##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccII AclI AgeI Aor51HI
AscI AvrII BcgI BlnI BlpI
Bpu1102I BsiEI BsiWI Bsp1407I BspDI
BsrFI BsrGI BssHII BstAPI BstBI
BstUI CelII Cfr10I ClaI CpoI
EagI EclXI Eco47III Eco52I FseI
KspI MluI MvnI NaeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmeI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfuI SgrAI SmaI SpeI
Sse8387I SspBI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 6511 TCCGGA T^CCGGA
AhdI 4522 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 5285 CCTGAGG CC^T[ACGT]AGG
AspEI 4522 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 6363 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 6637 TGCGCA TGC^GCA
BalI 2739 TGGCCA TGG^CCA
BbeI 391 GGCGCC GGCGC^C
BbvCI 312 CCTCAGC CCTCAGC(-5/-2)
BclI 1020 TGATCA T^GATCA
BglI 3478 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 6511 TCCGGA T^CCGGA
BspEI 6511 TCCGGA T^CCGGA
BsrBI 997 CCGCTC CCGCTC(-3/-3)
BstXI 5390 CCAAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 5285 CCTGAGG CC^T[ACGT]AGG
DrdI 3573 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1580 CCATGG C^C[GA][CT]GG
Eam1105I 4522 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5285 CCTGAGG CC^T[ACGT]AGG
EcoRI 7246 GAATTC G^AATTC
EcoT14I 1580 CCATGG C^C[AT][AT]GG
FauI 6372 CCCGC CCCGC(4/6)
FbaI 1020 TGATCA T^GATCA
FspI 6637 TGCGCA TGC^GCA
HaeII 391 GGCGCC [GA]GCGC^[CT]
KasI 391 GGCGCC G^GCGCC
MfeI 7724 CAATTG C^AATTG
MluNI 2739 TGGCCA TGG^CCA
MroI 6511 TCCGGA T^CCGGA
MscI 2739 TGGCCA TGG^CCA
MunI 7724 CAATTG C^AATTG
NarI 391 GGCGCC GG^CGCC
NcoI 1580 CCATGG C^CATGG
NdeI 1260 CATATG CA^TATG
PflFI 6363 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 5531 ACCAGGT A^CC[AT]GGT
SfoI 391 GGCGCC GGC^GCC
SnaBI 2509 TACGTA TAC^GTA
SphI 664 GCATGC GCATG^C
StyI 1580 CCATGG C^C[AT][AT]GG
SwaI 5496 ATTTAAAT ATTT^AAAT
Tth111I 6363 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 4887 GGTACC G^GTACC
8357 GGTACC
AcyI 391 GGCGCC G[GA]^CG[CT]C
7093 GGCGTC
ApaI 772 GGGCCC GGGCC^C
3607 GGGCCC
AseI 1664 ATTAAT AT^TAAT
5542 ATTAAT
AsnI 1664 ATTAAT AT^TAAT
5542 ATTAAT
Asp700I 5599 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5796 GAAGCATTTC
Asp718I 4887 GGTACC G^GTACC
8357 GGTACC
BamHI 434 GGATCC G^GATCC
5162 GGATCC
BbrPI 8031 CACGTG CAC^GTG
8093 CACGTG
BciVI 1117 GGATAC GTATCC(6/5)
2347 GGATAC
BglII 4413 AGATCT A^GATCT
7708 AGATCT
BsaBI 3860 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7138 GATTCTTATC
BsaHI 391 GGCGCC G[GA]^CG[CT]C
7093 GGCGTC
BsaWI 5190 ACCGGA [AT]^CCGG[AT]
6511 TCCGGA
BstEII 1343 GGTCACC G^GT[ACGT]ACC
4628 GGTCACC
BstPI 1343 GGTCACC G^GT[ACGT]ACC
4628 GGTCACC
CfoI 392 GCGC GCG^C
6638 GCGC
EcoO65I 1343 GGTCACC G^GT[ACGT]ACC
4628 GGTCACC
EcoRV 1238 GATATC GAT^ATC
7793 GATATC
EcoT22I 135 ATGCAT ATGCA^T
2377 ATGCAT
HhaI 392 GCGC GCG^C
6638 GCGC
Hin1I 391 GGCGCC G[GA]^CG[CT]C
7093 GGCGTC
HinP1I 392 GCGC G^CGC
6638 GCGC
KpnI 4887 GGTACC GGTAC^C
8357 GGTACC
MamI 3860 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7138 GATTCTTATC
NsiI 135 ATGCAT ATGCA^T
2377 ATGCAT
PaeR7I 608 CTCGAG C^TCGAG
4005 CTCGAG
PflMI 2338 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7277 CCACAGTGTGG
PmaCI 8031 CACGTG CAC^GTG
8093 CACGTG
PmlI 8031 CACGTG CAC^GTG
8093 CACGTG
PshBI 1664 ATTAAT AT^TAAT
5542 ATTAAT
Van91I 2338 CCAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7277 CCACAGTGTGG
XbaI 3150 TCTAGA T^CTAGA
4182 TCTAGA
XhoI 608 CTCGAG C^TCGAG
4005 CTCGAG
XmnI 5599 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5796 GAAGCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BfrI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspLU11I BspMI BsrDI BsrI BssKI
BssSI Bst1107I BstF5I BstNI BstYI
BstZ17I Cac8I Cfr13I DdeI DpnII
DraI DraII DraIII EaeI EarI
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HgaI HincII HindII
HindIII HinfI HpaI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PshAI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StuI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII