##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
AscI Asp718I AviII AvrII BalI
BfrI BlnI Bsp1407I BspDI BspHI
BsrGI Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DrdI FspI KpnI
MluI MluNI MscI NheI NruI
NspV PacI PinAI PmeI PvuI
RcaI RsrII SalI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 116 TCCGGA T^CCGGA
AclI 1018 AACGTT AA^CGTT
AhdI 4309 GACGGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2540 AGCGCT AGC^GCT
AseI 6986 ATTAAT AT^TAAT
AsnI 6986 ATTAAT AT^TAAT
AspEI 4309 GACGGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5512 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 4 GGCGCC GGCGC^C
BciVI 1949 GGATAC GTATCC(6/5)
BsaBI 3421 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 116 TCCGGA [AT]^CCGG[AT]
BseAI 116 TCCGGA T^CCGGA
BsiWI 5678 CGTACG C^GTACG
BsmI 1538 GCATTC GAATGC(1/-1)
BspEI 116 TCCGGA T^CCGGA
BspLU11I 5600 ACATGT A^CATGT
BssHII 10 GCGCGC G^CGCGC
Eam1105I 4309 GACGGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2540 AGCGCT AGC^GCT
EcoRI 3106 GAATTC G^AATTC
EcoRV 3001 GATATC GAT^ATC
EcoT22I 2132 ATGCAT ATGCA^T
HindIII 3274 AAGCTT A^AGCTT
KasI 4 GGCGCC G^GCGCC
KspI 3497 CCGCGG CCGC^GG
MamI 3421 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3945 CAATTG C^AATTG
MroI 116 TCCGGA T^CCGGA
MunI 3945 CAATTG C^AATTG
NarI 4 GGCGCC GG^CGCC
NotI 4462 GCGGCCGC GC^GGCCGC
NsiI 2132 ATGCAT ATGCA^T
PaeR7I 4551 CTCGAG C^TCGAG
PflFI 5512 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 6986 ATTAAT AT^TAAT
Psp1406I 1018 AACGTT AA^CGTT
SacI 5379 GAGCTC GAGCT^C
SacII 3497 CCGCGG CCGC^GG
SexAI 7569 ACCAGGT A^CC[AT]GGT
SfoI 4 GGCGCC GGC^GCC
SspI 3076 AATATT AAT^ATT
Tth111I 5512 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 4551 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 1130 GTGCAC G^TGCAC
6603 GTGCAC
ApaLI 1130 GTGCAC G^TGCAC
6603 GTGCAC
Asp700I 5015 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7138 GAAACTCTTC
BamHI 4476 GGATCC G^GATCC
5829 GGATCC
BbrPI 5494 CACGTG CAC^GTG
5891 CACGTG
BcgI 2016 GCACAGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2086 GCAGCCTCCTCG
BclI 4949 TGATCA T^GATCA
5595 TGATCA
BlpI 1774 GCTGAGC GC^T[ACGT]AGC
4664 GCTCAGC
Bpu1102I 1774 GCTGAGC GC^T[ACGT]AGC
4664 GCTCAGC
BsmBI 2255 GAGACG CGTCTC(1/5)
5510 GAGACG
BsrBI 112 CCGCTC CCGCTC(-3/-3)
1372 GAGCGG
BstEII 1051 GGTTACC G^GT[ACGT]ACC
7124 GGTCACC
BstPI 1051 GGTTACC G^GT[ACGT]ACC
7124 GGTCACC
CelII 1774 GCTGAGC GC^T[ACGT]AGC
4664 GCTCAGC
EcoNI 525 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5608 CCTCTGCCAGG
EcoO65I 1051 GGTTACC G^GT[ACGT]ACC
7124 GGTCACC
FbaI 4949 TGATCA T^GATCA
5595 TGATCA
FseI 4498 GGCCGGCC GGCCGG^CC
7184 GGCCGGCC
HpaI 3622 GTTAAC GTT^AAC
6251 GTTAAC
NdeI 4776 CATATG CA^TATG
5797 CATATG
PmaCI 5494 CACGTG CAC^GTG
5891 CACGTG
PmlI 5494 CACGTG CAC^GTG
5891 CACGTG
SapI 1191 GAAGAGC GCTCTTC(1/4)
1807 GAAGAGC
XcmI 1168 CCAGAAAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1868 CCAAGTTTGCCCTGG
XmnI 5015 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7138 GAAACTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BglII BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
SbfI ScaI ScrFI SfaNI SfcI
SmaI SmlI Sse8387I StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI