##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I ApaLI AscI
Asp718I AspEI AspI AviII BbeI
BbrPI BcgI BfrI BglII BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspEI BsrGI
BstBI BstEII BstPI CelII CpoI
DraIII EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MfeI MroI MunI
NarI NheI NotI NruI NspV
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI PvuII
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspBI StuI SwaI
Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 2061 GTATAC GT^[AC][GT]AC
AcyI 2928 GACGCC G[GA]^CG[CT]C
Aor51HI 874 AGCGCT AGC^GCT
ApaI 49 GGGCCC GGGCC^C
Asp700I 1026 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 921 GGATCC G^GATCC
BanI 2466 GGCACC G^G[CT][GA]CC
BciVI 2015 GGATAC GTATCC(6/5)
BglI 1846 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 2928 GACGCC G[GA]^CG[CT]C
BsaI 278 GAGACC GGTCTC(1/5)
BsgI 3018 CTGCAC GTGCAG(16/14)
BsiEI 1114 CGACCG CG[GA][CT]^CG
BsmI 2029 GCATTC GAATGC(1/-1)
BspDI 540 ATCGAT AT^CGAT
BsrBI 1021 GAGCGG CCGCTC(-3/-3)
BsrFI 750 GCCGGC [GA]^CCGG[CT]
Bst1107I 2061 GTATAC GTA^TAC
BstZ17I 2061 GTATAC GTA^TAC
Cfr10I 750 GCCGGC [GA]^CCGG[CT]
ClaI 540 ATCGAT AT^CGAT
DrdI 2323 GACCATTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 874 AGCGCT AGC^GCT
EcoNI 139 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 981 GAATTC G^AATTC
EcoT22I 2027 ATGCAT ATGCA^T
HaeII 874 AGCGCT [GA]GCGC^[CT]
Hin1I 2928 GACGCC G[GA]^CG[CT]C
MluI 2609 ACGCGT A^CGCGT
MspA1I 1369 CAGCGG C[AC]G^C[GT]G
NaeI 750 GCCGGC GCC^GGC
NcoI 647 CCATGG C^CATGG
NgoMIV 750 GCCGGC G^CCGGC
NsiI 2027 ATGCAT ATGCA^T
SapI 582 GAAGAGC GCTCTTC(1/4)
SbfI 1251 CCTGCAGG CCTGCA^GG
SmaI 2657 CCCGGG CCC^GGG
Sse8387I 1251 CCTGCAGG CCTGCA^GG
SspI 422 AATATT AAT^ATT
XbaI 2304 TCTAGA T^CTAGA
XmaCI 2657 CCCGGG C^CCGGG
XmaI 2657 CCCGGG C^CCGGG
XmnI 1026 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 2529 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2673 CAGTGCCTG
AocI 1015 CCTCAGG CC^T[ACGT]AGG
2545 CCTTAGG
AspHI 796 GAGCTC G[AT]GC[AT]^C
2494 GAGCTC
AvrII 352 CCTAGG C^CTAGG
2735 CCTAGG
BalI 618 TGGCCA TGG^CCA
1799 TGGCCA
BbsI 639 GAAGAC GAAGAC(2/6)
1490 GAAGAC
BclI 1569 TGATCA T^GATCA
3007 TGATCA
BlnI 352 CCTAGG C^CTAGG
2735 CCTAGG
BpuAI 639 GAAGAC GAAGAC(2/6)
1490 GAAGAC
BsiHKAI 796 GAGCTC G[AT]GC[AT]^C
2494 GAGCTC
BsmFI 1868 GTCCC GGGAC(10/14)
2655 GTCCC
BspLU11I 219 ACATGT A^CATGT
2616 ACATGT
BspMI 2486 GCAGGT ACCTGC(4/8)
2528 GCAGGT
BssHII 42 GCGCGC G^CGCGC
66 GCGCGC
BssSI 1 CTCGTG CACGAG(-5/-1)
728 CTCGTG
BstAPI 2363 GCACATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2987 GCAGAGGTTGC
Bsu36I 1015 CCTCAGG CC^T[ACGT]AGG
2545 CCTTAGG
DraI 2100 TTTAAA TTT^AAA
2399 TTTAAA
DsaI 647 CCATGG C^C[GA][CT]GG
1847 CCGTGG
Eco81I 1015 CCTCAGG CC^T[ACGT]AGG
2545 CCTTAGG
FbaI 1569 TGATCA T^GATCA
3007 TGATCA
MluNI 618 TGGCCA TGG^CCA
1799 TGGCCA
MscI 618 TGGCCA TGG^CCA
1799 TGGCCA
NdeI 1070 CATATG CA^TATG
1337 CATATG
PflMI 266 CCACAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1911 CCAGGAGGTGG
PpuMI 159 AGGACCC [GA]G^G[AT]CC[CT]
2653 GGGTCCC
PstI 1252 CTGCAG CTGCA^G
2768 CTGCAG
SacI 796 GAGCTC GAGCT^C
2494 GAGCTC
Van91I 266 CCACAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1911 CCAGGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AvaI AvaII BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsoBI
Bsp1286I BspHI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PacI PleI PshBI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII