##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AgeI Aor51HI ApoI AscI AseI
AsnI Asp718I BamHI BbrPI BciVI
BglII BsaBI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI HpaI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 1460 GTCGAC GT^[AC][GT]AC
AflII 1744 CTTAAG C^TTAAG
AhdI 969 GACTACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 573 GTGCAC G^TGCAC
AlwNI 1443 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1104 CCTGAGG CC^T[ACGT]AGG
ApaLI 573 GTGCAC G^TGCAC
AspEI 969 GACTACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 769 TGCGCA TGC^GCA
BalI 874 TGGCCA TGG^CCA
BclI 1672 TGATCA T^GATCA
BfrI 1744 CTTAAG C^TTAAG
BsaAI 275 TACGTG [CT]AC^GT[GA]
BsaI 50 GAGACC GGTCTC(1/5)
BsmBI 105 GAGACG CGTCTC(1/5)
BsmI 2197 GCATTC GAATGC(1/-1)
BspLU11I 973 ACATGT A^CATGT
BsrDI 148 CATTGC GCAATG(2/0)
BssSI 2303 CACGAG CACGAG(-5/-1)
BstXI 2138 CCAGCTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1639 AGATCC [GA]^GATC[CT]
Bsu36I 1104 CCTGAGG CC^T[ACGT]AGG
DraIII 536 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 154 CCATGG C^C[GA][CT]GG
Eam1105I 969 GACTACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1104 CCTGAGG CC^T[ACGT]AGG
EcoNI 35 CCTCGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1672 TGATCA T^GATCA
FspI 769 TGCGCA TGC^GCA
HindIII 375 AAGCTT A^AGCTT
MflI 1639 AGATCC [GA]^GATC[CT]
MluNI 874 TGGCCA TGG^CCA
MscI 874 TGGCCA TGG^CCA
MslI 2115 CATGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 154 CCATGG C^CATGG
NspI 973 ACATGT [GA]CATG^[CT]
PaeR7I 1377 CTCGAG C^TCGAG
SacI 1292 GAGCTC GAGCT^C
SalI 1460 GTCGAC G^TCGAC
SapI 1002 GAAGAGC GCTCTTC(1/4)
SfiI 554 GGCCTTCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
XcmI 1442 CCAGAGCCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1377 CTCGAG C^TCGAG
XhoII 1639 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 91 CGCG CG^CG
353 CGCG
AfaI 404 GTAC GT^AC
584 GTAC
ApaI 1044 GGGCCC GGGCC^C
1866 GGGCCC
Asp700I 668 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1577 GAAAAAGTTC
AspHI 573 GTGCAC G[AT]GC[AT]^C
1292 GAGCTC
AspI 258 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2385 GACTCTGTC
AvrII 21 CCTAGG C^CTAGG
164 CCTAGG
BbvCI 1228 CCTCAGC CCTCAGC(-5/-2)
2296 GCTGAGG
BcgI 760 CGAGCGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1400 CGAGATGCCTGC
BlnI 21 CCTAGG C^CTAGG
164 CCTAGG
BlpI 1604 GCTGAGC GC^T[ACGT]AGC
2153 GCTAAGC
Bpu1102I 1604 GCTGAGC GC^T[ACGT]AGC
2153 GCTAAGC
BsgI 2359 CTGCAC GTGCAG(16/14)
1509 GTGCAG
BsiHKAI 573 GTGCAC G[AT]GC[AT]^C
1292 GAGCTC
BspMI 1570 ACCTGC ACCTGC(4/8)
1511 GCAGGT
BstUI 91 CGCG CG^CG
353 CGCG
CelII 1604 GCTGAGC GC^T[ACGT]AGC
2153 GCTAAGC
EaeI 746 TGGCCG [CT]^GGCC[GA]
874 TGGCCA
HgaI 89 GACGC GACGC(5/10)
1256 GACGC
MvnI 91 CGCG CG^CG
353 CGCG
PflFI 258 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2385 GACTCTGTC
PvuII 2063 CAGCTG CAG^CTG
2282 CAGCTG
RsaI 404 GTAC GT^AC
584 GTAC
SmaI 76 CCCGGG CCC^GGG
118 CCCGGG
Tth111I 258 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2385 GACTCTGTC
XmaCI 76 CCCGGG C^CCGGG
118 CCCGGG
XmaI 76 CCCGGG C^CCGGG
118 CCCGGG
XmnI 668 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1577 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcyI AflIII AluI AlwI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NarI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I