ACGT]ACC
BstPI 1634 GGTGACC G^GT[ACGT]ACC
CpoI 430 CGGTCCG CG^G[AT]CCG
Eam1105I 5483 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 994 AGCGCT AGC^GCT
EcoO65I 1634 GGTGACC G^GT[ACGT]ACC
EcoRI 1389 GAATTC G^AATTC
HincII 2346 GTTAAC GT[CT]^[GA]AC
HindII 2346 GTTAAC GT[CT]^[GA]AC
HpaI 2346 GTTAAC GTT^AAC
KpnI 3698 GGTACC GGTAC^C
MroI 419 TCCGGA T^CCGGA
NdeI 1498 CATATG CA^TATG
NotI 2511 GCGGCCGC GC^GGCCGC
NspV 6784 TTCGAA TT^CGAA
PaeR7I 487 CTCGAG C^TCGAG
PshAI 156 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6754 AACGTT AA^CGTT
RsrII 430 CGGTCCG CG^G[AT]CCG
SapI 859 GAAGAGC GCTCTTC(1/4)
SbfI 1400 CCTGCAGG CCTGCA^GG
SfuI 6784 TTCGAA TT^CGAA
SpeI 3113 ACTAGT A^CTAGT
Sse8387I 1400 CCTGCAGG CCTGCA^GG
XbaI 5154 TCTAGA T^CTAGA
XcmI 2956 CCATTTGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 487 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AatII 1027 GACGTC GACGT^C
3002 GACGTC
AccI 6876 GTCTAC GT^[AC][GT]AC
6920 GTCTAC
ApaI 539 GGGCCC GGGCC^C
4013 GGGCCC
BbrPI 2784 CACGTG CAC^GTG
6335 CACGTG
BbsI 386 GAAGAC GAAGAC(2/6)
675 GTCTTC
BpuAI 386 GAAGAC GAAGAC(2/6)
675 GTCTTC
BspHI 3953 TCATGA T^CATGA
4692 TCATGA
BsrDI 5588 CATTGC GCAATG(2/0)
6318 GCAATG
BssSI 2777 CACGAG CACGAG(-5/-1)
291 CTCGTG
BstXI 1070 CCACTAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2351 CCAAGCGGCTGG
EcoNI 5511 CCTAATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6214 CCTGCGCGAGG
PmaCI 2784 CACGTG CAC^GTG
6335 CACGTG
PmlI 2784 CACGTG CAC^GTG
6335 CACGTG
RcaI 3953 TCATGA T^CATGA
4692 TCATGA
SfiI 427 GGCCGGTCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1080 GGCCCTGAGGGCC
SspI 3390 AATATT AAT^ATT
4217 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 155 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BamHI BanI BanII BbeI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspLU11I BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DrdI
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SacII
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SfoI SmaI SmlI SphI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI