##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BclI BlnI
BlpI Bpu1102I BsiWI BsrDI BssHII
Bst1107I BstBI BstZ17I CelII CpoI
Eam1105I FbaI FseI FspI HpaI
KpnI KspI MluI NheI NotI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SapI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 6966 TCCGGA T^CCGGA
Aor51HI 4202 AGCGCT AGC^GCT
BbeI 1217 GGCGCC GGCGC^C
BciVI 3606 GGATAC GTATCC(6/5)
BseAI 6966 TCCGGA T^CCGGA
BsmI 6008 GAATGC GAATGC(1/-1)
Bsp1407I 3473 TGTACA T^GTACA
BspDI 715 ATCGAT AT^CGAT
BspEI 6966 TCCGGA T^CCGGA
BsrGI 3473 TGTACA T^GTACA
ClaI 715 ATCGAT AT^CGAT
DrdI 5970 GACATGTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4202 AGCGCT AGC^GCT
EcoRV 718 GATATC GAT^ATC
EcoT22I 6010 ATGCAT ATGCA^T
HindIII 7756 AAGCTT A^AGCTT
KasI 1217 GGCGCC G^GCGCC
MfeI 6257 CAATTG C^AATTG
MroI 6966 TCCGGA T^CCGGA
MunI 6257 CAATTG C^AATTG
NarI 1217 GGCGCC GG^CGCC
NcoI 7410 CCATGG C^CATGG
NdeI 303 CATATG CA^TATG
NruI 7506 TCGCGA TCG^CGA
NsiI 6010 ATGCAT ATGCA^T
PaeR7I 6802 CTCGAG C^TCGAG
SalI 6678 GTCGAC G^TCGAC
SbfI 3419 CCTGCAGG CCTGCA^GG
SfoI 1217 GGCGCC GGC^GCC
Sse8387I 3419 CCTGCAGG CCTGCA^GG
SspBI 3473 TGTACA T^GTACA
XcmI 2700 CCACCGGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 6802 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 1344 GTAGAC GT^[AC][GT]AC
6678 GTCGAC
AcyI 1217 GGCGCC G[GA]^CG[CT]C
6391 GGCGTC
ApaI 1016 GGGCCC GGGCC^C
1241 GGGCCC
BbrPI 3495 CACGTG CAC^GTG
7454 CACGTG
BglI 931 GCCGCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1227 GCCACGGTGGC
BglII 3135 AGATCT A^GATCT
5492 AGATCT
BsaBI 245 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4491 GATGGGAATC
BsaHI 1217 GGCGCC G[GA]^CG[CT]C
6391 GGCGTC
BspHI 3130 TCATGA T^CATGA
7072 TCATGA
EcoRI 4302 GAATTC G^AATTC
7628 GAATTC
Hin1I 1217 GGCGCC G[GA]^CG[CT]C
6391 GGCGTC
MamI 245 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4491 GATGGGAATC
PflMI 5424 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6727 CCAGACGCTGG
PmaCI 3495 CACGTG CAC^GTG
7454 CACGTG
PmlI 3495 CACGTG CAC^GTG
7454 CACGTG
PvuII 5996 CAGCTG CAG^CTG
6412 CAGCTG
RcaI 3130 TCATGA T^CATGA
7072 TCATGA
SmaI 1013 CCCGGG CCC^GGG
5389 CCCGGG
SspI 7018 AATATT AAT^ATT
7145 AATATT
Van91I 5424 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6727 CCAGACGCTGG
XmaCI 1013 CCCGGG C^CCGGG
5389 CCCGGG
XmaI 1013 CCCGGG C^CCGGG
5389 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AspHI AspI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PleI PpuMI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoII