##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BcgI BcnI BglI BlnI BsaBI
BsaHI BsaWI BseAI BsiWI BspEI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstZ17I Cfr10I CpoI
Eam1105I Eco47III EcoNI FseI FspI
HapII Hin1I HpaII KasI KpnI
KspI MamI MluI MluNI MroI
MscI MspI NaeI NarI NciI
NgoMIV NheI NruI NspV PacI
PflFI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I Tth111I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 7755 GTAGAC GT^[AC][GT]AC
AccII 1112 CGCG CG^CG
AvaI 4654 CTCGAG C^[CT]CG[GA]G
BbrPI 2576 CACGTG CAC^GTG
BciVI 697 GTATCC GTATCC(6/5)
BlpI 7000 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 7000 GCTAAGC GC^T[ACGT]AGC
BsiEI 55 CGGCCG CG[GA][CT]^CG
BsmBI 1108 GAGACG CGTCTC(1/5)
BsoBI 4654 CTCGAG C^[CT]CG[GA]G
BspDI 226 ATCGAT AT^CGAT
BspMI 757 GCAGGT ACCTGC(4/8)
BsrDI 1123 GCAATG GCAATG(2/0)
BstAPI 6712 GCATCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1218 GGTGACC G^GT[ACGT]ACC
BstPI 1218 GGTGACC G^GT[ACGT]ACC
BstUI 1112 CGCG CG^CG
BstXI 7702 CCAGGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 7000 GCTAAGC GC^T[ACGT]AGC
CfoI 752 GCGC GCG^C
ClaI 226 ATCGAT AT^CGAT
DraIII 1520 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 115 GACAATAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 55 CGGCCG C^GGCCG
EclXI 55 CGGCCG C^GGCCG
Eco52I 55 CGGCCG C^GGCCG
EcoO65I 1218 GGTGACC G^GT[ACGT]ACC
EcoRV 7194 GATATC GAT^ATC
HaeII 751 GGCGCT [GA]GCGC^[CT]
HgaI 1110 GACGC GACGC(5/10)
HhaI 752 GCGC GCG^C
HinP1I 752 GCGC G^CGC
MvnI 1112 CGCG CG^CG
NcoI 6140 CCATGG C^CATGG
NotI 54 GCGGCCGC GC^GGCCGC
PaeR7I 4654 CTCGAG C^TCGAG
PmaCI 2576 CACGTG CAC^GTG
PmlI 2576 CACGTG CAC^GTG
PshAI 4839 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2245 GAGCTC GAGCT^C
SapI 2242 GAAGAGC GCTCTTC(1/4)
SspI 3166 AATATT AAT^ATT
SwaI 5549 ATTTAAAT ATTT^AAAT
XhoI 4654 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflII 4831 CTTAAG C^TTAAG
5413 CTTAAG
AocI 5010 CCTTAGG CC^T[ACGT]AGG
7721 CCTCAGG
BanI 1283 GGTGCC G^G[CT][GA]CC
6718 GGTGCC
BanII 1115 GGGCTC G[GA]GC[CT]^C
2245 GAGCTC
BbvCI 7213 CCTCAGC CCTCAGC(-5/-2)
2214 GCTGAGG
BfrI 4831 CTTAAG C^TTAAG
5413 CTTAAG
BsgI 930 CTGCAC GTGCAG(16/14)
7847 GTGCAG
BsmFI 810 GGGAC GGGAC(10/14)
2774 GGGAC
BsmI 7913 GAATGC GAATGC(1/-1)
7789 GCATTC
Bsp1407I 3375 TGTACA T^GTACA
7260 TGTACA
BsrGI 3375 TGTACA T^GTACA
7260 TGTACA
Bsu36I 5010 CCTTAGG CC^T[ACGT]AGG
7721 CCTCAGG
DsaI 32 CCGTGG C^C[GA][CT]GG
6140 CCATGG
EaeI 35 TGGCCG [CT]^GGCC[GA]
55 CGGCCG
Eco81I 5010 CCTTAGG CC^T[ACGT]AGG
7721 CCTCAGG
FauI 1113 GCGGG CCCGC(4/6)
2157 GCGGG
MfeI 1138 CAATTG C^AATTG
3142 CAATTG
MslI 175 CATGGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
5218 CACTCAAATG
MunI 1138 CAATTG C^AATTG
3142 CAATTG
NspI 5120 ACATGC [GA]CATG^[CT]
5279 ACATGC
SspBI 3375 TGTACA T^GTACA
7260 TGTACA
StuI 3627 AGGCCT AGG^CCT
7650 AGGCCT
XbaI 1632 TCTAGA T^CTAGA
5944 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BbsI BbvI
BclI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FbaI Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HpaI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeI NdeII NlaIII NlaIV
NsiI PflMI PleI PpuMI PshBI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI