################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII AccII AclI AgeI Aor51HI AscI AvrII BcgI BlnI BlpI Bpu1102I BsiEI BsiWI Bsp1407I BspDI BsrFI BsrGI BssHII BstAPI BstBI BstUI CelII Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I FseI KspI MluI MvnI NaeI NgoMIV NheI NotI NruI NspV PacI PinAI PmeI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfuI SgrAI SmaI SpeI Sse8387I SspBI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccIII 6511 TCCGGA T^CCGGA AhdI 4522 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 5285 CCTGAGG CC^T[ACGT]AGG AspEI 4522 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 6363 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 6637 TGCGCA TGC^GCA BalI 2739 TGGCCA TGG^CCA BbeI 391 GGCGCC GGCGC^C BbvCI 312 CCTCAGC CCTCAGC(-5/-2) BclI 1020 TGATCA T^GATCA BglI 3478 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseAI 6511 TCCGGA T^CCGGA BspEI 6511 TCCGGA T^CCGGA BsrBI 997 CCGCTC CCGCTC(-3/-3) BstXI 5390 CCAAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 5285 CCTGAGG CC^T[ACGT]AGG DrdI 3573 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1580 CCATGG C^C[GA][CT]GG Eam1105I 4522 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 5285 CCTGAGG CC^T[ACGT]AGG EcoRI 7246 GAATTC G^AATTC EcoT14I 1580 CCATGG C^C[AT][AT]GG FauI 6372 CCCGC CCCGC(4/6) FbaI 1020 TGATCA T^GATCA FspI 6637 TGCGCA TGC^GCA HaeII 391 GGCGCC [GA]GCGC^[CT] KasI 391 GGCGCC G^GCGCC MfeI 7724 CAATTG C^AATTG MluNI 2739 TGGCCA TGG^CCA MroI 6511 TCCGGA T^CCGGA MscI 2739 TGGCCA TGG^CCA MunI 7724 CAATTG C^AATTG NarI 391 GGCGCC GG^CGCC NcoI 1580 CCATGG C^CATGG NdeI 1260 CATATG CA^TATG PflFI 6363 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC SexAI 5531 ACCAGGT A^CC[AT]GGT SfoI 391 GGCGCC GGC^GCC SnaBI 2509 TACGTA TAC^GTA SphI 664 GCATGC GCATG^C StyI 1580 CCATGG C^C[AT][AT]GG SwaI 5496 ATTTAAAT ATTT^AAAT Tth111I 6363 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 4887 GGTACC G^GTACC 8357 GGTACC AcyI 391 GGCGCC G[GA]^CG[CT]C 7093 GGCGTC ApaI 772 GGGCCC GGGCC^C 3607 GGGCCC AseI 1664 ATTAAT AT^TAAT 5542 ATTAAT AsnI 1664 ATTAAT AT^TAAT 5542 ATTAAT Asp700I 5599 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5796 GAAGCATTTC Asp718I 4887 GGTACC G^GTACC 8357 GGTACC BamHI 434 GGATCC G^GATCC 5162 GGATCC BbrPI 8031 CACGTG CAC^GTG 8093 CACGTG BciVI 1117 GGATAC GTATCC(6/5) 2347 GGATAC BglII 4413 AGATCT A^GATCT 7708 AGATCT BsaBI 3860 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 7138 GATTCTTATC BsaHI 391 GGCGCC G[GA]^CG[CT]C 7093 GGCGTC BsaWI 5190 ACCGGA [AT]^CCGG[AT] 6511 TCCGGA BstEII 1343 GGTCACC G^GT[ACGT]ACC 4628 GGTCACC BstPI 1343 GGTCACC G^GT[ACGT]ACC 4628 GGTCACC CfoI 392 GCGC GCG^C 6638 GCGC EcoO65I 1343 GGTCACC G^GT[ACGT]ACC 4628 GGTCACC EcoRV 1238 GATATC GAT^ATC 7793 GATATC EcoT22I 135 ATGCAT ATGCA^T 2377 ATGCAT HhaI 392 GCGC GCG^C 6638 GCGC Hin1I 391 GGCGCC G[GA]^CG[CT]C 7093 GGCGTC HinP1I 392 GCGC G^CGC 6638 GCGC KpnI 4887 GGTACC GGTAC^C 8357 GGTACC MamI 3860 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 7138 GATTCTTATC NsiI 135 ATGCAT ATGCA^T 2377 ATGCAT PaeR7I 608 CTCGAG C^TCGAG 4005 CTCGAG PflMI 2338 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 7277 CCACAGTGTGG PmaCI 8031 CACGTG CAC^GTG 8093 CACGTG PmlI 8031 CACGTG CAC^GTG 8093 CACGTG PshBI 1664 ATTAAT AT^TAAT 5542 ATTAAT Van91I 2338 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 7277 CCACAGTGTGG XbaI 3150 TCTAGA T^CTAGA 4182 TCTAGA XhoI 608 CTCGAG C^TCGAG 4005 CTCGAG XmnI 5599 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5796 GAAGCATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BfrI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspHI BspLU11I BspMI BsrDI BsrI BssKI BssSI Bst1107I BstF5I BstNI BstYI BstZ17I Cac8I Cfr13I DdeI DpnII DraI DraII DraIII EaeI EarI EcoNI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI HincII HindII HindIII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PstI PvuII RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SspI StuI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII