##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AcyI AflIII
AgeI AhdI AocI AscI AseI
AsnI Asp718I AspEI AviII BbeI
BbrPI BciVI BclI BglII BlpI
Bpu1102I BsaBI BsaHI BsiEI BsiWI
BsmI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
CpoI DraIII EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoT22I FbaI
FseI FspI Hin1I HindIII KasI
KpnI MamI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
PvuI RcaI RsrII SacI SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SpeI SphI Sse8387I StuI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccIII 1301 TCCGGA T^CCGGA
AclI 1966 AACGTT AA^CGTT
AflII 2576 CTTAAG C^TTAAG
Alw44I 1893 GTGCAC G^TGCAC
AlwNI 825 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 337 GGGCCC GGGCC^C
ApaLI 1893 GTGCAC G^TGCAC
Asp700I 2638 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 385 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2294 CCTAGG C^CTAGG
BbvCI 2114 GCTGAGG CCTCAGC(-5/-2)
BcgI 61 CGATAAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2576 CTTAAG C^TTAAG
BlnI 2294 CCTAGG C^CTAGG
BsaAI 2060 TACGTA [CT]AC^GT[GA]
BseAI 1301 TCCGGA T^CCGGA
BsgI 948 CTGCAC GTGCAG(16/14)
BsmBI 1827 CGTCTC CGTCTC(1/5)
Bsp1407I 1921 TGTACA T^GTACA
BspDI 2027 ATCGAT AT^CGAT
BspEI 1301 TCCGGA T^CCGGA
BspMI 635 ACCTGC ACCTGC(4/8)
BsrFI 1369 GCCGGC [GA]^CCGG[CT]
BsrGI 1921 TGTACA T^GTACA
Cfr10I 1369 GCCGGC [GA]^CCGG[CT]
ClaI 2027 ATCGAT AT^CGAT
DraI 2182 TTTAAA TTT^AAA
DrdI 1443 GACCATTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1010 CCTTGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1378 GAATTC G^AATTC
EcoRV 400 GATATC GAT^ATC
HpaI 2552 GTTAAC GTT^AAC
HphI 1182 GGTGA GGTGA(8/7)
KspI 2 CCGCGG CCGC^GG
MroI 1301 TCCGGA T^CCGGA
NaeI 1369 GCCGGC GCC^GGC
NcoI 332 CCATGG C^CATGG
NgoMIV 1369 GCCGGC G^CCGGC
PflFI 385 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1536 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1966 AACGTT AA^CGTT
PstI 996 CTGCAG CTGCA^G
PvuII 900 CAGCTG CAG^CTG
SacII 2 CCGCGG CCGC^GG
SapI 1146 GCTCTTC GCTCTTC(1/4)
SmaI 131 CCCGGG CCC^GGG
SnaBI 2060 TACGTA TAC^GTA
SspBI 1921 TGTACA T^GTACA
SspI 2688 AATATT AAT^ATT
Tth111I 385 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1536 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 344 CCAGCGTCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 131 CCCGGG C^CCGGG
XmaI 131 CCCGGG C^CCGGG
XmnI 2638 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 3 CGCG CG^CG
447 CGCG
Aor51HI 755 AGCGCT AGC^GCT
1508 AGCGCT
AvaI 131 CCCGGG C^[CT]CG[GA]G
1326 CCCGAG
AvaII 50 GGACC G^G[AT]CC
1696 GGTCC
BalI 18 TGGCCA TGG^CCA
1528 TGGCCA
BamHI 2141 GGATCC G^GATCC
2281 GGATCC
BanII 134 GGGCTC G[GA]GC[CT]^C
337 GGGCCC
BglI 298 GCCACCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1132 GCCTGCTGGGC
BsaI 1441 GAGACC GGTCTC(1/5)
378 GGTCTC
BsaWI 1301 TCCGGA [AT]^CCGG[AT]
1502 ACCGGA
BsoBI 131 CCCGGG C^[CT]CG[GA]G
1326 CCCGAG
BstUI 3 CGCG CG^CG
447 CGCG
EaeI 18 TGGCCA [CT]^GGCC[GA]
1528 TGGCCA
Eco47III 755 AGCGCT AGC^GCT
1508 AGCGCT
MluNI 18 TGGCCA TGG^CCA
1528 TGGCCA
MscI 18 TGGCCA TGG^CCA
1528 TGGCCA
MslI 22 CATTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1532 CACCCCAGTG
MvnI 3 CGCG CG^CG
447 CGCG
PpuMI 49 GGGACCC [GA]G^G[AT]CC[CT]
1695 AGGTCCC
PshAI 1805 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2390 GACATCTGTC
ScaI 1214 AGTACT AGT^ACT
2483 AGTACT
SfcI 996 CTGCAG C^T[GA][CT]AG
2036 CTATAG
TfiI 1001 GAATC G^A[AT]TC
2439 GATTC
Tsp45I 1193 GTGAC ^GT[GC]AC
2168 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII