##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
ApaI ApaLI AscI AspEI AviII
BbeI BbrPI BcgI BciVI BsiWI
BsmBI BspDI BssHII BstBI ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoRV FseI FspI KasI MluI
NaeI NarI NgoMIV NotI NruI
NspV PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SwaI XcmI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 1886 GGTACC G^GTACC
AccII 5524 CGCG CG^CG
AccIII 2164 TCCGGA T^CCGGA
AcyI 6745 GACGCC G[GA]^CG[CT]C
AflII 8097 CTTAAG C^TTAAG
AlwNI 6143 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1886 GGTACC G^GTACC
AvrII 2525 CCTAGG C^CTAGG
BamHI 6033 GGATCC G^GATCC
BclI 1445 TGATCA T^GATCA
BfrI 8097 CTTAAG C^TTAAG
BglI 6820 GCCGAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2525 CCTAGG C^CTAGG
BsaAI 591 TACGTG [CT]AC^GT[GA]
BsaHI 6745 GACGCC G[GA]^CG[CT]C
BsaWI 2164 TCCGGA [AT]^CCGG[AT]
BseAI 2164 TCCGGA T^CCGGA
BsiEI 5997 CGACCG CG[GA][CT]^CG
BspEI 2164 TCCGGA T^CCGGA
BsrFI 6748 GCCGGT [GA]^CCGG[CT]
BssSI 228 CACGAG CACGAG(-5/-1)
Bst1107I 6246 GTATAC GTA^TAC
BstAPI 5455 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 5926 GGTTACC G^GT[ACGT]ACC
BstPI 5926 GGTTACC G^GT[ACGT]ACC
BstUI 5524 CGCG CG^CG
BstZ17I 6246 GTATAC GTA^TAC
Cfr10I 6748 GCCGGT [GA]^CCGG[CT]
DrdI 5368 GACTGTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 6444 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5926 GGTTACC G^GT[ACGT]ACC
EcoRI 2820 GAATTC G^AATTC
FbaI 1445 TGATCA T^GATCA
Hin1I 6745 GACGCC G[GA]^CG[CT]C
HpaI 1607 GTTAAC GTT^AAC
KpnI 1886 GGTACC GGTAC^C
KspI 5523 CCGCGG CCGC^GG
MfeI 5831 CAATTG C^AATTG
MroI 2164 TCCGGA T^CCGGA
MunI 5831 CAATTG C^AATTG
MvnI 5524 CGCG CG^CG
NheI 59 GCTAGC G^CTAGC
PaeR7I 3346 CTCGAG C^TCGAG
PshAI 6745 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 5523 CCGCGG CCGC^GG
ScaI 2059 AGTACT AGT^ACT
SexAI 5058 ACCTGGT A^CC[AT]GGT
SpeI 4040 ACTAGT A^CTAGT
XhoI 3346 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AocI 1081 CCTGAGG CC^T[ACGT]AGG
2424 CCTCAGG
Aor51HI 2688 AGCGCT AGC^GCT
6984 AGCGCT
BlpI 385 GCTGAGC GC^T[ACGT]AGC
4874 GCTGAGC
Bpu1102I 385 GCTGAGC GC^T[ACGT]AGC
4874 GCTGAGC
BspHI 2762 TCATGA T^CATGA
3948 TCATGA
Bsu36I 1081 CCTGAGG CC^T[ACGT]AGG
2424 CCTCAGG
CelII 385 GCTGAGC GC^T[ACGT]AGC
4874 GCTGAGC
DraIII 5230 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
5323 CACATTGTG
Eco47III 2688 AGCGCT AGC^GCT
6984 AGCGCT
Eco81I 1081 CCTGAGG CC^T[ACGT]AGG
2424 CCTCAGG
EcoT22I 2020 ATGCAT ATGCA^T
6209 ATGCAT
HaeII 2688 AGCGCT [GA]GCGC^[CT]
6984 AGCGCT
NdeI 833 CATATG CA^TATG
4062 CATATG
NsiI 2020 ATGCAT ATGCA^T
6209 ATGCAT
PacI 5737 TTAATTAA TTAAT^TAA
5869 TTAATTAA
PstI 3241 CTGCAG CTGCA^G
8176 CTGCAG
PvuII 6548 CAGCTG CAG^CTG
7702 CAGCTG
RcaI 2762 TCATGA T^CATGA
3948 TCATGA
SapI 7175 GAAGAGC GCTCTTC(1/4)
7265 GAAGAGC
SmaI 377 CCCGGG CCC^GGG
1222 CCCGGG
SphI 963 GCATGC GCATG^C
5517 GCATGC
StuI 7861 AGGCCT AGG^CCT
7953 AGGCCT
XmaCI 377 CCCGGG C^CCGGG
1222 CCCGGG
XmaI 377 CCCGGG C^CCGGG
1222 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
Asp700I AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PshBI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XhoII XmnI